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Esther Chua | Bench E | 31 Aug 2016

Classification

Higher order taxa

Bacteria - Proteobacteria - Betaproteobacteria - Neisseriales - Neisseriaceae - Kingella - Kingella oralis

Species

Kingella oralis

Type strain: strain UB-38 = ATCC 51147= CCUG 30450 = CIP 103803

Description and significance

Kingella oralis was first obtained from human periodontal isolates recovered from a purported Eikenlla corrodens-selective medium¹, and was originally described by Chen et al.(1990) as Eikenella corrodens-like isolates. Using 16sRNA sequencing, this species was identified as K. orale in 1993¹, but the spelling was changed to "K. oralis" in 1994². K. oralis is a gram-negative facultative (aerobic and anaerobic growth) organism thats grows in mesophillic environments. K. oralis is normally found in small numbers in the oral cavity in dental plaque, however periodontitis and gingivitis may correlate with increases in numbers of this species in the gingiva. They also produce corroding colonies. K. oralis is rather prevalent in dental plaque and has shown to constitute more than 5% of the total microbiota in peridontitis sites³.

Genome structure

Kingella Oralis strain ATCC 51147 does not encode for chromosomes and plasmids. It has a total length of about 2.41mb, encodes for 2,315 proteins and has a GC content of 54.3%. Sequencing results show that ATCC51147 consists of 5 scaffolds (Scfld 0-4) with no gaps between, an assembly gap length of 700, and 12 contigs. It has 3,165 coding genes and 52 non-coding genes.

Cell structure and metabolism

Kingella oralis are gram negative rods or cocobacilli that can form pairs or chains. It has a cell wall that consists of an outer membrane containing lipopolysaccharides, a periplasmic space with a peptidoglycan layer, and an inner, cytoplasmic membrane. Cells are nonmotile by flagella, but have monopolar fimbriae and form spreading colonies which suggests twitching motility¹. K. oralis were not known to coaggregate, however recent studies have shown that they are able to coadhere with Streptococcus oralis during the formation of complex biofilms⁷. Cells can be aerobic or facultatively anaerobic. They are oxidase positive, catalase negative and are able to ferment glucose.

Ecology

K. oralis is an aerobic or facultatively anaerobic organism that is often cultured for on blood agar. The main habitat of K. oralis is in human dental plaque, however, it can also be found in smaller quantities in salival and mucosal sites. In periodontally healthy patients, K. Oralis is relatively evenly distributed in subgingival and supragingival plaque, but it has a higher distribution in the supragingival plaque of those with periodontitis ⁹.

The Kingella species are known to exchange antibiotic resistance plasmids with Neisseria gononhoeae and Neisseria meningitidis, resulting in a possibility of K. oralis as a plasmid reservoir¹⁰.

Pathology

K. oralis is an organism that is capable of locally invading periodontal tissues and inducing host defense. This contributes to gum diseases such as gingivitis and periodontitis that affects both adults and juveniles⁹.

Gingivitis is usually the precursor stage of gum disease and is caused by gum inflammation due to K. oralis and other bacteria accumulating as plaque or tartar on teeth. In gingivitis, the gums become red, swollen, and bleeds easily. However, with regular cleaning of teeth, gingivitis can usually be reversed.

When gingivitis is left untreated, there is a possibility of periodontitis (inflammation around the tooth) occurring. This causes gums to pull away from the teeth to form pockets that become infected. Bacterial toxins produced combined with the body's immune response to the infection causes the breakdown of bone and connective tissue that hold the teeth in place. When left untreated, the teeth may eventually become loose and would have to be removed. However, by maintaining good oral hygiene and practices, gum diseases such as these can be controlled.⁸

Application to biotechnology

K. oralis has been used in the validation of a new assay that can be used in the screening for coadhesion partners among the multitude of species present in oral biofilms⁷.

There has been no other evidence that shows that this species has been used in biotechnology.

Current research

K. oralis was not known to coaggregate with other species during biofilm formation, however recent research investigating the interbacterial adhesion in dental plaque development has identified K. oralis to be able to coaggregate with Streptococcus oralis during the formation of complex biofilms⁷.

Another recent research on DNA Uptake Sequence (DUS) has discovered that K. oralis replication is DUS-dependent(kingDUS) and this affects transformation by limiting DNA uptake and recombination in favour of homologous DNA. Approximately 2.5% of the K. oralis genome is occupied by the kingDUS, and this is very high compared to the approximate 1% DUS occupancy in N. meningitidis and N. gonorrhoeae genomes⁶.

References

1. Dewhirst F, Chen C, Paster B, Zambon J. 1993. Phylogeny of Species in the Family Neisseriaceae Isolated from Human Dental Plaque and Description of Kingella orale sp. nov. International Journal of Systematic Bacteriology 43:490-499

2. Bergey D, Boone D, Brenner D, Castenholz R, De Vos P, Garrity G, Krieg N, Staley J. 2001. Bergey's Manual of systematic bacteriology. Springer, New York, p.839

3. Chen C. 1996. Distribution of a newly described species, Kingella oralis, in the human oral cavity. Oral Microbiology and Immunology 11:425-427

4. NCBI-Genome

5. Yagupsky, P. Kingella species. Antimicrobe

6. Frye S, Nilsen M, Tønjum T, Ambur O. 2013. Dialects of the DNA Uptake Sequence in Neisseriaceae. PLoS Genetics 9:e1003458

7. Ruhl S, Eidt A, Melzl H, Reischl U, Cisar J. 2014. Probing of Microbial Biofilm Communities for Coadhesion Partners. Applied and Environmental Microbiology 80:6583-6590

8. 2014. Periodontal (Gum) Disease: Causes, Symptoms, and Treatments. National Institute of Dental and Craniofacial Research

9. Chen C. 1996. Distribution of a newly described species, Kingella oralis, in the human oral cavity. Oral Microbiology and Immunology 11:425-42

10. Knapp, J. S., S. R Johnson, J. M. Zenilman, M. C. Roberts, and S. A. Morse. 1988. High-level tetracycline resistance resulting from TetM in strains of Neisseria spp., Kingella denitrificans, and Eikenella corrodens. Antimicrob. Agents Chemother. 32:765-767

This page is written by <Esther Chua> for the MICR3004 course, Semester 2, 2016