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Aimee Davidson Bench E 23 Sept 2016

Classification

Higher order taxa

Bacteria - Firmicutes - Negativicutes - Selenomonadales - Veillonellaceae - Veillonella^[3]

Species

Species name: Veillonella parvula (subspecies parvula) ^[3]

Type strain: ATCC 10790 = CCUG 5123 = DSM 2008 = JCM 12972 = NCTC 11810 ^[3]

Description and significance

Veillonella parvula is one of thirteen species of the Veillonella genus and one of only six of these species to be found within the human oral cavity ^[4]. V.parvula was originally dubbed Staphylococcus parvulus, by Veillon and Zuber in 1898, who first isolated and cultured the bacteria from an infected human appendix ^[5]. It was subsequently further described and renamed Veillonella parvula in 1933 by Prevot [11]. V. parvula is a small gram-negative cocci, typically between 0.3-0.5 microns in diameter -which can be identitified by its characteristic pair grouping and distinctive red fluorescence under UV light and certain growth conditions ^[6]. As mentioned previously it is commonly found within the human oral cavity, specifically on the tongue, buccal mucosa and in the saliva, however it can also be found within the human gastrointestinal, genitourinary and respiratory tracks^[7], ^[8]. Initially, V. parvula was thought to be non-pathogenic accounting for up to 98 % of cultivable Veillonella species in healthy human oral cavities ^[7]. However, it is now known that while it is typically not directly pathogenic it regularly coaggregates with other pathogenic species, such as streptococcus and is a key early colonizer for the formation of oral biofilms ^[4].

Scanning electron micrograph image of V. parvula ^[5]

Genome structure

V. parvula exhibits a circular 2.13 Mb (2,132,142 bp) genome, of which 88.46 % is DNA encoding, and was the first member of the Veillonella genus to have its complete genome sequenced. Its 1920 genes include: 1859 protein-encoding genes, 61 RNA specific genes and 15 pseudogenes, with an overall GC content of 38.63%. The majority of these genes are predicted to have either an unknown (385 genes) or general function (177 genes). Those genes with a predicted function are predominately involved with: translation, ribosomal structure, cell wall or membrane biogenesis, energy production and conversion, amino acid, coenzyme and inorganic transport and metabolism^[7].

Genome structure of V. parvula ^[7].

Cell structure and metabolism

Verkley et al established that 98 % of the phosphoglycerides that comprise V. parvula's cell membrane are either serine or ethanolamine based. These phosphoglycerides typically exist in single bonded carbon chains consisting of between 11 to 19 carbons, although chains containing 15,17,18 or 19 carbons atoms may also contain one double carbon bond [12]. Additionally, many of these phosphoglycerides exist in a plasmogen form which is thought to have an important role in the regulation of the cell's membrane fluidity^[6],^[9]. The peptidoglycan cell wall of V. parvula consists of: glutamic acid in the D configuration; diaminopimelic acid in the meso configuration; and alpha-linked cadaverine and putrescine to glutamic acid^[7]. V. parvula lack flagella, spores and capsules and the ability to adhere to surfaces [6, 5]. It overcomes this lack of surface adhesion by adhering to specific surface structures on other bacterial species through lectin-carbohydrate mediated interactions^[7]. V. parvula is unable to utilise carbohydrates, such as glucose, or amino acids in its energy production but rather utilises the metabolic end products of closely associated bacteria within the environment, particularly lactic acid producing species^[7],^[5]. That is,it is able to metabolise lactate, pyruvate, malate and fumarate to produce propionate, acetic acid, carbon dioxide (CO2) and hydrogen^[7]. In addition, Ng and Hamilton established that V. parvula produces greater quantities of CO2 and propionate, which is explicitly shown in the following general equation of lactate metabolism: 8 lactate metabolises to 5 propionate + 3 acetate + 3 CO2 + H2

Ecology

V. parvula is a strict anaerobe that ideally grows at 37C and a pH range of 6.5 to 8.0, as such humans present as the ideal host. It is most commonly found within the human oral cavity and gastrointestinal tract, although it may also be found within the genitourinary and respiratory tracks^[7],^[8]. Owing to V. parvula’s inability to directly adhere to host surfaces it has no direct microbe to host interactions, rather it relies upon interactions with other microbes within the same biological niche^[5]. Specifically it is commonly a key early colonizer in early oral biofilm formation and facilitates the colonizing of middle and late phase pathogenic colonizers responsible for direct host/microbe interactions^[4].

Pathology

V. parvula has a well-established association with human oral diseases, in particular demonstrating a strong association with severe early childhood carries and intraradicular infections^[4],^[7]. Recent research has also revealed that V. parvula is the predominant Veillonella species within dentine biofilms^[11]. Although V. parvula generally presents as a commensal or non-pathogenic resident in the bacterial communities of the oropharynx -, the gastrointestinal, genitourinary and respiratory tracks it has rarely been associated with a number of serious and life-threatening infections. These infections include: osteomyeltitis, spondylodiscitis, discitis, meningitis and sinus infections^[6],^[8]. It has demonstrated resistance against a wide range of antibiotics including: tetracycline, erythromycin, gentamicin, karamycin, chloramphenicol and lincomycin. Although importantly at the time of its genome sequencing in 2010 it was still susceptible to penicillin, cephalotin and clindamycin^[7].

Application to biotechnology

Perhaps largely owing to V. parvula’s largely commensal nature, research thus far has not focused upon exploring its use in the field of bioengineering. Although a recent study by Periasamy and Kolenbrander has highlighted its potential as therapeutic target particularly with regards to dental plaque formation. They revealed that V. parvula was especially required for the development of three species biofilms. Thereby suggesting that prevention of V. parvula colonisation may hinder the development of these biofilms that contribute to human oral disease^[12].

Current research

A key advancement towards the study of V. parvula was made recently in June 2016. Historically species specific identification of members of Veillonella genus has been difficult owing to a lack of sufficiently reliable phenotypic and biochemical tests. Particularly the well-established identification method using 16S rDNA technology has proved unreliable. Mashima et al was able to overcome this issue by developing a novel one-step multiplex PCR method utilising purpose designed primer sets that target regions within the rpoB gene of individual Veillonella species^[4].

References

References examples

1. Sahm, K., MacGregor, B.J., Jørgensen, B.B., and Stahl, D.A. (1999) Sulphate reduction and vertical distribution of sulphate-reducing bacteria quantified by rRNA slotblot hybridization in a coastal marine sediment. Environ Microbiol 1: 65-74.

2. Human Oral Microbiome

3. List of prokaryotic names with standing in nomenclature

4. Izumi Mashima,1,2 Citra Fragrantia Theodorea,2,3 Boonyanit Thaweboon,4 Sroisiri Thaweboon,4 and Futoshi Nakazawa2 (2016) Identification of Veillonella Species in the Tongue Biofilm by Using a Novel One-Step Polymerase Chain Reaction Method. PLoS One. 11(6):e0157516

5. Delwiche EA, Pestka JJ, Tortorello ML (1985) The veillonellae: gram-negative cocci with a unique physiology. Ann. Rev. Microbiol 39:175-93

6. Verkley AJ, Ververgaert PH, Prins RA, van Golde LM (1975) Lipid-phase transitions of the strictly anaerobic bacteria Veillonella parvula and Anaerovibrio lipolytica. J Bacteriol 124(3):1522-8

7. Sabine Gronow, Sabine Welnitz, Alla Lapidus,2 Matt Nolan,2 Natalia Ivanova,2 Tijana Glavina Del Rio,2 Alex Copeland,2 Feng Chen,2 Hope Tice,2 Sam Pitluck,2 Jan-Fang Cheng,2 Elizabeth Saunders,2,3 Thomas Brettin,2,3 Cliff Han,2,3 John C. Detter,2,3 David Bruce,2,3 Lynne Goodwin,2,3 Miriam Land,2,4 Loren Hauser,2,4 Yun-Juan Chang,2,4 Cynthia D. Jeffries,2,4 Amrita Pati,2 Konstantinos Mavromatis,2 Natalia Mikhailova,2 Amy Chen,5 Krishna Palaniappan,5 Patrick Chain,2,3 Manfred Rohde,6 Markus Göker,1 Jim Bristow,2 Jonathan A. Eisen,2,7 Victor Markowitz,5 Philip Hugenholtz,2 Nikos C. Kyrpides,2, Hans-Peter Klenk,1 and Susan Lucas2 (2010) Complete genome sequence of Veillonella parvula type strain (Te3). Stand Genomic Sci 2(1): 65-74.

8. Chen YC, Ko PH, Yang CJ, Chen YC, Tsai CC, Hsieh MH (2014) Epidural abscess caused by Veillonella parvula: Case report and review of the literature. J Microbiol Immunol Infect. pii:S1684-1182(14)00098-X

9. Bhatti MA, Frank MO (2000) Veillonella parvula meningitis: case report and review of Veillonella infections. Clin Infect Dis. 31(3):839-40.

10. Ng SK, Hamilton IR. (1971) Lactate metabolism by Veillonella parvula. J Bacteriol. 105(3):999-1005

11. Thuy Do, Sheehy EC, Tonnie M, Hughes F, Beighton D (2015) Transcriptomic analysis of three Veillonella spp. present in carious dentine and in the saliva of caries-free individuals. Front Cell Infect Microbiol. 5</b:25.

12. Periasamy S, Kolenbrander PE (2010) Central role of the early colonizer Veillonella sp. in establishing multispecies biofilm communities with initial, middle, and late colonizers of enamel. J Bacteriol 192(12):2965-2972

This page is written by Aimee Davidson for the MICR3004 course, Semester 2, 2016