Bacillus Subtilis Soil Project: Difference between revisions
| Line 23: | Line 23: | ||
Date: 1.25.2018 | Date: 1.25.2018 | ||
Temperature: 58° F | Temperature: 58° F | ||
Recent rainfall: 0 inches | Recent rainfall: 0 inches | ||
Depth: Surface to 2” | Depth: Surface to 2” | ||
Grid coordinates: 30.20144°, -97.88822° | Grid coordinates: 30.20144°, -97.88822° | ||
Soil type number and name from NRCS soil map: | Soil type number and name from NRCS soil map: | ||
Name: Volente silty clay loom, 1 to 8 percent slopes | Name: Volente silty clay loom, 1 to 8 percent slopes | ||
Unit key and symbol: 39325833, f66r | Unit key and symbol: 39325833, f66r | ||
Description: The location the organism was isolated was a grassy field between a soccer field, parking lot, and children’s playground. There was full sun, little traffic near the area, and used often by local residents from the suburban area. | Description: The location the organism was isolated was a grassy field between a soccer field, parking lot, and children’s playground. There was full sun, little traffic near the area, and used often by local residents from the suburban area. | ||
Revision as of 03:08, 1 May 2018
Classification
Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]
Domain: Bacteria Phylum: Firmicutes Class: Bacilli Order: Bacillales Family: Bacillaceae
Species
|
NCBI: Taxonomy |
Bacillus subtilis
Habitat Information
Description of location and conditions under which the organism was isolated:
Date: 1.25.2018
Temperature: 58° F
Recent rainfall: 0 inches
Depth: Surface to 2”
Grid coordinates: 30.20144°, -97.88822°
Soil type number and name from NRCS soil map:
Name: Volente silty clay loom, 1 to 8 percent slopes
Unit key and symbol: 39325833, f66r
Description: The location the organism was isolated was a grassy field between a soccer field, parking lot, and children’s playground. There was full sun, little traffic near the area, and used often by local residents from the suburban area.
Description and Significance
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.
Genome Structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.
The organism has 4215606 base pairs. Its chromosome is circular.
HMN1-Forward_A06.ab1 937 letters, trimmed about 40 b/p
TTGNNGCGTANGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAAT TCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAG ATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGAC TGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATA GGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGC ATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGA ACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAG CATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTAGGAGCCAGCCGCCGAANGTGG GACAGATGATTGGGGTGAANTCGTA
HMN2-Reverse_B06.ab1 936 letters, trimmed about 20 b/p TCGGNGGNTGGCTCCTAAAAGGTTACCTCACCGACTTCGGGTGTTACAAACTCTCGTGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATC CGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTC CATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTGAATGCTGGC AACTAAGATCAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCACTCTGCCCCCGAAGGGGACGTCCTATCTC TAGGATTGTCAGAGGATGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAGTCTTG CGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTAAGGGGCGGAAACCCCCTAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAAT
Cell Structure, Metabolism and Life Cycle
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Physiology and Pathogenesis
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
References
Author
Page authored by _____, student of Prof. Kristine Hollingsworth at Austin Community College.
