Equine Development of Gut Microbiota

A young horse, called a foal. Photo credit: [1]

By Mira Allen

Topic: development of and mature gut microbiota in equine

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Introduction and Importance

Much like in humans, the equine gut microbiome is considered by some to be akin to an organ system due to the role it plays in digestion [10]. An intact microbiota ensures mucosal immunity, produce short-chain fatty acids, maintains antigen tolerance, prevents colonization of harmful organisms such as infectious agents, stimulates mucus production, and improves dendrite function[24].

In utero, horses' intestinal tracts are close to sterile. Soon after birth, however, microbial colonization skyrockets. Colonization of the gut with bacteria that can digest an adult diet, including roughage, lasts about 60 days [1,10]. Proper colonization is incredibly important as improper microbial gut content can result in dysbiosis, causing inflammation and metabolic disease [1,2]. Equine hind-gut microbiota enable nutritional optimiztion from an otherwise nutrient-poor foraging diet via plant material fermentation [2]. The initial colonization, stabilization, and then weaning period (4-6 months old) as a foal transfers to solid food are important periods in establishing the microbial composition of the colon [1].

Most equine gut bacteria live in the colon, specifically in the caecum [2]. These bacteria degrade otherwise indigestible forage [2]. The equine gut is composed of two main sections:

Upper/fore gut
This area is made up of the stomach, jejunum, and ileum [2]. It is associated with increased microbiota variation and turnover as food is processed here first [2].

Lower/hind gut
This area is made up of the anaerobic caecum and colon [2,24]. The microbiota here is more stable, with most residing in the colon [2]. Here, bacteria ferment structural carbohydrates, allowing energy to be extracted from forage [24].

Gut Colonization: Birth to Maturity

The stages of microbiome colonization in mammals are as follows:
- Initial colonization [1]
- Microbiota stabilization [1]
- Weaning (in foals: between 4 and 10 months) [1,24]
- Transfer to solid foods (occurs gradually) [1]

While these are generally applicable in mammals, it has been demonstrated that the foal gut microbiome closely resembles their mother's around 60 days of age [1], although weaning has been shown in some cases to have an impact (though this may be due to stress)[24]. This means that their microbiome development occurs more rapidly than most other mammals. A foal’s mother begins to influence its microbiome beginning in utero by circulating microbial metabolites, but the birthing process has a larger impact on colonization [1,3]. Foals have bacteria present in their system immediately after birth. Their microbiome is then diversified and refined via the consumption of mare's milk and manure [1]. The first week of life is a critical time as they are susceptible to infection and disease [3]. Diarrhea is common in this period and is possibly associated with a microbial imbalance [3].

Due to the low protein permeability of the placenta, foals are born without circulating immunoglobulins [8]. Thus, their immune system is incredibly weak until passive immunity is transferred via immunoglobulins in the colostrum [8]. Colostrum is the first milk produced following the birth of the foal. Its consumption is incredibly important in the initial colonization stage. The transition from colostrum to mature milk production begins around 2 days post-parturition and lasts several weeks [8]. It is nutrient rich and provides the foal with amino acids, proteins, immunological factors, and antioxidants [8]. This is vital to the foal’s growth, development, and immunocompetence and immune development [8].

Immunoglobulins gained from colostrum protect the foal from environmental pathogens and make up nearly 60% of this milk's protein content [8]. They are present in colostrum at much higher concentrations than mature milk and therefore immunity is transferred to the foal only in the first twelve hours of life [8]. Immunoglobulin G occurs at high concentrations in colostrum, and immunoglobulin A at lower ones. Mature mare’s milk contains mostly Immunoglobulin A [8]. Also present in equine milk are proteins lysozyme and lactoferrin [8,10]. These possess antimicrobial properties and to protect foals from bacterial infection, and furthermore may aid in proper gut microbiome establishment, including lactobacillus and bifidobacterium [8]. Bacteria from Deltaproteobacteria, Desulfovibrionaceae, and Erysipelotrichaceae are more commonly seen in diets high in fat[12,13], as one would see in foals drinking exclusively milk [10].

Before 60 days old, gut microbiota composition is transient and dynamic. Research [1] has shown that change in similarity between individual foals and foals and adults occurs in these stages:
Day 0: relatively diverse bacterial colonization likely inherited from the foal's dam (mother) and the environment [3]
Day 1: somewhat decreased microbiota diversity [3]
Day 7: large variation between the microbiota of individual foals
Day 20: more consistent between foals, but different from adults
Day 60: very similar to adult composition

A decrease in microbiome diversity has been demonstrated as horses age, which have been speculatively attributed to changes in diet and/or deteriorating dental quality, digestion efficiency, and energy requirements[24].

Species Composition

Over 1,000 species of bacteria have been identified thus far in the equine GI tract [24]. The core mature equine microbiome (60+ days) that has so far been documented to include:

- Bacteroidetes (one of the most abundant phyla) [2] including:
- Bacteroides uniformis [1]
- Bacteroides fragilis [1]
- Parabacteroides [1]
- Butyricimonas [1]

- Firmicutes (largest and one of the most abundant phyla of intestinal bacteria, including clostridia and bacilli) [2]
- Clostridiales (part of the intestinal core microbiome in all mammals) [2]
- Produce butyrate (protective of colonocytes) [2]
- Lactobacillus mucosae [1]
- Blautia producta [1]
- Streptococcus [1]
- Ruminococcaceae (small percentage, hindgut) [2]
- Fibrobacteraceae (small percentage, hindgut) [2]

Ruminococcaceae and Fibrobacteraceae are involved in fibrous plant wall degradation and are an integral species in gut health [2,10]. They also produce anti-inflammatory and immune-modulating short chain fatty acids [24]. Some gut Clostridia are necessary for regular processes including physiology, metabolism, and immunity [16], but high levels may cause inflammation [17].

- Proteobacteria (second largest phylum, driven by environment uptake)(common in upper GIT, most in ileum) may play a role in nitrogen fixation, but overabundance can cause inflammation, sometimes colic. [2]
- Enterobacteriales [1][2]
- Pseudomonadales [2]

- Verrucomicrobia (third largest phylum, present in the caecum, small colon, and rectum)[2](assist in hindgut fermentation [10])
- Akkermansia (mucin degrading, maintains mucin layer integrity, decreases bowel inflammation) [2]

- Spirochaetes
- Spirochaetaceae [18]
- Lachnospiraceae [24]

Also present in the hindgut [2]:
- Protozoa [2]
- Fungi [2]
- Yeast [2]
- Archaea [2]
- Methanogenic archaea (metabolize H2 and CO2 to methane; support degradation of cellulolytic bacteria) [2]

The foal gut microbiome has been recorded to contain mostly Firmicutes [2]. Between 2 and 30 days, Verrucomicrobia are the main species [2].

Just after birth (0hrs), the foal microbiome contains mostly Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes groups [3]. Species present include Staphylococcus, Lactobacillus, Bacillus, Streptococcus, Corynebacterium, Sphingomonas, Pseudomonas, and Acinetobacter [3,10]. This is a relatively high diversity of species that decreases after the first day [3]. A newborn foal's gut is relatively uncolonized, and this allows bacteria to take hold and rapidly reproduce [10]. Pseudomonas takes hold especially quickly due to its generalist strategy and rapid reproduction [10].

After 24 hours, the gut contains mostly Escherichia/Shigella such as E. coli, Bacteroides, and Firmicutes, with species including Clostridium, streptococcus, enterococcus, and klebsiella [3]. Some beneficialE. coli release bacteriocins which prevent the growth of other species [24]. This is beneficial as it prevents pathogenic E. coli growth [24].

By day 7, the most abundant group is firmicutues, with bacteroides and fusobacteria having increased in abundance decreased proteobacteria [3]. The increase in Bacteroides specifically has been associated with the introduction of solid food[11] as they play a part in complex protein and sugar digestion [15], which at this age foals have likely been increasingly exposed to [10]. Bacteroides were most abundant genus, present in all foals along with Fusobacterium, Tyzzerella, Streptococcus and Lactobacillus [3] Enterobacteriaceae, Erysipelotrichaceae, and Peptostreptococcaceae have also been documented at high concentrations in day 7 samples [10]. The increase in Enterobacteriaceae at this age is likely due to repeated exposure to fecal matter [10]. Both of the families Lachnospiraceae and Ruminococcaceae were present from day 7 until weaning, and have been hypothesized to aid in the breakdown of complex carbohydrates [10].

These findings may differ between individuals depending on their housing (ie. stall or turnout), access to adult foods from birth (ie. grain, grass), and geographic location. Domestication and housing type interferes with the sharing of microbiomes between horses. Domesticated horses have lower clostridia phascolarctobacterium, which produces short chain fatty acid propionate [2]. Furthermore, non-domestic horses have higher levels of methanocorpusculum archaea, which are methane producers and may increase the carbohydrate degrading activity of cellulolytic bacteria [2].

Similarly, horses fed mainly grain as opposed to forage were found to have lower levels of Fibrobacter and Ruminococcaceae and higher levels of Lachnospiraceae, Bacteroidetes, and members of the Bacillus-Lactobacillus-Streptococcus group [24]. It has even been suggested that the "gut-brain" relationship discussed so often in humans may also exist in horses. High-fiber diets in horses have been associated with calmer, more investigative behavior compared to increased anxiety seen in horses consuming high-starch diets [24].

An Unhappy Microbiome: Effects of Change and Dysbiosis

Harmful changes to the equine gut microbiome may occur as a result of:
- Stress [24]
- Transport [2]
- Fasting [2]
- Abrupt dietary change [24]
- Antibiotic administration [24]

An imbalance resulting in a deficiency of one or multiple species can result in the overgrowth of another. It can take up to 25 days to bring the biome back to baseline, but effects can last longer [2].

Transporting horses has shown to be associated with decreased levels several bacteria including Clostridia, Bacteroidetes, and Streptococci, but this response can also be partially attributed to stress [24]. General stress has been associated with a decrease in Bacteroidaceae [19] and an increase in Succinivibrionaceae [20].

Antibiotics
Antibiotic administration has a significant effect on the microbiome. Improper use can lead to antibiotic-induced colitis, the overgrowth of pathogens such as Clostridioides, and an overall decrease in microbiome richness and diversity [24]. Different drugs have different effects on specific groups of bacteria [24]. Maintaining a diverse microbiota is important because species redundancy increases resilience, so if one species is harmed, the overall function of the system will be less impacted [24].

Although the microbiota largely returns to normal by a month post-treatment, some species may be affected for longer [24]. Other drugs that may impact microbiome composition include NSAID's, helminths, and anthelmintics [24]. The NSAID's phenylbutazone and firocoxib have been demonstrated to decrease concentrations of Firmicutes, Clostridiaceae, Ruminococcaceae, and Lachnospiraceae [24].

Foal Heat Diarrhea

Foals between 4 and 14 days are at an increased risk for developing mild diarrhea [22]. Foals with a decreased microbiome diversity (specifically decreased lachnospiraceae and ruminococcaceae) are at a higher risk [2]. In 7 day old foals with diarrhea, increased Enterobacteriaceae and Alcaligenceae concentrations have been found [10]. Additionally, higher levels of lactose digesting Bifidobacteriaceae were found in these foals in comparison to their older counterparts, likely due to their milk consumption at this age [10]. Heathy 7 day old foals, in comparison, had increased Actinomycetales, specifically in the family Micrococcaceae [10]. The presence of some Actinobacteria may aid in the maintenance of a healthy gut they aid in immune response to pathogens [14].

Septicemia

Sepsis is a disease that occurs when the body improperly reacts to infection. Septic shock causes organ failure due to lack of blood flow and tissue oxygenation. In foals, known causes include failure to transfer immunoglobulins from colostrum [3], issues with gestation or birth, and/or environmental contamination [4]. It can take up to four weeks of intensive care to recover from sepsis, but the recovery rate is still between 50-81% [5]. Sepsis may be in part due to translocation of bacteria from the gut to the bloodstream, which is at increased risk in the first 24 hours of life [4].

Healthy foals have been found to harbor greater numbers of Enterococcus (Firmicutes) and Pasteurellaceae (Proteobacteria), while septic foals had more Lactobacillus (Firmicutes), Facklamia (Firmicutes), and bacteria of the family Sphingobactarieaceae (Bacteroidetes) [4].

Colitis

Colitis is a blanket term for various diseases including clostridial enterocolitis, salmonellosis, and Aeromonas colitis [7]. These bacteria can be difficult to culture and present similarly, so they are generally classified under colitis. The disease is characterized by acute or long-term inflammation of the gut mucosa in the large bowel (cecum, colon) and presents as a sudden onset of watery diarrhea that causes dehydration and often rapid death [2,7]. A general decrease in microbial diversity, specifically in Firmicutes and Lactobacillus have been observed, but some conflicting reports do exist [24].
Colitis cases can also be triggered by:

- Bacterial infections (including Salmonella, Clostridioides Difficile, Clostridium Perfringens, and Neorickettsia Risticii) [2,24]
- Equine Coronavius [24]
- Parasite infections [2]
- Antimicrobial treatment (including Penicillin, Cephalosporins, Fluoroquinolones, and Trimethoprim-sulfadiazine) [2]
- Stress [7]

Colic

GI issues and stomach pain in horses are collectively known as colic. Colic cases range from mild to severe, but overall are a serious concern for horse-owners as there is only a 63% survival rate [2]. Horses colic easily because gas and fluid can only move through the digestive tract in one direction [9]. Thus, conditions which upset the stomach or flow of material can cause result in inflammation, severe pain, and even stomach rupture [9].

Causes of colic include:
- Sand ingestion [2]
- Stress [2]
- Changes in feeding, which can cause a rapid change in microbiome composition [2]
- Inflammation of the intestine, including that cause by enteritis or colitis [9]
- Inflammation of the abdominal cavity lining, including that caused by peritonitis [9]

Under normal circumstances, the equine GI tract's mucosal barrier prevents enteric bacteria and associated endotoxins from leaving the intestinal lumen, where they exist in high quantities [9]. In colic cases, disruption to the mucosal lining caused by inflammation or damage can allow bacteria and associated components to migrate to the perinatal cavity, from which they can enter the bloodstream [9]. Once circulating, these components, specifically flagellin and endotoxins become targets of leukocytes, which initiate inflammatory responses [9]. This can include abnormal coagulation, hypotension, reduced blood flow, depression, and fever [9]. Collectively, this response is known as endotoxemia, and treating it is vital [9]. Several solutions exist, including treatment with medication, antibodies, and even Polymyxin B, which at low quantities binds endotoxins without causing toxic effects itself [9].

Laminitis

Laminitis (also known as founder) is a disease in which the supporting tissue connected to the coffin bone (the lowermost bone in a horse's hoof) fails to secure to the hoof wall which can result in the bone twisting or dropping, sometimes completely through the bottom of the hoof. This is incredibly painful, causes severe lameness, and is incurable in severe cases. There are several causes of laminitis, one being sepsis associated [6]. Diseases causing sepsis that have also been associated with laminitis are usually caused by gram negative bacteria [6]. Increased Verrucomicrobia have been associated with chronic laminitis [21]. A high starch diet can cause an increase of bacteria that produce high levels of lactic acid, such as Streptococcus species [2,24]. This changed the gut pH, allowing more lactic acid-producing species to grow, furthering dysbiosis [24]. These changes can cause lactic acidosis which can lead to laminitis [2]. Other causes of sepsis related laminitis include retained fetal membranes, colic, and enterocolitis [6]

Peritonitis
Peritonitis is a condition that occurs in horses when the inner lining of the abdominal cavity, called the peritoneum, becomes inflamed [23]. It has several causes, including infection, which spreads via the bloodstream [23]. With GI tract perforation, a mixed population of bacteria are usually found at the infection site, but other tissue perforation and infection are more often associated with E. coli, Streptococcus, Staphylococcus, Klebsiella, Salmonella, Enterobacter, or Pseudomonas, to name a few [23]. The condition is treated with antimicrobials [23].

Anesthesia

Anesthesia is another documented cause of sudden microbiome change, and can further cause enrichment of [2]:
- Anaerostipes
- Ethanoligenens
- Enterococcus (firmicutes)
- Ruminococcus (firmicutes)

Other Diseases
Asthma: The occurrence of equine asthma may be associated with dysbiosis, as the microbial adaptation of the gut has been shown to differ between horses with and without asthma [24].

Equine Metabolic Syndrome: The microbiota composition of horses diagnosed with metabolic syndrome has been demonstrated to differ from healthy animals, suggesting a microbial role in the disease [24].

Obesity: It has been suggested that the gut microbiome may play a role in equine obesity via production of inflammatory cytokines [24].

Conclusion

Due to its observed role in maintaining beneficial microbial species in the gut, a fiber-based diet seems to be optimal for maintaining equine health [24]. something about many issues being connected?

References

Authored for BIOL 238 Microbiology, taught by Joan Slonczewski,at Kenyon College,2024