Actinobacillus actinomycetemcomitans: Difference between revisions
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==Genome structure== | ==Genome structure== | ||
The entire DNA genome of the Actinobacillus actinomycetemcomitans bacteriophage AaΦ23 was sequenced by using the shotgun sequencing. Linear DNA contained in the phage particles is circularly mixed and abundant in the end. Therefore, the entire DNA genome structure is circular. Its size is 43,033 bp with an overall molar G+C content of 42.5 mol%. Sixty-six potential open reading frames (ORFs) were found. This includes an ORF resulting from a translational frameshift, meaning if a ribosome changes frame when translating the genetic code. 23 of ORFs have a putative function. Twenty-three other ORFs are homologous with other bacteria. 20 ORFs came out to be specific to the phage AaΦ23. The organization of the phage genome and several genetic functions share extensive similarities with lambdoid phages (a large group of phages). However, AaΦ23 encodes a DNA adenine methylase, and the DNA packaging strategy is more closely related to the P22 system. The attachment sites of AaΦ23 (attP) and several A. actinomycetemcomitans hosts (attB) are 49 bp long. | The entire DNA genome of the Actinobacillus actinomycetemcomitans bacteriophage AaΦ23 was sequenced by using the shotgun sequencing (6). Linear DNA contained in the phage particles is circularly mixed and abundant in the end. Therefore, the entire DNA genome structure is circular. Its size is 43,033 bp with an overall molar G+C content of 42.5 mol%. Sixty-six potential open reading frames (ORFs) were found. This includes an ORF resulting from a translational frameshift, meaning if a ribosome changes frame when translating the genetic code (5). 23 of ORFs have a putative function. Twenty-three other ORFs are homologous with other bacteria. 20 ORFs came out to be specific to the phage AaΦ23. The organization of the phage genome and several genetic functions share extensive similarities with lambdoid phages (a large group of phages). However, AaΦ23 encodes a DNA adenine methylase, and the DNA packaging strategy is more closely related to the P22 system. The attachment sites of AaΦ23 (attP) and several A. actinomycetemcomitans hosts (attB) are 49 bp long (6). | ||
==Cell structure and metabolism== | ==Cell structure and metabolism== | ||
Revision as of 03:18, 27 August 2007
A Microbial Biorealm page on the genus Actinobacillus actinomycetemcomitans
Classification
Higher order taxa
Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Aggregatibacter; Aggregatibacter actinomycetemcomitans
Species
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NCBI: Taxonomy |
Actinobacillus actinomycetemcomitans
Description and significance
Actinobacillus actinomycetemcomitans is one of the most completely studied periodontal bacteria. It stays in the periodontal pocket of the oral cavity and damages tooth supporting tissues. See Pathology section for more details (4).
Actinobacillus actinomycetemcomitans is a gram negative bacterium which is spherical or rod-shaped (2). It’s a facultative anaerobe which can grow under either aerobic or anaerobic conditions (1). Actinobacillus actinomycetemcomitans is a typical cause of periodontitis but it may also be related to systemic infections and arterial plaques. Isolated Actinobacillus actinomycetemcomitans from periodontitis patients releases leukotoxin which kills T cells by some pathways (3). This will be discussed later in Pathology section.
A complete genomic sequence of Actinobacillus actinomycetemcomitans is available in publication by David Dyer, Bruce Roe and colleagues at the University of Oklahoma.
Genome structure
The entire DNA genome of the Actinobacillus actinomycetemcomitans bacteriophage AaΦ23 was sequenced by using the shotgun sequencing (6). Linear DNA contained in the phage particles is circularly mixed and abundant in the end. Therefore, the entire DNA genome structure is circular. Its size is 43,033 bp with an overall molar G+C content of 42.5 mol%. Sixty-six potential open reading frames (ORFs) were found. This includes an ORF resulting from a translational frameshift, meaning if a ribosome changes frame when translating the genetic code (5). 23 of ORFs have a putative function. Twenty-three other ORFs are homologous with other bacteria. 20 ORFs came out to be specific to the phage AaΦ23. The organization of the phage genome and several genetic functions share extensive similarities with lambdoid phages (a large group of phages). However, AaΦ23 encodes a DNA adenine methylase, and the DNA packaging strategy is more closely related to the P22 system. The attachment sites of AaΦ23 (attP) and several A. actinomycetemcomitans hosts (attB) are 49 bp long (6).
Cell structure and metabolism
Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.
Ecology
Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.
Pathology
How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
Application to Biotechnology
Does this organism produce any useful compounds or enzymes? What are they and how are they used?
Current Research
Enter summaries of the most recent research here--at least three required
References
Edited by student of Rachel Larsen
