Methanococcus voltae: Difference between revisions
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==Pathology== | ==Pathology== | ||
M. voltae has not been identified as a pathogen (3, 16). | |||
==Application to Biotechnology== | ==Application to Biotechnology== | ||
Revision as of 11:45, 31 August 2007
A Microbial Biorealm page on the genus Methanococcus voltae
Classification
Higher order taxa
Domain: Archaea
Phylum: Euryarchaeota
Class: Methanococci
Order: Methanococcales
Family: Methanococcaceae
Genus: Methanococcus
Species
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NCBI: Taxonomy |
Genus species: Methanococcus voltae
Other Name: Methanococcus voltaei
Strain: Methanococcus voltae PS
Description and significance
Methanococcus voltae is a single-celled, coccoid-shaped organism that is a member of the kingdom Archaea (2) . It belongs to a specific group called methanogens, or methane producers (3). Archaea thrive under extreme environmental conditions. Methanogenic archaeobacterium occur in anaerobic environments, such as the intestinal tracts of animals, freshwater and marine sediments, and sewage. They are capable of producing methane from a limited number of substrates, including carbon dioxide and hydrogen, acetate, and methylamines: an important source of natural gas (4). M. voltae’s main pathway for energy production is through methanogenesis (5). Archaea are similar to other prokaryotes in most aspects of cell structure but are unique with respect to the lipid composition of the cytoplasmic membrane and the structure of the cell surface (6). Most archaeal species have walls made of proteins or glycoproteins, which form the outermost envelope of the cell called the surface layer (S-layer), while other species’ membranes are composed of phospholipids (7, 6). The unique cell surface of archaea requires distinct solutions to the problem of how proteins cross this barrier to be either secreted into the medium or assembled as appendages at the cell surface (6).
Genome structure
Several genes for chromatin proteins are known in Archaea. Some include histones and histone-like proteins in Euryarchaeota, as well as a DNA binding protein, Alba, which was first detected in the crenarchaeote Sulfolobus solfataricus and is thought to be involved in transcriptional regulation (8). M. voltae harbors four genes coding for all these types of chromatin proteins (8). M. voltae’s genomic structure and adaptations provide insight into understanding the organism’s interactions with its extreme habitat. “Methanococcus voltae (strain PS) is known to produce a filterable, DNase-resistant agent (called VTA, for voltae transfer agent), which carries very small fragments (4400 bp) of bacterial DNA and is able to transduce bacterial genes between derivatives of the strain” (9). M. voltae was found to have a system of gene transfer similar to general transduction except that the bacteriophage component (in terms of virus replication) is defective or absent (9). VTA is responsible for the transfer and its 4.4kb fragments of DNA are resistant to DNase.
Cell structure and metabolism
M. voltae is arranged in singles and pairs, is motile, aquatic, anaerobic, and has a mesophilic temperature range (1). Its head diameter is about 40nm, and tail length is about 61nm (10). It is a methanogen, has a carbon cycle, and is nonsporulating (16). M. voltae also has its DNA packaged at about 4.4kb, with a size of 2000kb (6, 7). M. voltae lacks a typical bacterial cell wall. Only a thin protein S-layer covers the plasma membrane (3, 10). The lack of a cell wall explains why Archaea are resistant to antibacterial antibiotics, which are often used as genetic markers (3). Its source of mobility is via utilization of its flagella. The flagella of M. voltae are predicted, from their gene sequences, to be about 22 to 25 kDa (2). Archaea have lipids with links between the head group and side chains, and makes the lipids more resistant to heat and acidity than bacterial and eukaryotic ester-linked lipids. The glycerol headgroup with two ether-linked side-chains is known as archaeol (9). During methanogenesis, M. voltae undergoes a process during which hydrogen is used as an energy source to reduce carbon dioxide to methane (5). This species will produce methane by undergoing anaerobic metabolic processes. Hydrogen gas is used as an electron reductant (or electron donor) for carbon dioxide reduction into methane (13).
Ecology
Methanogens are extremely important to the anaerobic environments in which they live because they convert organic compounds into methane, which then rise into the aerobic environment. Through this process, these organisms provide a pathway for compounds that exist in anaerobic environments to escape into the atmosphere, thus acting as a natural gas resource (14). M. voltae needs selenium for optimal growth and energy production (12). High concentrations of selenium are toxic for most organisms, and detoxification can be achieved by volatilization through methylation (12). Dimethylselenide is one product of methylation and has been monitored in soil and marine environments where M. voltae live (12). Archaea may have a great ecological impact with the production of methane. As methane is a powerful greenhouse gas, archaea may be partly responsible for global warming.
Pathology
M. voltae has not been identified as a pathogen (3, 16).
Application to Biotechnology
Does this organism produce any useful compounds or enzymes? What are they and how are they used?
Current Research
Enter summaries of the most recent research here--at least three required
References
(1) "Methanococcus voltae". NCBI Taxonomy Browser. 27 August 2007. [1]
(2) Bayley, DP. & Jarrell (1999) Overexpression of Methanococcus voltae Flagellin Subunits in Escherichia coli and Pseudomonas aeruginosa: a Source of Archaeal Preflagellin. American Society for Microbiology, 181(14): 4146–4153. Link to Article
(3) Tumbula, D., and Whitman. W., “Genetics of Methanococcus: possibilities for functional genomics in Archaea.” Molecular Microbiology 1999. Volume 33: 1-7.
(4) "Methanococcus voltae" Dictionary Reference. 27 Aug 2007. Link to Website
(5) Zhu W, Reich CI, Olsen GJ, Giometti CS, Yates JR 3rd., J. Proteome Res. 2004. Volume 3: 538-548.
(6) Albers, SV. (2006). Protein secretion in the Archaea: multiple paths towards a unique cell surface. Nature reviews. Microbiology, 4(7), 537-547. Link to Article
(7) Sleytr, UB., Egelseer, E., Ilk, N., Pum, D., and Schuster, B., “S-Layers as a basic building block in a molecular construction kit.” 2007. Volume 274(2): 323-334.
(8) Heinicke, I. (2004). Mutational analysis of genes encoding chromatin proteins in the archaeon Methanococcus voltae indicates their involvement in the regulation of gene expression. Molecular genetics and genomics, 272(1), 76-87. Link to Article
(9) Ng, SYM, & Ng. (2007). Archaeal signal peptidases. Microbiology, 153(2), 305-314. Link to Article
(10) Lang, AS, & LANG. (2007). Importance of widespread gene transfer agent genes in alpha-proteobacteria. Trends in Microbiology, 15(2), 54-62. Link to Article
(11) "Methanococcus voltae" 27 Aug 2007. Link to Website
(12) Niess, UM. (2004). Dimethylselenide demethylation is an adaptive response to selenium deprivation in the archaeon Methanococcus voltae. Journal of bacteriology, 186(11), 3640-3648. Link to Article
(13) Dawes, Edwin. Microbial Energetics. New York: Blackie. 1986
(14) Reeve, J.N. “Molecular biology of Methanogens.” Annu Rev Microbiol. 1992. Volume 46: 165–191.
(15) Allers, T. (2005). Archaeal genetics - The third way. Nature reviews. Genetics, 6(1), 58-73. Link to Article
(16) "Methanococcus voltae" Genamics GenomeSeek. 27 Aug 2007. Link to Website
Edited by student of Rachel Larsen
