C. trachomatis: Difference between revisions
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==Genome Structure== | ==Genome Structure== | ||
Chlamydia trachomatis has a genome that comprises of 1,042,519 nucleotide base pairs and has roughly 894 likely protein coding sequences. [2] C. trachomatis strains have an extrachromosomal plasmid, which was sequenced to be a 7493-base pair plasmid. Because there is a smaller amount than 1% nucleotide sequence variation, ll plasmids from human C. trachomatis isolates are reflected to be very comparable. All the isolates "are about 7,500 nucleotides long and has eight open reading frames computer-predicted to code for proteins comprised of more than 100 amino acids, along with short non-coding sequences amongst some of them." [1] Stimulatingly, in their nucleotide sequence, chlamydial plasmids are extra closely related than is the matching chromosomal DNA. The plasmid of C. trachomatis is a likely target for DNA-based diagnosis of C. trachomatis simply because there are give or take 7-10 copies of the plasmid existing per chlamydial particle. Its sequence is exceedingly conserved among different isolates of C. trachomatis. Some C. trachomatis strains are absent in these plasmids, and the concerns aid in recognition of the C. trachomatis strain. Plaque purified C. trachomatis that do not comprise the plasmids have uncommon inclusion morphology, have no glycogen, and show no change in antibiotic sensitivity. Nevertheless, the fact that such strains are present displays that the plasmid is not a must for C. trachomatis survival [1]. | |||
==Cell Structure, Metabolism and Life Cycle== | ==Cell Structure, Metabolism and Life Cycle== | ||
Revision as of 03:51, 19 April 2011
Classification
Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]
Species
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NCBI: Taxonomy |
Genus species
Description and Significance
Describe the appearance, habitat, etc. of the organism, and why you think it is important.
Genome Structure
Chlamydia trachomatis has a genome that comprises of 1,042,519 nucleotide base pairs and has roughly 894 likely protein coding sequences. [2] C. trachomatis strains have an extrachromosomal plasmid, which was sequenced to be a 7493-base pair plasmid. Because there is a smaller amount than 1% nucleotide sequence variation, ll plasmids from human C. trachomatis isolates are reflected to be very comparable. All the isolates "are about 7,500 nucleotides long and has eight open reading frames computer-predicted to code for proteins comprised of more than 100 amino acids, along with short non-coding sequences amongst some of them." [1] Stimulatingly, in their nucleotide sequence, chlamydial plasmids are extra closely related than is the matching chromosomal DNA. The plasmid of C. trachomatis is a likely target for DNA-based diagnosis of C. trachomatis simply because there are give or take 7-10 copies of the plasmid existing per chlamydial particle. Its sequence is exceedingly conserved among different isolates of C. trachomatis. Some C. trachomatis strains are absent in these plasmids, and the concerns aid in recognition of the C. trachomatis strain. Plaque purified C. trachomatis that do not comprise the plasmids have uncommon inclusion morphology, have no glycogen, and show no change in antibiotic sensitivity. Nevertheless, the fact that such strains are present displays that the plasmid is not a must for C. trachomatis survival [1].
Cell Structure, Metabolism and Life Cycle
The life phase of Chlamydia trachomatis comprises of two steps: elementary body and reticulate body. The elementary body is the spreading form, which is analogous to a spore. The spreading form is about 0.3 um in diameter and makes its own endocytosis upon contact to target cells. It is this form that averts phagolysosomal fusion, which then permits for intracellular survival of the bacteria. Once inside the endosome, the elementary body develops into the reticulate body as a result of the glycogen that is created. The reticulate body splits through binary fission at approximately 2-3 hours per generation. The cell body has a maturation period of 7-21 days in the host. It has no cell wall and is identified as an inclusion in the cell. After division, the reticulate body converts back to the elementary form and is released by the cell by exocytosis. One phagolysosome generally make bout 100-1000 elementary bodies [2]. For metabolism, Chlamydia trachomatis has a glycolytic pathway and a linked tricarboxylic acid cycle. Glycogen production and use of glucose derivatives plays a supportive role in chlamydial metabolism. The occurrence of metabloic precursors and products, such as pyruvate, succinate, glycerol-3-phosphate and NADH dehydrogenases, NADH-ubiquinone oxidoreductase and cytochrome oxidase specify that Chlamydia trachomatis uses a form of electron transport in order to yield energy [2].
Ecology and Pathogenesis
Chlamydia trachomatis is a pathogenic bacteria. It cannot stay alive outside of a eukaryotic host. In fact, humans are the only recognized usual host for C. trachomatis. The bacterium is transmitted by sexual contact with an infected individual.[3] Usually, C. trachomatis is asymptomatic in its hosts, but can produce discharge from the penis, pain and burning through urination, infection or inflammation in the ducts of testicles, and sensitivity or pain in the testicles. [3]
References
Author
Page authored by Alicia Smith, student of Prof. Doreen Cunningham at Saint Augustine's College.
