Mycobacterium smegmatis
A Microbial Biorealm page on the genus Mycobacterium smegmatis
Classification
Higher order taxa
Bacteria (Domain); Actinobacteria (Phylum); Actinobacteridae (Class); Actinomycetales (Order); Corynebacterineae (Suborder); Mycobacteriaceae(Family); Mycobacterium (Genus)
Species
Mycobacterium smegmatis.
Also known by: Mycobacterium paratuberculosis smegmatis, Bacterium smegmatis, Bacillus smegmatis, Mycobacterium paratuberculosis smegmatis, Bacterium smegmatis, Bacillus smegmatis, Mycobacterium smegmatis.
Description and significance
Mycobacterium smegmatis was first discovered in 1884 by Lustgarten. They are mostly found in the soil, marine, and freshwater environments. Mycobacterium smegmatis is classified as a saprophytic species that rarely causes disease and isn't dependent on living in an animal, unlike some pathogenic Mycobacterium. It is a very important bacteria because it is very useful for studying cultures of Mycobacterium. There are several Mycobacterium species that are harmful and are common diseases, like Mycobacterium leprea, Mycobacterium tuberculosis, and Mycobacterium bovis. Mycobacterium smegmatis is so important because it is fast growing and non-pathogenic compared to these species. Currently, there are many laboratories that are culturing this species to determine the pathological course of deleterious Mycobacteria.
Describe the appearance, habitat, etc. of the organism, Describe how and where it was isolated. Include a picture or two (with sources) if you can find them.
Bacillus licheniformis is a bacterium that is commonly found in soil and bird feathers. Birds that tend to stay on the ground more than the air (i.e. sparrows) and on the water (i.e. ducks) are common carriers of this bacterium; it is mostly found around the bird's chest area and back plumage.
Bacillus licheniformis is part of the subtilis group along with Bacillus subtilis and Bacillus pumilus. These bacteria are commonly known to cause food poisoning and food spoilage. Bacillus licheniformis also is known for contaminating dairy products. Food borne outbreaks usually involve cases of cooked meats and vegetables, raw milk, and industrially produced baby food contaminated with Bacillus licheniformis.
Genome structure
The genome is 6,988,209 nucleotides long. It has a 67% GC content and a 33% AT content, and is therefore classified as a high GC content gram positive bacteria (discussed below). 90% of the genome represents coding regions that encode for 6718 proteins. There are 6938 genes that are composed circularly with an absence of any plasmids. Mycobacterium smegmatis is a slow growing bacteria which contains one copy of the ribosomal RNA genes unlike fast growing bacteria (Eg. Escherichia coli) which has two copies of the rRNA genes. Mycobacterium smegmatis doesn't need so many copies of the genes because it doesn't require the high production of proteins when it is growing slow, while Escherichia coli does.
Other interesting features? What is known about its sequence?
Cell structure and metabolism
Mycobacterium smegmatis is a Gram-positive bacteria, characterized by an inner cell membrane and a thick cell wall. The Gram-positive bacteria is further classified as one with a high GC content and therefore a low AT content. This quality is used as a rude measure of similarity of different species of bacteria. Although this bacteria is Gram-positive, its cell wall contains mycolic acids, long, branched fatty acids that are normally present in Acid-fast bacteria. The acids prevent proper gram staining that would normally identify the cell as a gram positive cell because they create a waxy coating so the crystal violet has difficulty entering the cell, therefore making it seem gram negative. The cell wall is also abnormal because it is irregularly thick for a gram positive bacteria and its hydrophobicity reduces dessication. This feature in addition to its slow cell growth attribute to Mycobacterium smegmatis' low response to antibiotics.
Although it contains the similar structural features of Mycobacterium Tuberculosis, it grows much quicker in comparison. Mycobacterium smegmatis is an aerobic organism. Small amounts of Mycobacterium smegmatis may also survive on chemolithoautotrophic growth. They would use carbon dioxide and hydrogen gas as its inorganic fuel source. It requires a unique fatty acid biosynthesis to produce the mycolic acids that are present on the cell wall.
what important molecules it produces.
Ecology
Mycobacterium smegmatis lives in aggregate layers of cells attached to each other in a community called a biofilm. may live in soil and degrade organic materials, including sterols. The bacteria lives in water, and tends to exist near large bodies of water.
Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.
Pathology
This organism is saprophytic and therefore extremely unpathogenic. Mycobacterium smegmatis doesn't reside in any animals, and doesn't cause dangerous or even any infections. There are no threats as it is very safe. However other species under this genus are pathogenic. Obligate pathogens such as Mycobacterium tuberculosis, Mycobacterium bovis, and Mycobacterium leprea are highly pathogenic in animals. These species can cause tuberculosis and leprosy. There is also the classification of potential pathogens in this genus, such as Mycobacterium avium.
Application to Biotechnology
Does this organism produce any useful compounds or enzymes? What are they and how are they used?
Current Research
Mycobacterium smegmatis doesn't have a lot of research performed to discover more about the genus' different functions that can translate into ways to treat pathogenic Mycobacterium, like Mycobacterium tuberculosis and leprea.
Dr. Fahey's lab at UCSD studies the methods of mycothiol biosynthesis that produces the thiols that are needed for Mycobacterium to live and grow. The lab is trying to determine the missing and unknown enzymes and substrates that are present in the biosynthesis and if they are essential. There are currently three steps that have been determined by this lab. They are trying to determine the unknown substrate that is converted to GlcNAc-Ins from the enzyme MshA. From 3 more known steps using MshB, MshC, and MshD, the necessary mycothiol is produced. The Fahey lab aims to determine the unknown substrate to determine the full mycothiol biosynthesis pathway, and to determine some inhibitors of this pathway to prevent Mycobacterium smegmatis growth, which can also translate to treatments for Mycobacterium tuberculosis. 2
Mycobacterium smegmatis uses a terminal oxidase to donate electrons to the final electron acceptor oxygen in oxidative phosphorylation during aerobic respiration. These terminal oxidases have been identified as using both a cytochrome c aa3 type oxidase and a quinol bd type oxidase. When bd type oxidase gene is knocked out to only allow for the cytochrome c aa3 type oxidase to function, the latter didn't attain normal levels of expression of the oxidase. In addition, the oxidase concentration for the knockout didn't reduce during log phase, while the wild type(without the knockout) had decreased oxidase concentration. 3
Enter summaries of the most recent research here--at least three required
References
1. Dastur, A., Kumar, P., Ramesh, S., Vasanthakrishna, M., Varshney, U. "Analysis of the initiator tRNA genes from a slow- and a fast-growing Mycobacterium" Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560 012, India. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12209262&dopt=Abstract
2. Newton, G., Ta, P., Bzymek, K., Fahey, R. "Biochemisrty of the Initial Steps of Mycothiol Biosynthesis" Journal of Biological Chemistry. 2006. Volume 281. p. 33910-20.
3. Megehee, L. "Temporal expression of Mycobacterium smegmatis respiratory terminal oxidases" Canadian Journal of Microbiology. 2007. Volume 53. p. 459-6.
Edited by Benjamin Yip, student of Rachel Larsen and Kit Pogliano
