Pseudoxanthomonas winnipegensis

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Classification

Domain;Bacteria Phylum;Pseudomonadota Class;Gammaproteobacteria Order;Xanthomonadales Family; Xanthomonadaceae


Species

NCBI: [1]


Pseudoxanthomonas

Description and Significance

Pseudoxanthomonas winnipegensis is a gram negative bacteria. This species obtains the bacillus shape and has a yellowish pigment. Pseudoxanthomonas winnipegensis was derived water, plants or contaminated soils. Recent studies recovered the bacteria from human clinical materials and was recently recovered from cystic fibrosis patients. Until recently, Pseudoxanthomonas species were only identifiable at the genus level. New gene sequencing improvements have allowed for the identification of species within the genus.

Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence?

Specific strains of this bacteria were studied by whole genome sequencing which used average nucleotide identity, DNA-DNA hybridization, amino acid identity, core genome and nucleotide analyses, biochemical and cellular fatty acid analysis and antimicrobial susceptibility testing. Pseudoxanthomonas winnipegenesis structure was investigated using scanning microscopes and transmission electron microscopes. The bacteria was found to be a gram-negative bacilli.

Bacteria were studied by whole genome sequencing using average nucleotide identity by Blastn, digital DNA-DNA hybridization, average amino acid identity, core genome and single nucleotide variant analyses, MALDI-TOF, biochemical and cellular fatty acid analyses, and by antimicrobial susceptibility testing. Bacterial structures were assessed using scanning and transmission electron microscopy. Strains were strict aerobes, yellowish-pigmented, oxidative, non-motile, Gram-stain-negative bacilli and generally unable to reduce nitrate. Strains were susceptible to most of the antibiotics tested; some resistance was observed towards carbapenems, several cephems and uniformly to nitrofurantoin. The single taxon group observed by 16S rRNA gene sequencing was supported by whole genome sequencing; genomes ranged in size from 4.36 to 4.73 Mb and had an average G+C content of 69.12 mol%

Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.


Ecology and Pathogenesis

Habitat; symbiosis; biogeochemical significance; contributions to environment.

If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.


References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.


Author

Page authored by Bella Manfredi, student of Prof. Bradley Tolar at UNC Wilmington.