Kyasanur Forest Disease Virus
Classification
Flaviviridae; flavivirus
Description and significance
Kyasanur forest disease (KFD) is a tick-borne viral hemorrhagic fever (Murhekar et. al., 2015) which was first discovered in 1956, in the forests of southern India where it is endemic. Originally indigenous to the Shimoga district in Karnataka state, it has gradually emerged in other parts of southern Asia causing epidemics in recent decades (Mourya et. al., 2012; Nichter 1987). A major factor in its initial emergence was deforestation which brought monkeys into close contact with humans (Gould et. al,. 2008). The virus (KFDV) was first identified in sick monkeys inhabiting the Kyasanur forest during a series of epizootic outbreaks. Although many animal species appear to be resistant to infection by this virus, the black-faced langur (Presbytis entellus), the red-faced bonnet monkey (Macaca radiata) and humans are highly susceptible to infection by KFDV. Haemaphysalis spinigera ticks act as vectors for the virus. These ticks have a life cycle lasting approximately 12 months and involving three feeding stages. At each stage of this cycle they take a bloodmeal from mammals which if infected will transfer the virus to the feeding tick.When replete, ticks drop off the host animal landing in the moist undergrowth where they remain for several months undergoing transstadial changes to the next stage of their life cycle (from larvae, to nymphs and then to adults). When these infected ticks take their next bloodmeal, they may infect the host on which they feed. Humans with occupational exposure to forest animals (i.e., herders, farmers, forest workers, hunters) are especially at high risk of infection when they come into contact with the ticks on the animals or in the undergrowth. Consequently, KFD has become a concern for public health authorities within areas endemic for KFDVl(Mehla et. al., 2009).
Current studies of KFDV are focused on improvement of disease control strategies and molecular epidemiological analyses to track the dispersal of KFDV and close relatives outside of India. Methods for disease control include, i) understanding the modes of virus transmission, ii) developing effective rapid diagnostic tests, iii) improving the safety and effectiveness of available vaccines with which to protect humans from infection against KFD, and antiviral drug discovery to provide post-infection treatment (Mourya et. al., 2012; Kasabi et. al., 2013; Murhekar et al., 2015; Mehla et. al., 2009). Moreover, improved molecular methods for virus identification and characterisation have now demonstrated the presence of KFDV and the closely related Alkhurma hemorrhagic fever virus (AHFV) in Saudi Arabia (Mehla et. al., 2009), China (Wang, 2009) and Egypt (Charrel and Gould, 2011), suggesting that KFDV has its evolutionary origins in Africa (Charrel and Gould, 2011).
Despite current efforts to control and trace the movement of KFDV, particularly in India where the virus was originally believed to have its evolutionary origins, it is clear that this virus and its close relatives are causing increasing numbers of cases of hemorrhagic fever and in some cases encephalitis (Pattinaik, 2006). Indeed, with increasing global temperatures, increasing transportation of animals, humans and commercial goods across the world, the need to develop strategies to control the spread of these deadly pathogens and many related viruses is a growing challenge (Gould et. al., 2008).
Genome structure
As part of the family Flaviviridae, this virus contains a spherical positive single-stranded RNA molecule of 11,000 bases in length (Chambers et al. 1990; Pattnaik, 2006; Heinz & Stiasny, 2012).
Morphology
KFDV is pathogenic member of tick-borne encephalitis virus group (TBEV). This group consists of related viruses such as the Louping ill, Omsk hemorrhagic fever, Langat, Royal Farm, Gadgets Gully, and Powassan viruses (Mehla et. al., 2009). There is a wide range of tick species contributing to the tick-borne KFDV group, mainly the Haemaphysalis spinigera. Mature TBEVs have a diameter of 50 nm and contain 3 structural proteins for the capsid, envelope, and membrane (Heinz & Stiasny, 2012). These viruses enter the cell via receptor-mediated endocytosis and undergo an immature and mature phase inside the cell (Heinz & Stiasny, 2012). A recent study showed that infection of KFDV is lethal in mice and leads to elevated levels of pro-inflammatory cytokines in the brain. However, no viruses were detected in visceral organs (Sawatsky et. al., 2014).
Ecology
Other than Haemaphysalis spinigera, KFDV has been isolated from 16 different types of ticks. Wild monkeys, Presbytis entellus (Black faced langur) and Macaca radiate (Red faced bonnet monkey), are common in the forest localities that commonly get infected by KFDV through bites of infected ticks, acting as sentinel animals as they are susceptible to KFDV (Murhekar et al., 2015). KFDV also circulates in small animals such as rodents, shrews, and birds (Banerjee K., 1988). The ecology of the Shimoga area is characterized by high humidity and is special due to the presence of divergent biotypes of forests, cultivated areas, and grasslands. The high humidity generated from the cultivated fields is favorable for tick maintenance throughout the year and availability of nearby forest sustains a large population of wild monkeys (Boshel M-J., 1969). Because such ecological specificity is rarely observed in other parts of India, this combination of ecological factors may be responsible for the geographical localization of KFDV only to Karnataka state of India (Boshel M-J., 1969). Tick-borne viral hemorrhagic fever, however, is also present throughout other parts of Eurasia as the TBEV viral group undergoes recombination events, creating different viruses better adapted to and that are easily spread (Gould, 2009).
Transmission
Pathology and Diagnostics
How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
Vaccination and Prevention
Vaccination with formalin-inactivated tissue-culture vaccine has been the primary strategy for controlling KFD in the five endemic districts of Karnataka since 1990 (Murhekar et al., 2015). The strategy involves mass vaccination in areas reporting KFD activity (defined as laboratory evidence of confirmed cases in monkeys, humans, or ticks) and in villages within a 5-km radius of such areas. Two vaccine doses are administered at least 1 month apart to persons 7–65 years of age (Kiran et al., 2015). Vaccine-induced immunity is short-lived, so the first booster dose of vaccine is recommended within 6–9 months after primary vaccination; thereafter, annual booster doses are recommended for 5 years after the last confirmed case in the area (Kiran et al., 2015). Besides vaccination, other preventative measures include utilization of insect repellents and wearing protective clothing to avoid tick bites (Centers for Disease Control and Prevention [CDC] 2014).
People residing in the forest areas, as well as those working in the parks, sanctuaries, and reserve forests remain at risk of acquiring the disease. As the presence of the disease often becomes noticeable when enzootic infections occur and sentinel animals such as monkeys start dying, establishing an event-based surveillance system for monkey deaths in the national parks, wildlife sanctuaries, reserve forests of the Western Ghats, and neighboring villages may help detect the disease early to institute appropriate control measures (Murhekar et al., 2015). Conducting sero-surveys in different districts of the region is another possibly helpful measure for mapping of the disease (Murhekar et al., 2015).
Current Research in Related Viruses
Include information about how this microbe (or related microbes) are currently being studied and for what purpose
References
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Charrel, Remi, and Ernest A. Gould. Alkhurma Hemorrhagic Fever in Travelers Returning from Egypt, 2010. Emerg. Infect. Dis. Emerging Infectious Diseases (2011): n. pag. Web.
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Dodd, Kimberly, Brian Bird, Marina Khirstova, Cesar Albariño, Serena Carroll, James Corner, Bobbie Erickson, Pierre Rollin, and Stuart Nichol. Ancient Ancestry of KFDV and AHFV Revealed by Complete Genome Analyses of Viruses Isolated from Ticks and Mammalian Hosts. PLOS Neglected Tropical Diseases:. PLOS. Web. 03 Dec. 2015.
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Sawatsky, Bevan, Alexander J. Mcauley, Michael R. Holbrook, and Dennis A. Bente. Comparative Pathogenesis of Alkhumra Hemorrhagic Fever and Kyasanur Forest Disease Viruses in a Mouse Model. PLoS Negl Trop Dis PLoS Neglected Tropical Diseases. 2014 Jun; 8(6): e2934
Wang J, Zhang H, Fu S, Wang H, Ni D, Nasci R, et al. Isolation of Kyasanur forest disease virus from febrile patient, Yunnan, China. Emerg Infect Dis. 2009. 15(2): 326–8. doi:10.3201/eid1502.080979.
