Xanthomonas axonopodis

A Microbial Biorealm page on the genus Xanthomonas axonopodis

Classification

Higher order taxa

Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadacae; Xanthomonas

Species

NCBI: Taxonomy

Genus species testing

Description and significance

Infected plants have leaves and fruits with brown colored lesions surrounded by oily, water soaked yellow rings. These lesions may in time fall out creating a sharp hole in the leaves. This bacteria may be found in any or all different types of citrus. These include oranges, grapefruits, limes, lemons, etc.

Genome structure

Chromosome is circular (5,175,554 bp) as well as the plasmids (33,700 bp and 64,920 bp). This bacteria is gram negative and "produces slow growing, non-mucoid colonies in culture, ecologically obligate plant parasite".

Cell structure and metabolism

Flagella is present and interacts through plant pathogen. 2 membranes are present and no inteins.

Ecology

This bacteria occurs in agricultural and urban areas. It generally affects citrus trees in high temperature areas, heavy rainfalls, and high winds. Due to wind and rainfall, this bacteria may spread throughout situated areas. It is most likely to infect plants in high temperature and heavy rainfall weather. This causes citrus canker, which may progressively and ultimately lead to economic losses on citrus industries.

Pathology

This bacteria contaminates citrus plants that prevents progressive fruit and health production. It diminishes fruit production of trees, ultimately leading to no fruits and death of plant. This disease may contaminate plants and even equipments. It was originated in 1912 in Florida. It started to spread within Florida and only in Florida. Yet years later in Miami, a new form of this bacteria started to spread and ultimately causing a bigger and deadly problem.

Application to Biotechnology

Current Research

Citrus canker was concluded to be a threat to natural environment and economy. It has been tested and researched to produce a feasible elimination of this baterium. Yet on January 11, 2006, USDA has come to the conclusion that the eradication of this baterium is not feasible. Due to the unexpected hurricanes in the years 2004 to 2005, the top United States Department of Agriculture officials came to conclude that this disease has spread drastically and is difficult to eradicate. Because eradication of this baterium is not feasible, officials demand a new approach to decrease, not eliminate, the spread of citrus canker and inhibit further contamination of this bacteria.

For accurate identification of this bacterium, couple PCR methods were developed. A plasmid containing pthA gene was used as a primer for this testing. The A-strain was not able to accurately identify the A strain variant Aw(Wellington strain).

Rep-PCR with BOX and ERIC primers were used to isolate and identify the different canker strain types. They related these identifications with the spread of canker in Florida and Miami. Rep-PCR supported Aw strains among A strains to partake in the spread of this disease in Florida. Strains were isolated and compared with strains found in other geographic areas. Strains found in Manatee County, Florida matches the strains from China and Malaysia. The MI strain (Miami) matches several locations in south and central Florida. These comparisons furthered proved the conclusion and there was a movement of plant material that took place between these countries and counties that caused this disease to spread.