SungTimon2.2
Classification
Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]
Species
|
NCBI: Taxonomy |
Genus species
Habitat Information
Describe the location and conditions under which the organism was isolated.
Description and Significance
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.
Genome Structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.
Cell Structure, Metabolism and Life Cycle
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Physiology and Pathogenesis
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
Various Test Results:
Phenol Red Broth Test- Results [Lactose: Pinkish-Red, meaning slightly alkaline product] [Sucrose/Glucose: Yellow-Orange, meaning slightly acidic product] NO GAS (fermentation) produced
Stach Hydrolysis- Results [Negative, meaning our bacteria does not hydrolyze starch via the use of amylase]
Casein Hydrolysis- Results [Positive, meaning our bacteria possesses the enzyme casease in order to hydrolyze the milk protein casein]
Gelatin Hydrolysis- Results [Negative, meaning our bacteria does not hydrolyze gelatin via the use of gelatinase]
DNA Hydrolysis- Results [Positive, meaning our bacteria possesses the enzyme DNase in order to catalyze DNA]
Lipid Hydrolysis- Results [Positive, meaning our bacteria possesses the enzyme ligase in order to breakdown lipids]
Methyl Red-Voges Proskauer: Results [Negative, the organism does not utilize mixed acid fermentation] [Negative, the organism does not reneutralize, meaning the organism does not make acetoin]
Citrate Test- Results [The organism does not possess citrate permease, and cannot use citrate as the sole source of carbon]
SIM Test (3 in 1)- Results [Sulfur reduction: negative, Indole production for tryptophan: negative, motility: nonmotile]
Nitrate Reduction Test- Results [negative for nitrate reducing enzymes]
Urea Hydrolysis- Results [negative for the enzyme urease]
Triple Sugar Iron Agar- Results [positive for fermentable enzymes] **"orange butt, yellow slant... which we concluded that the butt was not stabbed far enough"
Oxidase- Results [positive for the enzyme cytochrome C oxidase]
References
Author
Page authored by Hsiang-Yuan Sung and Kaleen Timon, student of Prof. Kristine Hollingsworth at Austin Community College.
