Naegleria fowleri
A Microbial Biorealm page on the genus Naegleria fowleri
Classification
Higher order taxa
Eukaryota, Percoloza, Heterolobosea, Schizopyrenida, Vahlkampfiidae, Naegleria, fowleri
Species
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NCBI: Taxonomy |
Naegleria fowleri
Description and significance
Naegleria fowleri are found in warm bodies of water across the globe. Its abundance on the earth and its severely toxic result on human hosts are of concern in the medical field. N. fowleri is responsible for causing over 300 cases of primary amoebic meningoencephalitis, or PAM, of which there are only seven survivors though exact numbers of cases and survivors vary by report. Those who fall victim to an infection of N. fowleri will usually die within two weeks of the initial infection. Its ability to transform into a bi-flagellate stage has also increased interest in this microorganism and it's use as a model for differentiation of eukaryotic cells (G).
Genome structure
Few studies have been done involving the genome of Naegleria fowleri, though some important information has been discovered. Although it is known that N. fowleri is polyploidy,the exact number of genome copies is unknown (E). Its genome size was orginally estimated at over 70.6 kbp, obtained by summing the 16.5kpb ribosomal DNA and its 54.1kpb mitochondrial DNA (A). Both the rDNA and mtDNA sizes were calculated by restriction endonuclease fragments. Further research has discovered a 104 kbp genome with 23 chromosomes (G). The rDNA is kept on extrachomosonal, circular plasmids called episomes ranging in size from 14kpb to 17kbp. As the entire genome is polyploidy, these eposomes are multicopy episomes up to 4000 copies. Species classification is done using either 5.8s rDNA or SSUrDNA (G)
Life Cycle
Naegleria fowleri exist in three stages; cyst, amoebiod trophozoites and flagellated trophozoite. At less than ideal conditions (below 27oC), the amoeba forms a spherical cyst, approximately 7-14μm. In the cyst stage, the single nucleus is protected by a dense cell wall and a a very granular cytosol. The cell wall generally contains two pores which are plugged with mucus until trophozoite stage is induced (C). When the cyst is activated, binary fission occurs into the trophozoite stage. The trophozoite stage is the only stage in which N. fowleri can grow. Although infection can be caused by cysts in dust, the trophozoite stage is the major active and infectious stage of the life cycle (B). In this primary trophozoite stage, the cells are between 10-30 mu;m characterized by a dark, central nucleus (C). Many vacuoles are seen in the cytoplasm which aid in the cell's movement by lobopodia (D). An intermediate flagellate trophozoite stage is also observed in some, but not all strains of N. fowleri. These pear shaped, lophotichous cells use 2 flagella on one end of the cell for movement in finding a suitable host. This transformation into the flagellated stage can be induced by changing the environment of the bacteria, even as simply as being placed in distilled water (C).
Cell structure and metabolism
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Ecology
Naegleria fowleri are found world wide in warm freshwater, either naturally geothermic pools or warm bodies of water created by industry such as industrial cooling water (H). Although they ideally grow at 37oC, the temperature of their human hosts, below 27oC, N. fowleri are able to survive by encysting. The amoeba is able to grow in environments up to 45oC (D). Although it is found all across the globe, the only place where N. fowleri contaminates public drinking water is in South Australia (H). It has even been found in poorly chlorinated pools in southern regions of the United States and other warm locales. This widespread finding of N. fowleri and the low number of cases of N. fowleri related PAM lead to the belief that many more humans are exposed to N. fowleri than actually become infected.
Pathology
Although humans are a known host of N. fowleri, since infections are so rare, it is expected there is an alternative, primary host such as fish, amphibians or reptiles. N. fowleri are known parasitize fish and antibodies to different Naegleria species have been found in many wild animals (G). In humans, N. fowleri enters the olfactory mucosa through the nose either by splashing or diving into contaiminated waters (C). It crosses barriers and invades the subarachnoid space where it focuses on the olfactory bulb and nerves connected to the brain, causing an infection of the central nervous system. The resulting primary amoebic meningoencephalitis, or PAM, is generally fatal. The trophozotic stage is actively growing and diving, it's mitochondrial activity encouraged by the high oxygen concentration in the brain (C). Once in the brain, N. fowleri phagocytizes red blood cells and produces an amebostome to feed off of brain tissue. Along with the ameobostome, N. fowleri secretes hydrolases and phospholases to begin digestion of the brain tissue before phagocytizing it. This highly active phagocytotic activity causes severe hemorraging (C).
Clinical symptoms of PAM mimic that of any other menitigis including headache, fever, nausea and vomiting (C). As stated before, infection of N. fowleri is toxic over 90% of the time. Even f medical care is sought early enough, within a day or two of exposure and infection, the chance of correct diagnosis of PAM as caused by N. fowleri is rare. The most rapid and accurate way to diagnose an N. fowleri is to examine a fresh cerebrospinal tap sample under a light microscope. For future investigation,cultures can be grown and brain tissue can be stained with hematoxylin and eosin, however this culturing and staining process is too lengthy to be used in regards to patient care. The only effective drug against the amoeba is amphotericin B, though other sulfa drugs have been used in collaboration with amphotericin B for a slightly better response.
Current Research
Enter summarries of the most rescent research here--at least three required
