Talk:MicrobeWiki

I am just curious why the page is aligned left?? i think it looks better centered but it's all good!!

Drew T. 6/08/06

Ralstonia paucula

Ralstonia paucula is a Gram-negative environmental bacterium.

Ralstonia paucula was assigned to the genus Ralstonia in 1999. Isolated from pool water, groundwater, bottled mineral water (and many clinical specimens, R. paucula, despite its low pathogenicity, is now recognized as an opportunist pathogen that can generate serious infections, such as septicemia, peritonitis, abscesses, etc., especially in immunocompromised patients Furthermore, though less often isolated than Ralstonia pickettii, R. paucula can cause clusters of nosocomial infections. Treatment of such infections was mainly based on the use of beta-lactams such as cefotaxime, ceftriaxone, piperacillin, and imipenem.[1]

Confronted in 1995 with five cases of nosocomial bacteremia, we found that pulsed-field gel electrophoresis could not distinguish between the isolates and that randomly amplified polymorphic DNA analysis was poorly discriminatory. In this study, we used PCR-ribotyping and PCR-restriction fragment length polymorphism analysis of the spacer 16S-23S ribosomal DNA (rDNA); both methods were unable to differentiate R. paucula isolates.[1]

REFERENCES:

1. Didier Moissenet,Philippe Bidet, Antoine Garbarg-Chenon, Guillaume Arlet, and Hoang Vu-Thien,"Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)",  Journal of Clinical Microbiology, January 2001, p. 381-384, Vol. 39, No. 1