User:Kwala

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Contents [hide] 1 Classification 1.1 Higher order taxa 1.2 Species 2 Description and significance 3 16S Ribosomal RNA Gene Information 4 Cell structure and metabolism 5 Ecology 6 Pathology 7 Current Research 8 References


[edit] Classification [edit] Higher order taxa 

Proteobacteria, Bacteria, Ralstoniaceae, Ralstonia,

RALSTONIA PAUCULA


Genus species

[edit] Description and significance

Ralstonia paucula is a Gram-negative environmental bacterium. Isolated from pool water, groundwater, bottled mineral water, and many clinical specimens. Ralstonia paucula, despite its low pathogenicity, is now recognized as an opportunist pathogen that can generate serious infections, such as septicemia, peritonitis, abscesses, etc., especially in immunocompromised patients

[edit] 16S Ribosomal RNA Gene Information

In 1995, bacteria was confronted with five cases of nosocomial bacteremia, we found that pulsed-field gel electrophoresis could not distinguish between the isolates and that randomly amplified polymorphic DNA analysis was poorly discriminatory. In this study, we used PCR-ribotyping and PCR-restriction fragment length polymorphism analysis of the spacer 16S-23S ribosomal DNA (rDNA); both methods were unable to differentiate R. paucula isolates. Eighteen strains belonging to other Ralstonia species (one R. eutropha strain, six R. pickettii strains, three R. solanacearum strains, and eight R. gilardii strains) were also tested by PCR-ribotyping, which failed to distinguish between the four species. The 16S-23S rDNA intergenic spacer of R. paucula contains the tRNAIle and tRNAAla genes, which are identical to genes described for R. pickettii and R. solanacearum. [1]

[edit] Cell structure and metabolism



[edit] Ecology


[edit] Pathology R. paucula can cause clusters of nosocomial infections. Treatment of such infections was mainly based on the use of beta-lactams such as cefotaxime, ceftriaxone, piperacillin, and imipenem.[1]

[edit] Current Research


[edit] References

1. Didier Moissenet, Philippe Bidet, Antoine Garbarg-Chenon, Guillaume Arlet, and Hoang Vu-Thien1 "Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)", Journal of Clinical Microbiology, January 2001, p. 381-384, Vol. 39, No. 1

Edited by Karolina Wala, Alma Shebib, students of M Glogowskiat Loyola University http://www.ncbi.nlm.nih.gov/pmc/articles/PMC87738/

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