Bdellovibrio

A Microbial Biorealm page on the genus Bdellovibrio

Classification

Higher order taxa

Bacteria; Proteobacteria; Deltaproteobacteria; Bdellobvionales; Bdellovibrionaceae

Species

B. bacteriovorus; B. starrii; B. stolpii

NCBI: Taxonomy

Description and significance

B. bacteriovorus is primarily found in ocean water, freshwater, sewage, soil, and the intestines of certain mammals. It preys on other bacteria, plants, animal, and human pathogens. The bacterium is also known as a “living antibiotic” since it is not the cause of any known diseases.

Genome structure

B. bacteriovorus has a large genome that consists of over three million base pairs on a single circular chromosome. It encodes 3,584 predicted genes and has a single sheathed polar flagellum, which allows the bacterium to move at high speeds (approximately 100 cell lengths per second). B. bacteriovorus has not yet showed the ability to transfer genes from its prey, however it is known to invade and entirely consume other gram-negative bacteria. In addition, B. Bacteriovorus obtains essential nutrients for growth from its prey; it lacks genes that aid in the synthesis and degradation of, certain amino acids. Recent studies have found that its genome consists of a large number of lytic enzymes numbering over 200 genes. These genes assist in the decomposition of the proteins, polysaccharides, RNA, DNA, and lipids that make up its prey's inner cell. Additionally, the steps of its life cycle (including entry into the host) are made possible due to the existence of hydrolytic enzymes in its genome.

Cell structure and metabolism

B. bacteriovorus is a gram-negative rod shaped bacterium that is highly motile. On average, B. bacteriovorus is 0.2 to 0.5 μm by 0.5 to 2.5 μm in size. It thrives on the degradation products of the host’s proteins and nucleic acids. The bacterium is comprised of a helical filament of flagellum that is contained within a membrane sheath; this membrane sheath is then surrounded by a basal sheath. How this bacterium penetrates the outer membrane of its prey is unknown.

The life cycle of B. bacteriovorus includes five stages. First, the bacterium attaches itself to the host's outer membrane using a hook-like extension and breaks down the outer cell wall through the release of certain enzymes. This is followed by penetration into the periplasm and the repair of damage done to the cell membrane. Once the bacterium is inside the periplasm, it introduces hydrolytic enzymes into the host cytoplasm. This increases the bacterium’s food supply by making the inner membrane of the host cell “leaky.” The bacterium then grows without itself dividing and once it has exhausted all the resources in the host’s cell, the bacterium divides into as many as 15 motile cells. The weakened host cell lyses, releasing progeny B. bacteriovirus bacteria, which are then ready to find new prey to attack. This life cycle takes up to three to four hours.

Ecology

This bacterium is commonly found in water of different qualities including some water treatment plants. It can also be found in natural man-made habitats, the rhizosphere of plant roots, and soil. It has also been observed to reside in the intestinal tract of certain mammals where it reduces pathogens in the intestines. B. bacteriovorus is useful in the purification of waste water because it decreases gram-negative bacterial counts.

References

Bacteria Genomes BDELLOVIBRIO BACTERIOVORUS. (2006, August 30). 2Can Support Portal: Genomes- All Genomes. Retrieved November 3, 2006, from http://www.ebi.ac.uk/2can/genomes/genomes.html?http://www.ebi.ac.uk/2can/genomes/bacteria/Bdellovibrio_bacteriovorus.html

Bacterial Nomenclature Up-to-Date. (2004). DSMZ- the German Resource Centre for Biological Material. Retrieved November 3, 2006, from http://www.dsmz.de/microorganisms/bacterial_nomenclature_info.php?genus=Bdellovibrio&show_all_details=1

Bdellovibrio bacteriovorus. (2006). MEROPS- the peptidase database. Retrieved November 3, 2006, from MEROPS database: http://merops.sanger.ac.uk/cgi-bin/speccards?sp=sp002161&type=P

Bdellovibrio genome sequenced. (2006). Genome Biology Research News. Retrieved November 3, 2006, from http://genomebiology.com/researchnews/ default.asp?arx_id=gb-spotlight-20040130-01

Lambert, C., Smith, M. C. M., & Sockett, R. E. (2003, February). A novel assay to monitor predator-prety interaction for Bdellovibrio bacteriovorus 109 J reveals a role for methylaccepting chemotaxis proteins in predation. Environmental Biology, 5(2), 127. Abstract retrieved November 3, 2006, from http://www.blackwell-synergy.com/links/doi/10.1046/ j.1462-2920.2003.00385.x

Shemesh, Y., Davidov, Y., Koval, S., & Edouard, J. (2003). [Review of the scientific experiment Small eats big: ecology and diversity of Bdellovibrio and like organisms, and their dynamics in predator-prey interactions]. EDP Sciences, 23, 433-439. Retrieved November 3, 2006, from http://www.edpsciences.org/articles/agro/pdf/2003/05/A3505.pdf?access=ok

Schwudke, D., Linscheid, M., Strauch, E., Appel, B., Zahringer, U., Moll, H., et al. (2003, July 25). The Obligate Predatory Bdellovibrio bacteriovorus Possesses a Neurtal Lid A Containing {-D-Mannoses That Replace Phosphate Residues. Biol. Chem, 278(30), 27502-27512. Retrieved November 10, 2006, from jbc ONLINE database: http://www.jbc.org/cgi/content/full/278/30/27502

Edited by Swetha Desai, Krystina Eikeseth, Janice Silva, and Derron Simmonds- students of Dr. Kirk Bartholomew