Cenarchaeum
A Microbial Biorealm page on the genus Cenarchaeum
Classification
Domain: Archaea; Phylum: Thaumarchaeota; Order: Cenarchaeales; Family: Cenarchaeaceae; Genus: Cenarchaeum
An important species is Cenarchaeum symbiosum.
Description and significance
Describe the disease caused by this organism if it is a pathogen, or the natural macroscopic "field guide" appearance and habitat of your organism if it is not. What is or has been the impact your organism on human history or our environment?. How does it do this? How have we harnessed this power, or tried to prevent it? In other words, how do you know it if you see it, and how does its presence influence humans in the present, and historically?
Genome structure
Cenarchaeum symbiosum has a single circular chromosome 2,045,086 bp in length. On average, the genome has 57.74% G/C content. The origin(s) of replication are not known. 2,017 protein-encoding genes were predicted and for 65% of these, homology searches showed functional or conserved roles. 47 RNA genes were found in the genome, including 1 operon for a linked small subunit-large subunit ribosomal rRNA, 1 copy of a 5S rRNA, and 45 genes predicted to encode transfer RNAs.
This genome contains most of the core genes generally expected of Archaea. All genes necessary for functional chromosomal replication are also present. Two distinct DNA polymerases are coded for in the genome, one related to those in the thermophilic Crenarchaeota and one similar to euryarchaeotal DNA polymerase II. A eukaryal-like H3-H4 histone was also found in the genome of C. symbiosum, the first crenarchaeote in which this was found.
The genome of C. symbiosum is postulated to contain introns (in 10 of the 45 tRNA genes).
Cell structure, metabolism & life cycle
Cenarchaeum symbiosumexists as rod-shaped cells averaging 0.8 microns long and 0.5 microns wide.
In terms of metabolism, genetic analysis suggests that C. symbiosum uses at least an incomplete TCA cycle. The genome also indicates an intact Embden-Meyerhof-Parnas pathway, although without glucokinase or pyruvate kinase, which suggests that it may form glucose through this pathway rather than breaking it down. However, genes were also found to encode carbohydrate kinases of unknown specificity. Many Entner-Douodoroff pathway components were missing from the genome, indicating that this archaeum does not use the ED pathway for catabolism. However, a nonoxidative pentose phosphate pathway was identified, which could provide NADPH and ribose sugars for anabolism. Homologous genes that could be associated with ammonia oxidation occur in the genome of C. symbiosum, but between missing homologues and identified potential cytochrome replacements for mobile electron carrying suggest that energy derived from ammonia oxidiation is obtained through different mechanisms than those of most nitrifying bacteria.
Ecology (including pathogenesis)
Cenarchaeum symbiosum is psychrophilic, with a natural habitat ~8-18 degrees Celcius. This archaeon forms a symbiosis with an Axinella species (potentially Axinella mexicana, but there were small differences). It was found associated with these sponges at depths of 10-20 meters in the ocean just offshore of Santa Barbara, California. This species is abundant and actively divides when associated with the sponge, and remains associated with the sponge over long periods, which suggests that this is truly a symbiotic relationship.
Interesting feature
Describe in detail one particularly interesting aspect of your organism or it's affect on humans or the environment.
