BT Bacillus Thuringiensis

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Classification

Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]

Species

Eubacteria (kingdom)

Bacteria (domain)

Firmicutes (phylum)

Bacilli (class)

Bacillales (order)

Bacillaceae (family)

Bacillus (genus); Bacillus cereus group

NCBI: Taxonomy

Genus species

Bacillus Thuringiensis

Habitat Information

Describe the location and conditions under which the organism was isolated.

Latitude: 30.14402 degrees Longitude: -97.78506 degrees

It was a cloudy day high of 58 degrees low of 44 degrees on Jan 30th 2015. A spade and a glass mason jar to collet the soil sample. The soil was collected after digging 1 foot below the surface. The location of the soil sample chosen was in a natural ravine off the end of a gated home property Rainfall was 0.0" and the pressure was 28.53" The location was mostly Lewisville silty clay at 1-2 percent slopes.

Description and Significance

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.

Cellular: gram-positive, forms spores, rod-shape

Colonial morphology: filamentous, raised, undulate, medium, aggressive grower, shiny, smooth, creamy white in color

This organism was placed in a lawn plate of Pseudomonas Aeruginosa and it had a zone of inhibition

Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.

BT is a circular genome.

Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.

Physiology and Pathogenesis

Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

References

1.

Author

Page authored by _____, student of Prof. Kristine Hollingsworth at Austin Community College.