Bacillus Pumilus RTMG: Difference between revisions

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(Created page with "{{Uncurated}} ==Classification== Domain; Phylum; Class; Order; family [Others may be used. Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find] ===Species=== {|...")
 
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==Classification==
==Classification==


Domain; Phylum; Class; Order; family [Others may be used.  Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]
<b>Domain:</b> Bacteria


<b>Phylum:</b> Firmicutes
<b>Class:</b> Bacilli
<b>Order:</b> Bacillales
<b>Family:</b> Bacillaceae
<b>Genus:</b> Bacillus
<b>Other Names:</b>
*DSM 28040
*LMG 27940
*Pseudomonas granadensis Pascual et al. 2015
*Pseudomonas sp. F-278,770
*strain F-278,770
===Species===
===Species===


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''Genus species''
<i>Genus species:</i> Pseudomonas granadensis


==Habitat Information ==
==Habitat Information ==
Describe the location and conditions under which the organism was isolated.
Latitude: 30.26 degrees Longitude: 97.69 degrees
 
It was a clear day with a temperature of 64 degrees in the area of Govalle, East Austin on January 25th 2018. A ziplock bag was used to collect the soil from the area mostly from the surface about one inch deep. The location of the soil sample chosen was in a neighborhood field often frequented by dogs. Rainfall was 0.0" and the pressure was 35.35". The description of the location was mostly Bergstrom soils and urban land. 0-2% slopes and rarely flooded.


==Description and Significance==
==Description and Significance==
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.
Cellular: gram negative aerobic
Colonial:


==Genome Structure==
==Genome Structure==
Describe the size and content of the genome.  How many chromosomes?  Circular or linear?  Other interesting features?  What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.
Describe the size and content of the genome.  How many chromosomes?  Circular or linear?  Other interesting features?  What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.


ED2-Reverse_B05.ab1
ACCGTCCTCCCGAAGGTTAGACTAGCTACTTCTGGTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGA
ACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCGATCCGGACTACG
ATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTTTGTACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTA
AGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTG
GTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTG TCTCAATGTTCCCGAAGGCACCAATCCATCTCTGGAAAGTTCATTGGATGTCAAGGCCTGGTAAGGTTCTTCGCGTTGCTTCGAA
TTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCA
ACTTAATGCGTTAGCTGCGCCACTAAGAGCTCAAGGCTCCCAACGGCTAGTTGACATCGTTTACGGCGTGGACTACCAGGGTAT
CTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGTGGTCGCCTTCGCCACTGGTGTTCCTTCCTA
TATCTACGCATTTCACCGCTACACAGGAAATTCCACCACCCTCTACCATACTCTAGCTCGCCAGTTTTGGATGCAGTTCCCAGGT
TGAGCCCGGGGATTTCACATCCAACTTAACGAACCACCTACGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC
TGTATTACCGCG


==Cell Structure, Metabolism and Life Cycle==
==Cell Structure, Metabolism and Life Cycle==
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==Physiology and Pathogenesis==
==Physiology and Pathogenesis==
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br>
<b>BIOCHEMICAL TEST RESULTS</b>
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br>
*<b>Phenol Red Broth Tests:</b> <i>Glucose:</i> positive; <i>Lactose:</i> negative; <i>Sucrose:</i> negative
*<b>Starch Hydrolysis Test:</b> negative
*<b>Casein Hydrolysis Test:</b> negative
*<b>Gelatin Hydrolysis Test:</b> positive
*<b>DNA Hydrolysis Test:</b> slight positive
*<b>Lipid Hydrolysis Test:</b> positive
*<b>Methyl Red Test:</b> negative
*<b>Voges Proskauer Test:</b> negative
*<b>Citrate Test:</b> positive
*<b>SIM Tests:</b> negative for all
*<b>Nitrate Reduction:</b> negative
*<b>Urea Hydrolysis:</b> negative
*<b>Triple Sugar Iron Agar:</b> negative for all
*<b>Oxidase Test:</b> negative
*<b>Eosin Methylene Blue Agar (EMB) Test:</b> positive
*<b>Hektoen Enteric Agar (HE) Test:</b> negative
*<b>MacConkey Agar Test:</b> negative
*<b>Decarboxylation Tests:</b> <i>Arginine:</i> no change; <i>Lysine:</i> negative; <i>Orinithine:</i> negative
*<b> Phenylalanine Deaminase Test:</b> negative
*<b> Catalase Test:</b> negative
*<b> Blood Agar Test:</b> slight positive
*<b>Mannitol Salt Agar (MSA) Test:</b> negative
*<b>Phenylethyl Alcohol Agar (PEA) Test:</b> negative
*<b>Bacitracin/Optochin Susceptibility Test:</b> <i>Bacitracin:</i> resistant; <i>Optochin:</i> resistant
*<b>Bile Esculin Test: </b> negative
*<b>6.5% Salt Tolerance Test: </b>slight positive
 


==References==
==References==
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]
[Sample reference] [http://ijs.sgmjournals.org/cgi/reprint/50/2/489 Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "''Palaeococcus ferrophilus'' gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". ''International Journal of Systematic and Evolutionary Microbiology''. 2000. Volume 50. p. 489-500.]
https://www.ncbi.nlm.nih.gov/pubmed/25410940, "Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain". PubMed.gov. Epub 2014 Nov 19.


==Author==
==Author==
Page authored by _____, student of Prof. Kristine Hollingsworth at Austin Community College.
Page authored by Diane Marques and Emma Riegle, students of Prof. Kristine Hollingsworth at Austin Community College.


<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]]

Revision as of 17:48, 18 April 2018

This student page has not been curated.

Classification

Domain: Bacteria

Phylum: Firmicutes

Class: Bacilli

Order: Bacillales

Family: Bacillaceae

Genus: Bacillus

Other Names:

  • DSM 28040
  • LMG 27940
  • Pseudomonas granadensis Pascual et al. 2015
  • Pseudomonas sp. F-278,770
  • strain F-278,770

Species

NCBI: Taxonomy

Genus species: Pseudomonas granadensis

Habitat Information

Latitude: 30.26 degrees Longitude: 97.69 degrees

It was a clear day with a temperature of 64 degrees in the area of Govalle, East Austin on January 25th 2018. A ziplock bag was used to collect the soil from the area mostly from the surface about one inch deep. The location of the soil sample chosen was in a neighborhood field often frequented by dogs. Rainfall was 0.0" and the pressure was 35.35". The description of the location was mostly Bergstrom soils and urban land. 0-2% slopes and rarely flooded.

Description and Significance

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.


Cellular: gram negative aerobic

Colonial:

Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.

ED2-Reverse_B05.ab1 ACCGTCCTCCCGAAGGTTAGACTAGCTACTTCTGGTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGA ACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCGATCCGGACTACG ATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTTTGTACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTA AGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTG GTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTG TCTCAATGTTCCCGAAGGCACCAATCCATCTCTGGAAAGTTCATTGGATGTCAAGGCCTGGTAAGGTTCTTCGCGTTGCTTCGAA TTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCA ACTTAATGCGTTAGCTGCGCCACTAAGAGCTCAAGGCTCCCAACGGCTAGTTGACATCGTTTACGGCGTGGACTACCAGGGTAT CTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGTGGTCGCCTTCGCCACTGGTGTTCCTTCCTA TATCTACGCATTTCACCGCTACACAGGAAATTCCACCACCCTCTACCATACTCTAGCTCGCCAGTTTTGGATGCAGTTCCCAGGT TGAGCCCGGGGATTTCACATCCAACTTAACGAACCACCTACGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC TGTATTACCGCG

Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.


Physiology and Pathogenesis

BIOCHEMICAL TEST RESULTS

  • Phenol Red Broth Tests: Glucose: positive; Lactose: negative; Sucrose: negative
  • Starch Hydrolysis Test: negative
  • Casein Hydrolysis Test: negative
  • Gelatin Hydrolysis Test: positive
  • DNA Hydrolysis Test: slight positive
  • Lipid Hydrolysis Test: positive
  • Methyl Red Test: negative
  • Voges Proskauer Test: negative
  • Citrate Test: positive
  • SIM Tests: negative for all
  • Nitrate Reduction: negative
  • Urea Hydrolysis: negative
  • Triple Sugar Iron Agar: negative for all
  • Oxidase Test: negative
  • Eosin Methylene Blue Agar (EMB) Test: positive
  • Hektoen Enteric Agar (HE) Test: negative
  • MacConkey Agar Test: negative
  • Decarboxylation Tests: Arginine: no change; Lysine: negative; Orinithine: negative
  • Phenylalanine Deaminase Test: negative
  • Catalase Test: negative
  • Blood Agar Test: slight positive
  • Mannitol Salt Agar (MSA) Test: negative
  • Phenylethyl Alcohol Agar (PEA) Test: negative
  • Bacitracin/Optochin Susceptibility Test: Bacitracin: resistant; Optochin: resistant
  • Bile Esculin Test: negative
  • 6.5% Salt Tolerance Test: slight positive


References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

https://www.ncbi.nlm.nih.gov/pubmed/25410940, "Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain". PubMed.gov. Epub 2014 Nov 19.

Author

Page authored by Diane Marques and Emma Riegle, students of Prof. Kristine Hollingsworth at Austin Community College.

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