Bacillus stearothermophilus NEUF2011: Difference between revisions

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==Description and Significance:==
==Description and Significance:==


Bacillus stearothermophilus is a rod-shaped, Gram-positive, spore forming bacterium. This thermophile grows in temperatures ranging from 30-75 degrees C, and is able to survive temperatures as high as 130 degrees C.  This bacteria can be found in a variety of warm environments and is often the cause of food spoilage. Clinically, it is very a beneficial organism that can be used to verify that laboratory sterilization processes are working properly. 1
Bacillus stearothermophilus is a rod-shaped, Gram-positive, spore forming bacterium. This thermophile grows in temperatures ranging from 30-75 degrees C, and is able to survive temperatures as high as 130 degrees C.  This bacteria can be found in a variety of warm environments and is often the cause of food spoilage. Clinically, it is very a beneficial organism that can be used to verify that laboratory sterilization processes are working properly. [1]


==Genome Structure:==
==Genome Structure:==


Bacillus stearothermophilus has sixty-four genes that were successfully mapped in 1990 by Vallier and Welker of Northwestern University (2). Their findings indicate that the genetic makeup of the Bacillus stearothermophilus and the genetic makeup of a closely related species, Bacillus subtilis, are very similar, with some important differences (2).
Bacillus stearothermophilus has sixty-four genes that were successfully mapped in 1990 by Vallier and Welker of Northwestern University [2]. Their findings indicate that the genetic makeup of the Bacillus stearothermophilus and the genetic makeup of a closely related species, Bacillus subtilis, are very similar, with some important differences [2].


==Cell Structure and Metabolism:==
==Cell Structure and Metabolism:==


Since Bacillus stearothermophilus is a Gram-positive bacteria, it has both an inner and outer membrane (3).  The cell surface of this microbe contains a capsule, a S-layer, several layers of peptidoglycan, and proteins on the outer membrane.  The genus Bacillus have S-layers, which are crystalline layers of surface proteins and like the S-layers of other bacteria, the role of these S-layers is unknown but thought to be connected to the adherence of the bacterial cell (3).  The capsules of Bacillus stearothermophilus are easily observed using light microscopy especially if this bacteria is prepared on growth media that allows for heavy capsule production (3).  The cell wall contains a peptidoglycan that contains DAP or meso-diaminopimelic acid and this bacterial species has a substantial amount of teichoic acids bonded to muramic acid residues (3).   
Since Bacillus stearothermophilus is a Gram-positive bacteria, it has both an inner and outer membrane [3].  The cell surface of this microbe contains a capsule, a S-layer, several layers of peptidoglycan, and proteins on the outer membrane.  The genus Bacillus have S-layers, which are crystalline layers of surface proteins and like the S-layers of other bacteria, the role of these S-layers is unknown but thought to be connected to the adherence of the bacterial cell [3].  The capsules of Bacillus stearothermophilus are easily observed using light microscopy especially if this bacteria is prepared on growth media that allows for heavy capsule production [3].  The cell wall contains a peptidoglycan that contains DAP or meso-diaminopimelic acid and this bacterial species has a substantial amount of teichoic acids bonded to muramic acid residues [3].   


==Ecology:==
==Ecology:==
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==References:==
==References:==


1:NAZINA (T.N.), TOUROVA (T.P.), POLTARAUS (A.B.), NOVIKOVA (E.V.), GRIGORYAN (A.A.), IVANOVA (A.E.), LYSENKO (A.M.), PETRUNYAKA (V.V.), OSIPOV (G.A.), BELYAEV (S.S.) and IVANOV (M.V.): Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans. Int. J. Syst. Evol. Microbiol. 2001, 51, 433-446.
[1] NAZINA (T.N.), TOUROVA (T.P.), POLTARAUS (A.B.), NOVIKOVA (E.V.), GRIGORYAN (A.A.), IVANOVA (A.E.), LYSENKO (A.M.), PETRUNYAKA (V.V.), OSIPOV (G.A.), BELYAEV (S.S.) and IVANOV (M.V.): Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans. Int. J. Syst. Evol. Microbiol. 2001, 51, 433-446.


2:Vallier, H. & Welker, N. E. (1990). Genetic map of the Bacillus stearothermophilus NUB36 chromosome. J Bacteriol 172, 793–801.
[2] Vallier, H. & Welker, N. E. (1990). Genetic map of the Bacillus stearothermophilus NUB36 chromosome. J Bacteriol 172, 793–801.


3:Todar, Kenneth. "Surface Structure of Bacillus." Textbook of Bacteriology. Madison, Wisconsin: , 2008. Web. 5 10, 2011.
[3] Todar, Kenneth. "Surface Structure of Bacillus." Textbook of Bacteriology. Madison, Wisconsin: , 2008. Web. 5 10, 2011.

Revision as of 23:10, 18 October 2011

Bacillus stearothermophilus

Classification:

Higher Order taxa:

Kingdom: Bacteria
Phylum: Firmicutes
Class: Bacilli
Order: Bacillales
Family: Bacillaceae

Species:

Geobacillus stearothermophilus.

Description and Significance:

Bacillus stearothermophilus is a rod-shaped, Gram-positive, spore forming bacterium. This thermophile grows in temperatures ranging from 30-75 degrees C, and is able to survive temperatures as high as 130 degrees C. This bacteria can be found in a variety of warm environments and is often the cause of food spoilage. Clinically, it is very a beneficial organism that can be used to verify that laboratory sterilization processes are working properly. [1]

Genome Structure:

Bacillus stearothermophilus has sixty-four genes that were successfully mapped in 1990 by Vallier and Welker of Northwestern University [2]. Their findings indicate that the genetic makeup of the Bacillus stearothermophilus and the genetic makeup of a closely related species, Bacillus subtilis, are very similar, with some important differences [2].

Cell Structure and Metabolism:

Since Bacillus stearothermophilus is a Gram-positive bacteria, it has both an inner and outer membrane [3]. The cell surface of this microbe contains a capsule, a S-layer, several layers of peptidoglycan, and proteins on the outer membrane. The genus Bacillus have S-layers, which are crystalline layers of surface proteins and like the S-layers of other bacteria, the role of these S-layers is unknown but thought to be connected to the adherence of the bacterial cell [3]. The capsules of Bacillus stearothermophilus are easily observed using light microscopy especially if this bacteria is prepared on growth media that allows for heavy capsule production [3]. The cell wall contains a peptidoglycan that contains DAP or meso-diaminopimelic acid and this bacterial species has a substantial amount of teichoic acids bonded to muramic acid residues [3].

Ecology:

This thermophile and is widely distributed in soil, hot springs and ocean sediment.

Pathology:

Is it pathogenic, which host, what symptoms? Current Research Summarize at least two research articles

Cool factor:

Find something cool about your organism

References:

[1] NAZINA (T.N.), TOUROVA (T.P.), POLTARAUS (A.B.), NOVIKOVA (E.V.), GRIGORYAN (A.A.), IVANOVA (A.E.), LYSENKO (A.M.), PETRUNYAKA (V.V.), OSIPOV (G.A.), BELYAEV (S.S.) and IVANOV (M.V.): Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans. Int. J. Syst. Evol. Microbiol. 2001, 51, 433-446.

[2] Vallier, H. & Welker, N. E. (1990). Genetic map of the Bacillus stearothermophilus NUB36 chromosome. J Bacteriol 172, 793–801.

[3] Todar, Kenneth. "Surface Structure of Bacillus." Textbook of Bacteriology. Madison, Wisconsin: , 2008. Web. 5 10, 2011.