Difference between revisions of "Bartonella quintana"

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{{Uncurated}}
 
{{Biorealm Genus}}
 
{{Biorealm Genus}}
  
 
==Classification==
 
==Classification==
Gram-negative oral spirochete
+
Gram-negative rod shape
  
 
===Higher order taxa===
 
===Higher order taxa===
  
Domain:Spirochaetes,
+
Domain:Bacteria,
Phylum: Bacteria,
+
Phylum: Proteobacteria,
Class: Inside series of Bacteria,
+
Class: Alpha Proteobacteria,
Order:,
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Order: Rhizobiales,
Family: Treponema
+
Family: Bartonellacaea
  
 
Bacteria; Spirochaetes; Spirochaetales; Spirochaetaceae; Treponema.
 
 
Strains:
 
Treponema denticola strain ATCC 35405.
 
 
(there may be additional subcategories included as well.  You can just copy this information from the NCBI taxonomy page)
 
 
Edited by Neena Patel, student of Rachel Larsen at UCSD.
 
  
 
===Genus===  
 
===Genus===  
  
  
Genus species: ''Treponema denticola''
+
Genus species: ''Bartonella Quintana''
  
  
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Edited by Neena Patel, student of Rachel Larsen at UCSD.
 
  
 
==Description and significance==
 
==Description and significance==
Describe the appearance, habitat, etc. of the organism, and why it is important enough to have its genome sequenced.  Describe how and where it was isolated.
 
Include a picture or two (with sources) if you can find them.
 
  
''Treponema denticola'' is a motile, slender, helically shaped flexible organism. It is made up of a periplasmic flagella wound around the helical protoplasmic cylinder and encased in an outer sheath. It's habitat is anaerobic and host-associated. It grows at an optimal temperature of 30-42°C, with a pH of 6.5-8.0. It is commonly found in the human oral cavity, specifically in subgingival dental plaque, and it is often associated with periodontal disease. Periodontal disease results in inflammation of the gum tissue, bone resorbtion, and subsequent tooth loss. Periodontal disease has now become a major concern in dentistry and 80% of adults in the USA are estimated to have had periodontal disease at some point in their lives.
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''Bartonella quintana'' was formally classified in the Rickettsiaceae family as the obligately intracellular (cannot reproduce outside the host cell) ''Rochalimaea quintana'' (28). The difference between Rickettsia and Rochalimaea was the chemical composition in their genomic structure. Rickettsia species have a low level of guanine and cytosine (28.5 – 33.3 mol%) but Rochalimaea and Bartonella species have a higher level (39.0 – 40.0 mol%). The description of these species has increased by the availability of modern taxonomic methods.  A few techniques include DNA hybridization and 16 rRNA gene sequence comparison (4).  
The complete genome of T. denticola strain 35405 was sequenced by using the random shotgun method described for genomes sequenced by The Institute for Genomic Research and it was designated as the type strain by Chan et al.
 
  
 +
''B. quintana'', as opposed to related species ''B. bacilliformis'' and ''B. clarrideiae'' that possess flagella, uses a twitching movement caused by fimbriae.  ''B.quintana'' is a facultative, Gram-negative bacillus belonging to 2 subgroup of proteobacteria.  The dimensions of the bacterium is 0.3 – 0,5 m wide and 1-1.7 m long (7).  It can be cultured in media containing blood or hemin and in a moist 5-10% carbon dioxide atmosphere at 30C to 37C (5). Because ''B. quintana'' is a slow-growing bacterium, primary isolations are available 12 to 14 days after ''B. quintana'' has been embedded and incubated in blood agar at 37C.  Unfortunately, incubation periods could last as long as 45 days that is necessary for primary isolations (3).
  
Edited by Neena Patel, student of Rachel Larsen at UCSD.
+
Humans are the reservoir of the bacterium but the mode of transmission comes directly from a human body louse, ''Pediculus humanus corporis'' (20).  During asymptomatic period, ''B. quintana'' is located in the erythrocytes and has been detected in erythroblasts at the bone marrow (12).  Also, bacillary angiomatosis infection is caused by ''B. quintana'' having an affinity for endothelial cells, leading to angioproliferative lesions (13).  
  
==Genome structure==
+
Currently, ''B. quintana'' has reemerged as an opportunistic infectious agent primarily in immunocompromised patients, especially individuals suffering with HIV infection.  It also causes a febrile disease among alcoholic individuals and the homeless populations in the cities in United States and Europe called “urban trench fever” (11)''B. quintana'' infections can manifest as bacillary angiomatosis, bacillary peliosis, endocarditis, and chronic bacteremia, which can be life-threatening if these conditions occur concurrently (7).  
Describe the size and content of the genomeHow many chromosomes? Circular or linear?  Other interesting features?  What is known about its sequence?
 
Does it have any plasmids?  Are they important to the organism's lifestyle?
 
  
  
Treponema denticola ATCC 35405 has a complete genome. It is made up of dsDNA and 1 chromosome. It is circular and the length is 2,843,201 nucleotides. The GC content is 37%. It has 2838 genes.
+
==Genome structure==
Replicon Type: chromosome.
+
''B. quintana’s'' genome is composed of a single circular chromosome containing 1,581,384 bp.  In addition, 1,308 genes have been identified but only  26 genes are unique to ''B. quintana''. It has been recently discovered that the genome sequence of ''B. quintana'' is a derivative of the larger 1.9 Mb genome of ''B. henselae''.  ''B. quintana'' does not contain genomic islands coding for filamentous hemgglutinin found in ''B. henselae'' (14). Southern blots indicated that all five pathogenic ''Bartonella spp.'' possess hbpA homlogs, which encode an integral membrane protein localized at the outer membrane and surface exposed. HbpA is the first potential virulence determinant characterized from ''B. quintana''(18). In addition, both ''B. quintana'' and ''B. henselae'' genomes are shortened versions of chromosome 1 from the highly related pathogen ''Brucella melitensis'' (14).
  
Edited by Neena Patel, student of Rachel Larsen at UCSD.
 
  
 
==Cell structure and metabolism==
 
==Cell structure and metabolism==
Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.
+
A number of bacterial pathogens have evolved for accumulating hemin in order to satisfy their iron, proporphyrin ring, or cytcochrome cofactor requirements.  However, early studies with ''B. quintana'' indicated that iron is the critical component provided by hemin supplements (15).  The transport of iron from hemin is a TonB-dependent process that requires the synthesis of a surface-membrane receptor to facilitate the binding of the ligand. Next, a protein in the periplasmic space transports the hemin to the cytoplasmic membrane, and finally a permease brings the molecule into the bacterial cell (16).
  
The genome reveals factors mediating coaggregation, cell signaling, and stress protection. It has a spiral shape and is arranged in singles. It is a mobile organism but does not contain any endospores. Motility is by rapid rotation around the long axis, flexation of the cell and locomotion along a helical path. The most distinctive property is the presence of periplasmic flagella wound around the helical protoplasmic cylinder and encased in an outer sheath.
+
In early 1960’s, Vinson first cultured ''B. quintana'' in axenic media, either in blood–enriched agar or in broth with amino acids, yeast extract, and fetal bovine serum (2). These microorganisms obtain their source of energy from succinate, pyruvate, and glutamate but are unable to use glucose.  In addition, bicarbonate is essential for ''B. quintana'' as a source of carbon dioxide (1).  Because ''Bartonella spp''. are the only bacterial pathogens that engage in hemotrophy (erythrocyte parasitism), all ''Bartonella'' species require erythrocytes or hemin supplements in order to grow in vitro. ''B. quintana'' has the highest hemin requirement (20 to 40 µg/ml of medium) for a bacterium (15). There is a family of hemin binding proteins (HbpA, HbpB, HbpC, HbpD, and HbpE) that are synthesized by ''B. quintana'' and serve as hemin receptors (18).  However, the reason for this unique characterization of ''B. quintana'' hemin requirement and mechanism is still unclear. Although HbpA plays a role in the ability of ''B. quintana'' to acquire hemin from the environment (abiotic or biotic), antibiotics specific for HbpA may inhibit the ligand-receptor interaction (17). Because HbpA is only one of the eight membrane-associated proteins in the organism, hemin binding is not abolished in the presence of anti-HbpA Fab fragments.  The exact localization and involvement in iron acquisition of the additional hemin-binding membrane proteins is currently under investigation (18).
 +
 
 +
Pili and specialized extensions of the outer membrane are thought to play an important role in adhesin to nucleated cells.  Pili structures in ''B. henselae'', ''B. quintana'', ''B. tribocorum'', and ''B. alsatica'' possess type IV pili. Twitching motility and self-aggregation are specific properties typical for this type of pili (19).
  
Edited by Neena Patel, student of Rachel Larsen at UCSD.
 
  
 
==Ecology==
 
==Ecology==
Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.
+
Since ''B. quintana'' is able to produce a variety of diseases, it must be able to adapt, survive and replicate in different environments of the ''Pediculus humanus coproris'' gut and the human immune system.  A recent study by Minnick examined environmental factors (heme, oxgen, and temperature) that regulate transcription, regulation, and synthesis of the virulence factor (Hbps) of ''B. quintana''.  First, hbpC expression is regulated by temperature.  In effect, HbpC synthesis is greatest at cooler body temperature of the body louse.  Second, the cultivation of the bacterium in human bloodstream oxygen concentration decreases the expression of all hpb genes.  Minnick indicated that synthesis of HbpC is influenced by oxygen (5% relative to 21% atmospheric) and/or reactive oxygen species.  Third, different expression patterns of hbp had different effects when grown in a range of hemin concentrations.  A high heme concentration stimulates expression of hbpC and hbpB and a low heme concentration stimulates expression of hbpA, hbpD, hbpE.  It’s been considered that the high hemin requirement is essential for replication to maintain pathogenic property of ''B. quintana'' (21).
  
 
==Pathology==
 
==Pathology==
''Treponema denticola'' is a bacterial pathogen and plant plastid. It causes periodontal disease and gum inflammation.
+
''B. quintana'' is identified as a bacterial agent of trench fever that infected more than one million troops and prisoners living in crowded, unhygienic conditions in the First World War (8). By 1915, the human body louse was recognized as the insect vector of ''B. quintana'' through experimentations on volunteer soldiers (6). People are infected by inoculation of the organism in louse feces through a break in the skin, either from the bite of the louse or other means.  Infected lice begin to excrete infectious feces 5-12 days after ingesting infective blood, which continues throughout the remainder of their life span (29). The first experiment demonstrated volunteer soldiers contained “rickettsia bodies” in their gut when blood was transferred from trench fever patients.  In the second experiment, Kosterzewski confirmed that body lice were the primary vectors of ''B. quintana'' (10). This was observed when macaque monkeys were infected after they were injected with blood from typhus patients.  Therefore, the transmission of the disease between monkeys was by means of body louse, the same body louse that was found on the uniforms of soldiers infected with trench fever and typhus.  Thus, an effective transmission control consisted of washing and delousing procedures (9).
  
 +
Clinical manifestations of trench fever range from asymptomatic infection to severe, life-threatening illness.  Few of the common symptoms include headache, pain in the legs and loins, constipation, insomnia, dyspnea, and abdominal pain.  Trench fever often results in prolonged disability but no fatalities have been recorded.  Patients are profoundly ill in the early state of the disease and continue for 4 to 6 weeks.  A minor illness could become chronic – Byman defined chronic trench fever as “a state of marked debility, with or without attacks of slight fever and aching, and characterized by a hyperexcitability of the nervous system in general” (29).  After the primary infection of trench fever resolves, chronic bacteremia develops.  After 8 years of experiments, Kostrzewski confirmed that ''B. quintana'' was present in the blood of trench fever patients; therefore, persistent bacteriemia is associated with ''B. quintana'' infection (10).  In addition, patients with bacillary angiomatosis and endocartisis have also reported being infected with bacteremia (30).
  
How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
+
Bartonella are unique among bacterial pathogens in their ability to cause angioproliferative lesions, involving proliferation of endothelial cells.  Immunocompromised patients are highly susceptible to bacillary angiomatosis, predominately patients with AIDS (23).  ''B. quintana'' and ''B. henselae'' are the agents of bacillary angiomatosis (31).  Bacillary agniomatosis often involves infection of the skin, but various organs could be affected, including the liver, spleen, bone marrow, and lymph nodes. Cutaneous lesions may be solitary or multiple; they may be superficial, dermal, or subcutaneous. Deep lesions may be red, purple, or uncolored.  Deep lesions are colorless and either mobile or fixed to underlying structures (7).  Subcutaneous lesions may erode into underlying bones are associated with ''B. quintana'' whereas hepatic peliosis and lymph node lesions are associated with ''B. henselae'' (31).  Some of the symptoms include fever, abdominal pain, malaise, and vomiting (peliosis hepatitis).
  
Edited by Neena Patel, student of Rachel Larsen at UCSD.
+
There have been previous reported cases that ''B. quintana'' causes lymphadenoapthy.  A non-HIV infected women was diagnosed with chronic cervical and mediastinal adenoapathy (3).  She had an enlarged lymph node removed and samples of the blood were collected.  Histological examinations of cervical lymph node and bone marrow showed a granulomatous reaction.  In a recent study, an HIV-infected patient with supraclavicular inflammatory lymphadenitis had a coinfection of ''B.quintana'' and ''Mycobacterium tuberculosis'' (32).  
  
==Application to Biotechnology==
 
Does this organism produce any useful compounds or enzymes?  What are they and how are they used?
 
  
 
==Current Research==
 
==Current Research==
  
Enter summaries of the most recent research here--at least three required
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In a recent study, Kemf attempted to understand the interaction of ''B. quintana'' with human macrophages (THP-1) and epithelial cells (HeLa 229).  The goal of the experiment was to analyze the host cell interaction (adhesin, invasion, and VEGF induction) and binding of ''B. quintana'' using macrophages and epithelial cells.  Only JK-31 strain of ''B. quintana'' induced secretion of vascular endothelial growth factor (VEFG) from THP-1 and HeLa 229.  It was observed that ''B. quintana'' strains that did not express variable outer membrane protein (Vomps) were unable to induce VEGF secretion through immunofluoroscence testing and electron microscopy.  However, VEGF secretion by JK-31 had no relation with the host cell adherence rates compared with the rates for Vomp-negative ''B. quintana'' strains.  Their results indicated that Vomp does not play a role in the adherence to the host cells (25). 
 +
 
 +
Previous studies have attributed to ''B. quintana'' the unique ability to induce angioproliferative lesions by direct mitotic stimulation of endothelial cells, a significant pathogenic characteristic that has been studied on cultured human endothelial cells.  An experiment has expanded this concept by showing that Bartonella inhibits apoptosis of endothelial cells in vitro, and its ability to stimulate proliferation of endothelial cells depending on its antiapoptotic activity.  Their hypothesis stated that an increase in cell numbers are caused by either increase in cell division a reduction in cell death.  They concluded that an increase number of endothelial cells were caused predominantly by decreased cell death.  It was suggested that under normal conditions, endothelial cells normally respond to infection by undergoing apoptosis but Bartonella has evolved an antiapoptotic activity, a common defense mechanism to combat the host defense mechanism. It was proposed that Bartonella’s antiapoptotic mechanism plays a role to induce vascular proliferation in vitro by suppressing both early and late events in apoptosis (22).
 +
 
 +
Because examination of dental pulp is similar to examination of blood samples, scientists detected DNA of ''B. quintana'' in the dental pulp from 4000 year old human remains in southeastern France.  This was the first study that demonstrated the presence of ''B. quintana'' DNA in ancient human remains.  ''B. quintana'' hemin binding protein E gene was amplified using PCR and sequencing reactions yielded 2 fragments of ''B. quintana''.  The base positions had 97% similarity with the modern ''B. quintana'' groEL gene.  However, it is not known if the body louse was the vector.  There is little evidence when the ectoparasites first infected humans, but eggs have been found in ancient Israel and Greenland (26).  These are only few observations that cannot analyze the history of the body louse pathogenic evolution.  Therefore, more experiments must be attempted to develop new information with the coevolution of ''B.quintana'' and human relationship (27).
  
 
==References==
 
==References==
example:
 
  
[http://www.pnas.org/cgi/content/full/100/14/8298 Glockner, F. O., M. Kube, M. Bauer, H. Teeling, T. Lombardot, W. Ludwig, D. Gade, A. Beck, K Borzym, K Heitmann, R. Rabus, H. Schlesner, R. Amann, and R. Reinhardt. 2003. "Complete genome sequence of the marine planctomycete ''Pirellula'' sp. strain 1."'' Proceedings of the National Acedemy of Sciences'', vol. 100, no. 14. (8298-8303)]
+
 
 +
1. Weiss, E. 1981. Biochemistry and metabolism of rickettsiae: current trends, p. 387–400. In W. Burgdorfer and R. L. Anacker (ed.), Rickettsiae and rickettsial diseases. Academic Press, Inc., New York.
 +
 
 +
2. Weiss, E., G. A. Dasch, D. R. Woodman, and J. C. Williams. 1978. Vole agent identified as a strain of the trench fever rickettsia, Rochalimaea quintana. Infect. Immun. 19:1013–1020.
 +
 
 +
3. Maurin, M., V. Roux, A. Stein, F. Ferrier, R. Viraben, and D. Raoult. 1994. Isolation and characterization by immunofluorescence, sodium dodecyl sulfate polyacrylamide gel electrophoresis, Western blot, restriction fragment length polymorphism-PCR, 16S rRNA gene sequencing, and pulsed-field gel electrophoresis of Rochalimaea quintana from a patient with bacillary angiomatosis. J. Clin. Microbiol. 32:1166–1171.
 +
 
 +
4. Brenner, D. J., S. P. O’Connor, H. H. Winkler, and A. G. Steigerwalt. 1993. Proposals to unify the genera Bartonella and Rochalimaea with descriptions of Bartonella quintana comb. nov., Bartonella vinsonii comb. nov., Bartonella henselae comb. nov., and Bartonella elizabethae comb. nov., and to remove the family Bartonellaceae from the Order Rickettsiales. Int. J. Syst. Bacteriol. 43:777–786.
 +
 
 +
5. Ames G F, Spudich E N, Nikaido H. Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations. J Bacteriol. 1974;117:406–416.
 +
 
 +
6. Alsmark, C. M., A. C. Frank, E. O. Karlberg, B. A. Legault, D. H. Ardell, B. Canback, A. S. Eriksson, A. K. Naslund, S. A. Handley, M. Huvet, S. B. La, M. Holmberg, and S. G. Andersson. 2004. The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae. Proc. Natl. Acad. Sci. USA 101:9716-9721.
 +
 
 +
7. Maurin M, Raoult D. Bartonella (Rochalimaea) quintana infections. Clin Microbiol Rev 1996; 9:273-292.
 +
 
 +
8. Karem KL, Paddock CD, Regnery RL. Bartonella henselae, B. quintana, and B.bacilliformis: historical pathogens of emerging significance. Microbes Infect. 2000;2:1193–205.
 +
 
 +
9. Foucault C, Ranque S, Badiaga S, RoveryC, Raoult D, Brouqui P. Oral ivermectin in the treatment of body lice. J Infect Dis. 2006; In press.
 +
 
 +
10. Kostrzewski J. The epidemiology of trench fever. Bull Acad Pol Sci (Med). 1949;7:233–63.
 +
 
 +
11. Spach DH, Kanter AS, Dougherty MJ, Larson AM, Coyle MB, Brenner DJ, et al. Bartonella (Rochalimaea) quintana bacteremia in inner-city patients with chronic alcoholism. N Engl J Med. 1995;332:424–8.
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 +
12. Rolain JM, Foucault C, Guieu R, La Scola B, Brouqui P, Raoult D. Bartonella quintana in human erythrocytes. Lancet. 2002;360:226–8.
 +
 
 +
13. Meghari S, Rolain JM, Grau GE, Platt E, Barrassi L, Mege JL, et al. Antiangiogenic effect of erythromycin: an in vitro model of Bartonella quintana infection. J Infect Dis. 2006;193:380–6.
 +
 
 +
14. Alsmark CM, Frank AC, Karlberg EO, Legault BA, Ardell DH,Canback B, et al. The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae. Proc Natl Acad Sci U S A. 2004;101:9716–21.
 +
 
 +
15. Myers W F, Cutler L D, Wisseman C L. Role of erythrocytes and serum in the nutrition of Rickettsia quintana. J Bacteriol. 1969;97:663–666.
 +
 
 +
16. Lee B C. Quelling the red menace: haem capture by bacteria. Mol Microbiol. 1995;18:383–390.
 +
 
 +
17. Gray-Owen S D, Schryvers A B. The interaction of primate transferrins with receptors on bacteria pathogenic to humans. Microb Pathog. 1993;14:389–398.
 +
 
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18. Carroll, J.A, S.A. Coleman, L.S. Smitherman, and M. F. Minnick 2000.  Hemin-binding surface protein from Bartonella quintana.  Infect. Immun. 68:6750-7657.
 +
 
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19. Batterman HJ, Peek JA, Loutit JS, Falkow S, Tompkins LS. 1995. Bartonella henselae and Bartonella quintana adherence to and entry into cultured human epithelial cells. Infect. Immun. 63:4553–56.
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20. Raoult D, Roux V. The body louse as a vector of reemerging human diseases. Clin Infect Dis. 1999;29:888–911.
 +
 
 +
21. James M. Battisti, Kate N. Sappington, Laura S. Smitherman, Nermi L. Parrow, and Michael F. Minnick. 2006.  Environmental Signals Generate a Differential and Coordinated Expression of the Heme Receptor Gene Family of Bartonella quintana.  Infect. Immun. 74(6): 3251–3261.
 +
 
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22. James E. Kirby and Dawn M. Nekorchuk. 2002. Bartonella-associated endothelial proliferation depends on inhibition of apoptosis. Proc. Natl. Acad. Sci. USA. 99:4656–4661.
 +
 
 +
23. Spach D H, Koehler J E. Infect Dis Clin North Am. 1998;12:137–155.
 +
 
 +
24. Dehio C. 2001. Bartonella interactions with endothelial cells and erythrocytes. Microbiol. 9:279–85.
 +
 
 +
25. Berit Schulte, Dirk Linke, Sandra Klumpp, Martin Schaller, Tanja Riess, Ingo B. Autenrieth, and Volkhard A.
 +
J. Kempf. Bartonella quintana Variably Expressed Outer Membrane Proteins Mediate Vascular Endothelial Growth Factor Secretion but Not Host Cell Adherence. 2006. Infect Immun. 74: 5003–5013.  
 +
 
 +
26. Sadler JP. Records of ectoparasites on humans and sheep from Viking-age deposits in the former western settlement of Greenland. J Med Entomol 1990; 27:62831.
 +
 
 +
27. Michel Drancourt, Lam Tran-Hung, Jean Courtin,Henry de Lumley, and Didier Raoult. Bartonella quintana in a 4000-Year-Old Human Tooth. 2005. The Journal of Infectious Diseases. 191:607-611.
 +
 
 +
28. Weiss, E., and J. W. Moulder. 1984. Order I, Rickettsiales, p. 687–688. In N. R. Krieg and J. G. Holt (ed.). Bergey’s manual of systematic bacteriology, vol. 1. The Williams & Wilkins Co., Baltimore.
 +
 
 +
29. Byam, W., J. H. Carroll, J. H. Churchill, L. Dimond, V. E. Sorapure, R. M. Wilson, and L. L. Lloyd (ed.). 1919. Trench fever: a louse-born disease. Oxford University Press, London.
 +
 
 +
30. Maurin, M., V. Roux, A. Stein, F. Ferrier, R. Viraben, and D. Raoult. 1994. Isolation and characterization by immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blot, restriction fragment length polymorphism-PCR, 16S rRNA gene sequencing, and pulsed-field gel electrophoresis of Rochalimaea quintana from a patient with bacillary angiomatosis. J. Clin. Microbiol. 32:1166–1171.
 +
 
 +
31. Koehler JE, Sanchez MA, Garrido CS, Whitfeld MJ, Chen FM, Berger TG, et al. Molecular epidemiology of Bartonella infections in patients with bacillary angiomatosis-peliosis. N Engl J Med.1997;337:1876–83.
 +
 
 +
32. Raoult D, Drancourt M, Carta A, Gastaut JA. Bartonella (Rochalimaea) quintana isolation in patient with chronic adenopathy, lymphopenia, and a cat. Lancet. 1994;343:977.
 +
 
  
  
Edited by student of [mailto:ralarsen@ucsd.edu Rachel Larsen] and Kit Pogliano
+
Edited by Rani Thamawatanakul student of Rachel Larsen [mailto:ralarsen@ucsd.edu Rachel Larsen] and Kit Pogliano

Latest revision as of 19:26, 18 August 2010

This student page has not been curated.

A Microbial Biorealm page on the genus Bartonella quintana

Classification

Gram-negative rod shape

Higher order taxa

Domain:Bacteria, Phylum: Proteobacteria, Class: Alpha Proteobacteria, Order: Rhizobiales, Family: Bartonellacaea


Genus

Genus species: Bartonella Quintana


NCBI: Taxonomy


Description and significance

Bartonella quintana was formally classified in the Rickettsiaceae family as the obligately intracellular (cannot reproduce outside the host cell) Rochalimaea quintana (28). The difference between Rickettsia and Rochalimaea was the chemical composition in their genomic structure. Rickettsia species have a low level of guanine and cytosine (28.5 – 33.3 mol%) but Rochalimaea and Bartonella species have a higher level (39.0 – 40.0 mol%). The description of these species has increased by the availability of modern taxonomic methods. A few techniques include DNA hybridization and 16 rRNA gene sequence comparison (4).

B. quintana, as opposed to related species B. bacilliformis and B. clarrideiae that possess flagella, uses a twitching movement caused by fimbriae. B.quintana is a facultative, Gram-negative bacillus belonging to 2 subgroup of proteobacteria. The dimensions of the bacterium is 0.3 – 0,5 m wide and 1-1.7 m long (7). It can be cultured in media containing blood or hemin and in a moist 5-10% carbon dioxide atmosphere at 30C to 37C (5). Because B. quintana is a slow-growing bacterium, primary isolations are available 12 to 14 days after B. quintana has been embedded and incubated in blood agar at 37C. Unfortunately, incubation periods could last as long as 45 days that is necessary for primary isolations (3).

Humans are the reservoir of the bacterium but the mode of transmission comes directly from a human body louse, Pediculus humanus corporis (20). During asymptomatic period, B. quintana is located in the erythrocytes and has been detected in erythroblasts at the bone marrow (12). Also, bacillary angiomatosis infection is caused by B. quintana having an affinity for endothelial cells, leading to angioproliferative lesions (13).

Currently, B. quintana has reemerged as an opportunistic infectious agent primarily in immunocompromised patients, especially individuals suffering with HIV infection. It also causes a febrile disease among alcoholic individuals and the homeless populations in the cities in United States and Europe called “urban trench fever” (11). B. quintana infections can manifest as bacillary angiomatosis, bacillary peliosis, endocarditis, and chronic bacteremia, which can be life-threatening if these conditions occur concurrently (7).


Genome structure

B. quintana’s genome is composed of a single circular chromosome containing 1,581,384 bp. In addition, 1,308 genes have been identified but only 26 genes are unique to B. quintana. It has been recently discovered that the genome sequence of B. quintana is a derivative of the larger 1.9 Mb genome of B. henselae. B. quintana does not contain genomic islands coding for filamentous hemgglutinin found in B. henselae (14). Southern blots indicated that all five pathogenic Bartonella spp. possess hbpA homlogs, which encode an integral membrane protein localized at the outer membrane and surface exposed. HbpA is the first potential virulence determinant characterized from B. quintana(18). In addition, both B. quintana and B. henselae genomes are shortened versions of chromosome 1 from the highly related pathogen Brucella melitensis (14).


Cell structure and metabolism

A number of bacterial pathogens have evolved for accumulating hemin in order to satisfy their iron, proporphyrin ring, or cytcochrome cofactor requirements. However, early studies with B. quintana indicated that iron is the critical component provided by hemin supplements (15). The transport of iron from hemin is a TonB-dependent process that requires the synthesis of a surface-membrane receptor to facilitate the binding of the ligand. Next, a protein in the periplasmic space transports the hemin to the cytoplasmic membrane, and finally a permease brings the molecule into the bacterial cell (16).

In early 1960’s, Vinson first cultured B. quintana in axenic media, either in blood–enriched agar or in broth with amino acids, yeast extract, and fetal bovine serum (2). These microorganisms obtain their source of energy from succinate, pyruvate, and glutamate but are unable to use glucose. In addition, bicarbonate is essential for B. quintana as a source of carbon dioxide (1). Because Bartonella spp. are the only bacterial pathogens that engage in hemotrophy (erythrocyte parasitism), all Bartonella species require erythrocytes or hemin supplements in order to grow in vitro. B. quintana has the highest hemin requirement (20 to 40 µg/ml of medium) for a bacterium (15). There is a family of hemin binding proteins (HbpA, HbpB, HbpC, HbpD, and HbpE) that are synthesized by B. quintana and serve as hemin receptors (18). However, the reason for this unique characterization of B. quintana hemin requirement and mechanism is still unclear. Although HbpA plays a role in the ability of B. quintana to acquire hemin from the environment (abiotic or biotic), antibiotics specific for HbpA may inhibit the ligand-receptor interaction (17). Because HbpA is only one of the eight membrane-associated proteins in the organism, hemin binding is not abolished in the presence of anti-HbpA Fab fragments. The exact localization and involvement in iron acquisition of the additional hemin-binding membrane proteins is currently under investigation (18).

Pili and specialized extensions of the outer membrane are thought to play an important role in adhesin to nucleated cells. Pili structures in B. henselae, B. quintana, B. tribocorum, and B. alsatica possess type IV pili. Twitching motility and self-aggregation are specific properties typical for this type of pili (19).


Ecology

Since B. quintana is able to produce a variety of diseases, it must be able to adapt, survive and replicate in different environments of the Pediculus humanus coproris gut and the human immune system. A recent study by Minnick examined environmental factors (heme, oxgen, and temperature) that regulate transcription, regulation, and synthesis of the virulence factor (Hbps) of B. quintana. First, hbpC expression is regulated by temperature. In effect, HbpC synthesis is greatest at cooler body temperature of the body louse. Second, the cultivation of the bacterium in human bloodstream oxygen concentration decreases the expression of all hpb genes. Minnick indicated that synthesis of HbpC is influenced by oxygen (5% relative to 21% atmospheric) and/or reactive oxygen species. Third, different expression patterns of hbp had different effects when grown in a range of hemin concentrations. A high heme concentration stimulates expression of hbpC and hbpB and a low heme concentration stimulates expression of hbpA, hbpD, hbpE. It’s been considered that the high hemin requirement is essential for replication to maintain pathogenic property of B. quintana (21).

Pathology

B. quintana is identified as a bacterial agent of trench fever that infected more than one million troops and prisoners living in crowded, unhygienic conditions in the First World War (8). By 1915, the human body louse was recognized as the insect vector of B. quintana through experimentations on volunteer soldiers (6). People are infected by inoculation of the organism in louse feces through a break in the skin, either from the bite of the louse or other means. Infected lice begin to excrete infectious feces 5-12 days after ingesting infective blood, which continues throughout the remainder of their life span (29). The first experiment demonstrated volunteer soldiers contained “rickettsia bodies” in their gut when blood was transferred from trench fever patients. In the second experiment, Kosterzewski confirmed that body lice were the primary vectors of B. quintana (10). This was observed when macaque monkeys were infected after they were injected with blood from typhus patients. Therefore, the transmission of the disease between monkeys was by means of body louse, the same body louse that was found on the uniforms of soldiers infected with trench fever and typhus. Thus, an effective transmission control consisted of washing and delousing procedures (9).

Clinical manifestations of trench fever range from asymptomatic infection to severe, life-threatening illness. Few of the common symptoms include headache, pain in the legs and loins, constipation, insomnia, dyspnea, and abdominal pain. Trench fever often results in prolonged disability but no fatalities have been recorded. Patients are profoundly ill in the early state of the disease and continue for 4 to 6 weeks. A minor illness could become chronic – Byman defined chronic trench fever as “a state of marked debility, with or without attacks of slight fever and aching, and characterized by a hyperexcitability of the nervous system in general” (29). After the primary infection of trench fever resolves, chronic bacteremia develops. After 8 years of experiments, Kostrzewski confirmed that B. quintana was present in the blood of trench fever patients; therefore, persistent bacteriemia is associated with B. quintana infection (10). In addition, patients with bacillary angiomatosis and endocartisis have also reported being infected with bacteremia (30).

Bartonella are unique among bacterial pathogens in their ability to cause angioproliferative lesions, involving proliferation of endothelial cells. Immunocompromised patients are highly susceptible to bacillary angiomatosis, predominately patients with AIDS (23). B. quintana and B. henselae are the agents of bacillary angiomatosis (31). Bacillary agniomatosis often involves infection of the skin, but various organs could be affected, including the liver, spleen, bone marrow, and lymph nodes. Cutaneous lesions may be solitary or multiple; they may be superficial, dermal, or subcutaneous. Deep lesions may be red, purple, or uncolored. Deep lesions are colorless and either mobile or fixed to underlying structures (7). Subcutaneous lesions may erode into underlying bones are associated with B. quintana whereas hepatic peliosis and lymph node lesions are associated with B. henselae (31). Some of the symptoms include fever, abdominal pain, malaise, and vomiting (peliosis hepatitis).

There have been previous reported cases that B. quintana causes lymphadenoapthy. A non-HIV infected women was diagnosed with chronic cervical and mediastinal adenoapathy (3). She had an enlarged lymph node removed and samples of the blood were collected. Histological examinations of cervical lymph node and bone marrow showed a granulomatous reaction. In a recent study, an HIV-infected patient with supraclavicular inflammatory lymphadenitis had a coinfection of B.quintana and Mycobacterium tuberculosis (32).


Current Research

In a recent study, Kemf attempted to understand the interaction of B. quintana with human macrophages (THP-1) and epithelial cells (HeLa 229). The goal of the experiment was to analyze the host cell interaction (adhesin, invasion, and VEGF induction) and binding of B. quintana using macrophages and epithelial cells. Only JK-31 strain of B. quintana induced secretion of vascular endothelial growth factor (VEFG) from THP-1 and HeLa 229. It was observed that B. quintana strains that did not express variable outer membrane protein (Vomps) were unable to induce VEGF secretion through immunofluoroscence testing and electron microscopy. However, VEGF secretion by JK-31 had no relation with the host cell adherence rates compared with the rates for Vomp-negative B. quintana strains. Their results indicated that Vomp does not play a role in the adherence to the host cells (25).

Previous studies have attributed to B. quintana the unique ability to induce angioproliferative lesions by direct mitotic stimulation of endothelial cells, a significant pathogenic characteristic that has been studied on cultured human endothelial cells. An experiment has expanded this concept by showing that Bartonella inhibits apoptosis of endothelial cells in vitro, and its ability to stimulate proliferation of endothelial cells depending on its antiapoptotic activity. Their hypothesis stated that an increase in cell numbers are caused by either increase in cell division a reduction in cell death. They concluded that an increase number of endothelial cells were caused predominantly by decreased cell death. It was suggested that under normal conditions, endothelial cells normally respond to infection by undergoing apoptosis but Bartonella has evolved an antiapoptotic activity, a common defense mechanism to combat the host defense mechanism. It was proposed that Bartonella’s antiapoptotic mechanism plays a role to induce vascular proliferation in vitro by suppressing both early and late events in apoptosis (22).

Because examination of dental pulp is similar to examination of blood samples, scientists detected DNA of B. quintana in the dental pulp from 4000 year old human remains in southeastern France. This was the first study that demonstrated the presence of B. quintana DNA in ancient human remains. B. quintana hemin binding protein E gene was amplified using PCR and sequencing reactions yielded 2 fragments of B. quintana. The base positions had 97% similarity with the modern B. quintana groEL gene. However, it is not known if the body louse was the vector. There is little evidence when the ectoparasites first infected humans, but eggs have been found in ancient Israel and Greenland (26). These are only few observations that cannot analyze the history of the body louse pathogenic evolution. Therefore, more experiments must be attempted to develop new information with the coevolution of B.quintana and human relationship (27).

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Edited by Rani Thamawatanakul student of Rachel Larsen Rachel Larsen and Kit Pogliano