Batrachochytrium dendrobatidis

Introduction

Chytrid fungus, Batrachochytrium dendrobatidis http://www.newislander.com/ports/2009/11/say_goodbye_to_kermit/

Fungal disease has emerged as an increasing threat to a number of different organisms, especially amphibian species. Batrachochytrium dendrobatidis, a chytrid fungus that causes the infectious disease chytridiomycosis has been found to be the major cause of amphibian death caused by fungal infection. B. dendrobatidis infects the superficial, keratin-containing layers of amphibian skin. The infection then spreads across the skin, causing it to thicken and slough off. This thickening interferes with osmoregulation and eventually leads to death¹. It has been found to affect at least 93 amphibian species in frogs, toads (Anura) and salamanders (Caudata)². Evidence of B. dendrobatidis has been found worldwide and is believed to have originated in Africa and spread through the trade of Xenopus laevis³. B. dendrobatidis has been responsible for the epidemic illness and death of amphibian populations in Australia, New Zealand, Europe, Central America, and the United States⁴.

Batrachochytrium dendrobatidis

Physiology
Temperature has an effect on mortality rates associated with B. dendrobatidis. B. dendrobatidis can grows in 4-25^oC, but grows optimally in 17-25^oC. Nitrogen has a strong effect on the growth of B. dendrobatidis. It is possible that B. dendrobatidis grow in keratinized epidermal cells because they are dead and easier to invade ⁵.

Morphology
B. dendrobatidis is identified by its intracellular flask-shaped sporangia (spore containing bodies) and septate thalli. The fungus produces smooth-walled zoosporangia (zoospore containing bodies), which are generally spherical in shape. Each zoosporangium (5.2 ± 0.72 μm⁶) produces a single discharge tube, which penetrates the skin and releases mature zoospores. The zoospores (3-5 μm in diameter) are oval shaped and have a single posterior flagellum (19–20 μm in length), which allows it to move in water. There is little morphological difference in B. dendrobatidis strains^4,7

Life cycle of Batrachochytrium dendrobatidis http://theworldofrogs.weebly.com/chytrid-fungus.html

Life Cycle
B. dendrobatidis progresses from a zoospore to a thallus (growing organism), which then produces a single zoosporangium (container for zoospores). The sporangium (contents of the zoosporangium) divides into new zoospores that exit the sporangium through one or more papillae. B. dendrobatidis has not been shown to reproduce sexually. The duration of the life cycle in vitro is 4 to 5 d at 22°C and due to the similarity in life cycles in vivo and in vitro, duration is assumed to be the same in both. B. dendrobatidis infects the superficial epidermis (stratum granulosum and stratum corneum) in amphibians. Immature sporangia are found in deeper cells, while mature zoosporangia and empty sporangia are found in the outer keratinized layers of the skin. Zoospores are released into the environment by discharge tubes that project out of the skin surface⁸.

Pathogen

Toads killed by Batrachochytrium dendrobatidis http://www.zslblogs.org/2011/11/11/a-fungal-%E2%80%98superbug%E2%80%99-causes-global-amphibian-declines/

Chytridiomycosis
B. dendrobatidis infects superficial layers of the skin in amphibians which contain keratin. During metamorphosis skin becomes increasingly keratinized, which allows the infection to spread over the organism. In juvaniles and adults the digits and pelvic patch of skin involved in osmoregulation are the most effected areas. As the infection continues the skin thickens and sloughs off. The skin in amphibians is intimately tied to regulation of hydration state, which can have compounding negative effects on the organism. The cause of death due to B. dendrobatidis, is cardiac arrest due to improper electrolyte blood levels⁹. Mortality rate and time till death by B. dendrobatidis varies with fungal dose, temperature, age of amphibian, and host species^1,10

Bullfrog (Rana catesbeiana) which have been shown to spread Batrachochytrium dendrobatidis http://soundwaves.usgs.gov/2006/01/research.html

Spread
B. dendrobatidis originated in Africa and was transported to other countries through global trade of Xenopus laevis³. Infection has been documented in amphibians from exposure to infected soil or water. B. dendrobatidis can survive in tap water for 3 weeks and deionized water for 4 weeks. In lake water infectivity was observed for 7 weeks after introduction⁴. The bullfrog is the most commonly farmed amphibian and accidental introduction of captive bullfrogs into the wild populations is very common. B. dendrobatidis is asymptomatic in bullfrogs, making it hard to detect without performing tests. B. dendrobatidis has been detected in all introduced species of bullfrogs excluding Japan, and native bullfrogs from Eastern Canada¹¹.

Methods of Control

Example of Didecyldimethylammonium Chloride (DDAC) http://www.avmorgreen.com/english/products.php?cat=16

A number of physical and chemical disinfectants can cause 100% mortality in B. dendrobatidis. The fungus is highly sensitive to heat and died within 4 hours at 37^oC. Desiccation also proved successful. UV light exposure did not kill 100% of the B. dendrobatidis for any of the tested exposure times. Many chemical methods proved useful. Ethanol and Virkon both killed 100% of B. dendrobatidis within 30 seconds. Sodium hyperchlorite was useful, as was Didecyldimethylammonium Chloride (DDAC). DDAC is promoted as being environmentally safe and is used in agriculture and in forestry. Dithane and Formaldehyde were not as effective. Sodium chloride and potassium permanganate were also not as effective¹².

Conclusion

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References

1. Berger, Lee, et al. "Chytridiomycosis causes amphibian mortality associated with population declines in the rain forests of Australia and Central America." Proceedings of the National Academy of Sciences 95.15 (1998): 9031-9036

2. Parris, Matthew J., and Joseph G. Beaudoin. "Chytridiomycosis impacts predator-prey interactions in larval amphibian communities." Oecologia 140.4 (2004): 626-632.

3. Berger, Lee, et al. "Virulence of the amphibian chytrid fungus Batrachochytrium dendrobatidis varies with the strain." Diseases of Aquatic Organisms 68.1 (2005): 47.

4. Johnson, Megan L., and Richard Speare. "Survival of Batrachochytrium dendrobatidis in water: quarantine and disease control implications." Emerging Infectious Diseases 9.8 (2003): 922.

5. Piotrowski, Jeffrey S., Seanna L. Annis, and Joyce E. Longcore. "Physiology of Batrachochytrium dendrobatidis, a chytrid pathogen of amphibians." Mycologia 96.1 (2004): 9-15.

6. Weldon, Ché, et al. "Origin of the amphibian chytrid fungus." Emerging infectious diseases 10.12 (2004): 2100.

7. Longcore, Joyce E., Allan P. Pessier, and Donald K. Nichols. "Batrachochytrium dendrobatidis gen. et sp. nov., a chytrid pathogenic to amphibians." Mycologia (1999): 219-227.

8. Berger, Lee, et al. "Life cycle stages of the amphibian chytrid Batrachochytrium dendrobatidis." Diseases of aquatic organisms 68 (2005): 51-63

9. Voyles, Jamie, et al. "Pathogenesis of chytridiomycosis, a cause of catastrophic amphibian declines." Science 326.5952 (2009): 582-585.

10. Rachowicz, Lara J., and Vance T. Vredenburg. "Transmission of Batrachochytrium dendrobatidis within and between amphibian life stages." Diseases of aquatic organisms 61 (2004): 75-83.

11. Garner, Trenton WJ, et al. "The emerging amphibian pathogen Batrachochytrium dendrobatidis globally infects introduced populations of the North American bullfrog, Rana catesbeiana." Biology Letters 2.3 (2006): 455-459.

12. Johnson, Megan L., et al. "Fungicidal effects of chemical disinfectants, UV light, desiccation and heat on the amphibian chytrid, Batrachochytrium dendrobatidis." Diseases of aquatic organisms 57 (2003): 255-260.

Edited by student of Joan Slonczewski for BIOL 238 Microbiology, 2009, Kenyon College.