Difference between revisions of "Carnobacterium"

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==Cell Structure, Metabolism and Life Cycle==
 
==Cell Structure, Metabolism and Life Cycle==
  
C. divergens & C. maltaromaticum have properties that make them preservative-like. Several strains of C. maltaromaticum  have been FDA approved to be used as a preservative in processed, ready to eat meat products. Species produce lactic acid mainly from glucose. However, C. pleistocenium also produce CO2, ethanol, and acetic acid as well. Most species are psychrophiles, growing at 0 degrees Celsius.  They are gram stain positive, meaning they have a large amount of peptidoglycan in their cell walls. They can be motile or non-motile and do not have spores. There is little known about the metabolism of Carnobacterium, except that they produce lactic acid and get their energy from fermentation of different hexoses.  Acetic acid and ethanol are also common byproducts.  They grow extremely well when heme, a blood pigment with iron, is added to aerobic conditions. However, they can also live in anaerobic conditions as well. C. maltaromaticum and C. mobile may produce gas from glucose, but further research is needed to determine the type of gas and its effect on the environment
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C. divergens & C. maltaromaticum have properties that make them preservative-like. Several strains of C. maltaromaticum  have been FDA approved to be used as a preservative in processed, ready to eat meat products. Species produce lactic acid mainly from glucose. However, C. pleistocenium also produce CO2, ethanol, and acetic acid as well. Most species are psychrophiles, growing at 0 degrees Celsius.  They are gram stain positive, meaning they have a large amount of peptidoglycan in their cell walls. They can be motile or non-motile and do not have spores. There is little known about the metabolism of Carnobacterium, except that they produce lactic acid and get their energy from fermentation of different hexoses.  Acetic acid and ethanol are also common byproducts.  They grow extremely well when heme, a blood pigment with iron, is added to aerobic conditions. However, they can also live in anaerobic conditions as well making them facultive anaerobes. Some interesting finding are that C. maltaromaticum and C. mobile may produce gas from glucose. Also, through the growth of a few Carnobacterium species in food products, volatile alcohols, ketones, and hydrocarbons tend to accumulate. However, Further studies on the importance of these metabolites are needed to determine the to positive and negative effects on the foods.
  
 
==Ecology and Pathogenesis==
 
==Ecology and Pathogenesis==

Revision as of 16:00, 23 April 2014

This student page has not been curated.

Classification

Domain: Bacteria

Phylum: Firmicutes

Class: Bacilli

Order: Lactobacillales

Family: Carnobacteriaceae


Species

Carnobacterium alterfunditum

Carnobacterium divergens

Carnobacterium funditum

Carnobacterium gallinarum

Carnobacterium iners

Carnobacterium inhibens

Carnobacterium jeotgali

Carnobacterium maltaromaticum

Carnobacterium piscicola

Carnobacterium mobile

Carnobacterium pleistocenium

Carnobacterium viridans

  • Please note this is not an exhaustive list.

Description and Significance

Carnobacterium are gram-positive rod-shaped lactic acid bacteria. Although they are lactic acid producing bacteria, they grow in a PH of 9 and do not tolerate acidic environments very well. Most of the species produce lactic acid through various processes. Identifying Carnobacterium is easiest using 16S-23S rDNA ISRs that gives species specific primers that are helpful in distinguishing eight of the Carnobacterium species ( C. divergens, C. mobile, C. funditum, C. alterfun-ditum, C. inhibens, C. viridans, C. gallinarum and C. piscicola) using PCR identification. The presence of Carnobacterium can be found in seawater as well as dairy, fish, & meat products. They are commonly found in in polar regions and temperate environments due to their tolerance to freezing temperatures and thawing. They also are tolerant of high pressure conditions. Carnobacterium include the psycrophilic anaerobic species C. maltaromaticum, C. divergens & C. pleistocenium. Certain species have preservative qualities of meat products. Some are also attributed to meat spoilage. C. pleistocenium has been found in a permafrost fox tunnel in Alaska. The ice dates back to the Pleistocene Epoch (1.8 Ma to 11,000 years ago).


Scanning electron microscopy of Carnobacterium pleistocenium. From Pikuta et al.[7

Genome Structure

Carnobacterium have linear For C. divergens and C. pleistocenium, the genome size was estimated to 3.2 Mb, and for C. alterfunditum, it was estimated to be 2.9 Mb (Daniel, 1995; Pikuta et al., 2005) We only found record of one Carnobacterium genome sequencing project completed. It was Carnobacterium sp. AT7, a piezophilic strain isolated from the Aleutian trench at a depth of 2500 m. The data already available indicate that the Carnobacterium sp. AT7 genome contains 2.4 Mb and encodes 2388 proteins. The final sequence of Carnobacterium sp. 17-4 comprises one chromosome of 2,635,294 bp and one plasmid of 50,105 bp. The chromosome (35.25% G+C content) comprises 2,420 predicted protein-encoding genes, 67 tRNA genes, 8 rRNA operons, and 1 single 5S rRNA gene. The plasmid (31.53% G+C content) harbors 54 protein-encoding genes. (http://www.genomesonline.org) (Liolios et al., 2006).


Table2.jpg

Rachman C, Kabadjova P, Valcheva R, Prévost H & Dousset X (2004) Identification of Carnobacterium species by restriction fragment length polymorphism of the 16S–23S rRNA gene intergenic spacer region and species-specific PCR. Appl Environ Microbiol 70: 4468–4477.

Cell Structure, Metabolism and Life Cycle

C. divergens & C. maltaromaticum have properties that make them preservative-like. Several strains of C. maltaromaticum have been FDA approved to be used as a preservative in processed, ready to eat meat products. Species produce lactic acid mainly from glucose. However, C. pleistocenium also produce CO2, ethanol, and acetic acid as well. Most species are psychrophiles, growing at 0 degrees Celsius. They are gram stain positive, meaning they have a large amount of peptidoglycan in their cell walls. They can be motile or non-motile and do not have spores. There is little known about the metabolism of Carnobacterium, except that they produce lactic acid and get their energy from fermentation of different hexoses. Acetic acid and ethanol are also common byproducts. They grow extremely well when heme, a blood pigment with iron, is added to aerobic conditions. However, they can also live in anaerobic conditions as well making them facultive anaerobes. Some interesting finding are that C. maltaromaticum and C. mobile may produce gas from glucose. Also, through the growth of a few Carnobacterium species in food products, volatile alcohols, ketones, and hydrocarbons tend to accumulate. However, Further studies on the importance of these metabolites are needed to determine the to positive and negative effects on the foods.

Ecology and Pathogenesis

Carnobacterium spp. appear to have both the temperate and polar aquatic environments as habitats including live fish, marine sponges, and Arctic sea water as well as the deep sea. C. maltaromaticum and/or C. divergens have been isolated from tropical fish products, including smoked surubim, a Brazilian tropical freshwater fish, and and from vacuum-packed tuna caught in the Indian Ocean. The presence of carnobacteria has also been documented in the terrestrial environment, including Canadian winter soil and permafrost ice. In these harsh conditions, Carnobacterium possess trials that play a large role in their survival. A cold-active β-galactosidase from C. maltaromaticum and a cold-adapted alanine dehydrogenase from a Carnobacterium sp. related to C. alterfunditum have been found that enhance the Carnobacterium's ability to grow at low temperatures. An example is the isolation of a Carnobacterium sp. preserved in a permafrost ice wedge for 25,000 years. Some carnobacterial isolates are derived from natural high-pressure habitats, as seen in deep Artic water. They are also relatively resistant to high-pressure processing and are found in high concentrations in vacuum-packed and chilled squid mantle and cold-smoked salmon previously treated with 200–400 MPa for 15–20 minutes. There is no research of Carnobacterium causing disease. However, they have been as protective cultures that pathogenic and spoilage microorganisms. Also, C. divergens and C. maltaromaticum are commonly found in the spoilage of chilled seafood and meat products after it has left frozen storage.

References

Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW (2009). GenBank. Nucleic Acids Res. 2009 Jan;37(Database issue):D26-31. Epub 2008 Oct 21. [PubMed]

Collins M.D, Farow J.A.E, Philips B.A, Ferusu S., Jones D. "Classification of Lactobacillus divergens, Lactobacillus piscicola and some catalase-negative, asporogenous, rod-shaped bacteria from poultry in a new genus Carnobacterium" International Journal of Systematic Bacteriology, 37 (1987), pp. 310–316

Joborn A., Dorsch M., Christer O.J., Westerdahl A. & Kjelleberg S. (1999) Carnobacterium inhibens sp. nov., isolated from the intestine of Atlantic salmon (Salmo salar). International Journal of Systematic Bacteriology 49, 1891–1898

Leisner J. J., Laursen B. G., Prevost H., Drider D., Dalgaard P. 2007. "Carnobacterium: positive and negative effects in the environment and in foods." FEMS Microbiol. Rev. 31, 592–613

Manchester, L., Lai, S., and Goodacre, R. "Whole-organism Fingerprinting of the Genus Carnobacterium using Fourier Transform Infrared Spectroscopy (FT-IR)". Systematic and Applied Microbiology . 2004. Volume 27. p. 186-191.

De Vos P., et al. 2009. Bergey's manual of systematic bacteriology, vol. 3 The Firmicutes, p. 549-557. Springer, New York, NY.

Rachman C, Kabadjova P, Valcheva R, Prévost H & Dousset X (2004) Identification of Carnobacterium species by restriction fragment length polymorphism of the 16S–23S rRNA gene intergenic spacer region and species-specific PCR. Appl Environ Microbiol 70: 4468–4477.

Sayers EW, Barrett T, Benson DA, Bryant SH, Canese K, Chetvernin V, Church DM, DiCuccio M, Edgar R, Federhen S, Feolo M, Geer LY, Helmberg W, Kapustin Y, Landsman D, Lipman DJ, Madden TL, Maglott DR, Miller V, Mizrachi I, Ostell J, Pruitt KD, Schuler GD, Sequeira E, Sherry ST, Shumway M, Sirotkin K, Souvorov A, Starchenko G, Tatusova TA, Wagner L, Yaschenko E, Ye J (2009). Database resources of the National Center for Biotechnology Information. Nucleic Acids Res. 2009 Jan;37(Database issue):D5-15. Epub 2008 Oct 21. [PubMed]

Wallbanks, S., A. J. Martinez-Murcia, J. L. Fryer, B. A. Phillips, and M. D. Collins.(1990). "16S RRNA Sequence Determination for Members of the Genus Carnobacterium and Related Lactic Acid Bacteria and Description of Vagococcus Salmoninarum Sp. Nov." International Journal of Systematic Bacteriology 40.3: 224-30

Voget, S., B. Klippel, R. Daniel, and G. Antranikian. "Complete Genome Sequence of Carnobacterium Sp. 17-4. (2011)" Journal of Bacteriology 193.13 :3403-404

Author

Page authored by Heather Moule and Catherine Hencsie, student of Dr. Walker & Dr. Kashefi at Michigan State University.