Chlamydia trachomatis: Difference between revisions
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''Chlamydia trachomatis'' has a genome size that is 1,042,519 base pairs long and has approximately 894 protein coding sequences. ''C. trachomatis'' strains have an extrachromosomal plasmid. | ''Chlamydia trachomatis'' has a genome size that is 1,042,519 base pairs long and has approximately 894 protein coding sequences. ''C. trachomatis'' strains have an extrachromosomal plasmid. ll plasmids from human C. trachomatis isolates are extremely similar, with less than 1% nucleotide sequence variation All are about 7,500 nucleotides in size, with eight open reading frames computer-predicted to code for proteins of more than 100 amino acids, with short non-coding sequences between some of them only | ||
In their nucleotide sequence, chlamydial plasmids are more closely related than is the corresponding chromosomal DNA | |||
The chlamydial plasmid has great practical importance. It is a favoured target for DNA-based diagnosis of C. trachomatis infection for two reasons. Firstly, there are approximately 7-10 copies of the plasmid present per chlamydial particle. Its sequence is highly conserved among different isolates of C. trachomatis. There are authenticated examples of C. trachomatis strains lacking the plasmid and it does have effects. Plaque purified C. trachomatis free of the plasmid has unusual inclusion morphology, is glycogen free, and shows no alteration in antibiotic sensitivity . However, the fact that such strains exist shows that the plasmid is not essential for C. trachomatis survival. | |||
==Cell structure and metabolism== | ==Cell structure and metabolism== | ||
Revision as of 07:17, 1 May 2007
Classification
Higher order taxa
Archaea; Euryarchaeota; Thermoplasmata; Thermoplasmatales; Thermoplasmataceae; Thermoplasma
Genus
Thermoplasma volcanium GSS1
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NCBI: Taxonomy |
Description and significance
Describe the appearance, habitat, etc. of the organism, and why it is important enough to have its genome sequenced. Describe how and where it was isolated. Include a picture or two (with sources) if you can find them.
The chlamydial outer membrane complex is composed primarily of three proteins; the major outer membrane protein (MOMP) and two cysteine-rich proteins, the outer membrane complex B protein (OmcB) and the outer membrane complex A protein (OmcA). Chlamydial major outer membrane protein, which by reason of its importance is the most extensively studied, was discovered in 1981 by three independent laboratories
SDS - PAGE electrophoresis
Genome structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Does it have any plasmids? Are they important to the organism's lifestyle?
Chlamydia trachomatis has a genome size that is 1,042,519 base pairs long and has approximately 894 protein coding sequences. C. trachomatis strains have an extrachromosomal plasmid. ll plasmids from human C. trachomatis isolates are extremely similar, with less than 1% nucleotide sequence variation All are about 7,500 nucleotides in size, with eight open reading frames computer-predicted to code for proteins of more than 100 amino acids, with short non-coding sequences between some of them only
In their nucleotide sequence, chlamydial plasmids are more closely related than is the corresponding chromosomal DNA
The chlamydial plasmid has great practical importance. It is a favoured target for DNA-based diagnosis of C. trachomatis infection for two reasons. Firstly, there are approximately 7-10 copies of the plasmid present per chlamydial particle. Its sequence is highly conserved among different isolates of C. trachomatis. There are authenticated examples of C. trachomatis strains lacking the plasmid and it does have effects. Plaque purified C. trachomatis free of the plasmid has unusual inclusion morphology, is glycogen free, and shows no alteration in antibiotic sensitivity . However, the fact that such strains exist shows that the plasmid is not essential for C. trachomatis survival.
Cell structure and metabolism
Describe any interesting features and/or cell structures; how it gains energy; what important molecules it produces.
Ecology
Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.
Pathology
How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
Application to Biotechnology
Does this organism produce any useful compounds or enzymes? What are they and how are they used?
Current Research
Enter summaries of the most recent research here--at least three required
References
example:
Edited by student of Rachel Larsen and Kit Pogliano
