Deinococcus radiodurans NEU2011: Difference between revisions
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Deinococcus radiodurans is a spherical bacteria 1-2 μm in diameter that tends to form tetrads and is extremely resistant to radiation. It is red in color and non-motile as well as nonsporulating. It is found in many places worldwide, from soil, elephant dung, and granite in Antarticas dry valleys. It was first isolated in 1956 when canned meat that spoiled even though it had been exposed to radiation. | Deinococcus radiodurans is a spherical bacteria 1-2 μm in diameter that tends to form tetrads and is extremely resistant to radiation. It is red in color and non-motile as well as nonsporulating. It is found in many places worldwide, from soil, elephant dung, and granite in Antarticas dry valleys. It was first isolated in 1956 when canned meat that spoiled even though it had been exposed to radiation. | ||
Another unique feature of D. radiodurans is that it has multiple copies of its genome. During the exponential growth phase D. radiodurans will have 8-10 copies of its genome. During the stationary growth phase it will still have 4 copies of its genome, which consists of 2 chromosomes, 1 megaplasmid, and 1 plasmind. | |||
Deinococcus radiodurans is highly resistant against Ionizing radiation, Ultraviolet radiation, oxidative stress, and desiccation. Although radiation causes a delay in growth and replication briefly D. radiodurans can recover from 15kGy of gamma radiation, a level that is deadly to most life. D. radiodurans is 50 times more resistant than E. coli to ionizing radiation and about 33 times more resistant than E. coli to ultraviolet radiation. It is no less susceptible to the damage that radiation causes than other cells, but is however much better at repairing its genome when radiation causes double stranded breaks (DSB). E. coli can withstand about 10-15 DSB’s, while D. radiodurans is able to repair 1,000-2,000 DSB’s. It is theorized tht perhaps D. radiodurans is able to use the multiple copies of its genomes to find templates for repair when mending DSB’s. Many people wondered what selective pressure caused D. radiodurans to develop such extreme resilience to radiation when no such conditions are present on earth. It is postulated that it was severe dehydration, which can cause similar DNA damage to radiation was the pressure that lead to the evolution of D. radiodurans. | Deinococcus radiodurans is highly resistant against Ionizing radiation, Ultraviolet radiation, oxidative stress, and desiccation. Although radiation causes a delay in growth and replication briefly D. radiodurans can recover from 15kGy of gamma radiation, a level that is deadly to most life. D. radiodurans is 50 times more resistant than E. coli to ionizing radiation and about 33 times more resistant than E. coli to ultraviolet radiation. It is no less susceptible to the damage that radiation causes than other cells, but is however much better at repairing its genome when radiation causes double stranded breaks (DSB). E. coli can withstand about 10-15 DSB’s, while D. radiodurans is able to repair 1,000-2,000 DSB’s. It is theorized tht perhaps D. radiodurans is able to use the multiple copies of its genomes to find templates for repair when mending DSB’s. Many people wondered what selective pressure caused D. radiodurans to develop such extreme resilience to radiation when no such conditions are present on earth. It is postulated that it was severe dehydration, which can cause similar DNA damage to radiation was the pressure that lead to the evolution of D. radiodurans. | ||
D. radiodurans has been of fascination to researchers who are eager to understand its amazing DNA repair mechanisms. It also has been of useful as a genetically engineered bacteria. It has been recombined to be capable of metabolizing toxic toluene and mercury from radioactive waste, as it can survive in the extreme radioactive conditions. | D. radiodurans has been of fascination to researchers who are eager to understand its amazing DNA repair mechanisms. It also has been of useful as a genetically engineered bacteria. It has been recombined to be capable of metabolizing toxic toluene and mercury from radioactive waste, as it can survive in the extreme radioactive conditions. | ||
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The genome contains 3187 open reading frames (ORFs), with an average size of 937 bp, representing 91% of the genome.Analysis of the genome shows that almost 30% of the total number of genes encodes proteins with regulatory functions (39 of 140) including: transcription factors, response regulators, and kinases are found on chromosome II and the megaplasmid. | The genome contains 3187 open reading frames (ORFs), with an average size of 937 bp, representing 91% of the genome.Analysis of the genome shows that almost 30% of the total number of genes encodes proteins with regulatory functions (39 of 140) including: transcription factors, response regulators, and kinases are found on chromosome II and the megaplasmid. | ||
Cell Structure and Metabolism | |||
Deinococcus Radiodurans is a obligate aerobe that is chemoorganotrophic (Battista). This bacterium however has been recombined in order for it to be able to metabolize various radioactive toxic waste, such as toluene and mercury. It is cocci shape bacterium that stains gram positive although it’s cell structure more closely resembles that of a gram negative cell. It has an outer and an inner membrane and a thicker peptidoglycan layer that is 14-20nm thick. On top of the peptidoglycan layer there is another layer that is broken up into many small compartments. | |||
References: | References: | ||
-Battista JR. Against all odds: the survival strategies of Deinococcus radiodurans. Annu Rev Microbiol. 1997;51:203-24. Review. PubMed PMID: 9343349. | |||
-Makarova K, Minton K, et al. Genome of the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans Viewed from the Perspective of Comparative Genomics. Microbiology and Molecular Biology Reviews. March 2001. Volume 65. p. 44-79. | -Makarova K, Minton K, et al. Genome of the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans Viewed from the Perspective of Comparative Genomics. Microbiology and Molecular Biology Reviews. March 2001. Volume 65. p. 44-79. | ||
- White O, Eisen JA, Heidelberg JF, Hickey EK, et al. Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1. | - White O, Eisen JA, Heidelberg JF, Hickey EK, et al. Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1. | ||
Science. November; 1999. 19;286(5444):1571-7. | Science. November; 1999. 19;286(5444):1571-7. | ||
-http://bioweb.uwlax.edu/bio203/s2008/arnold_meag/Phylogeny.htm | -http://bioweb.uwlax.edu/bio203/s2008/arnold_meag/Phylogeny.htm | ||
Revision as of 04:02, 31 March 2011
Deinococcus radiodurans
Classifications
Higher Taxa
Kingdom: Bacteria
Phylum: Deinococcus-Thermus
Order: Deinococcales
Family: Deinococcaaceae
Genus: Deinococcus
Species: radiodurans
Description and Significance
Deinococcus radiodurans is a spherical bacteria 1-2 μm in diameter that tends to form tetrads and is extremely resistant to radiation. It is red in color and non-motile as well as nonsporulating. It is found in many places worldwide, from soil, elephant dung, and granite in Antarticas dry valleys. It was first isolated in 1956 when canned meat that spoiled even though it had been exposed to radiation. Another unique feature of D. radiodurans is that it has multiple copies of its genome. During the exponential growth phase D. radiodurans will have 8-10 copies of its genome. During the stationary growth phase it will still have 4 copies of its genome, which consists of 2 chromosomes, 1 megaplasmid, and 1 plasmind. Deinococcus radiodurans is highly resistant against Ionizing radiation, Ultraviolet radiation, oxidative stress, and desiccation. Although radiation causes a delay in growth and replication briefly D. radiodurans can recover from 15kGy of gamma radiation, a level that is deadly to most life. D. radiodurans is 50 times more resistant than E. coli to ionizing radiation and about 33 times more resistant than E. coli to ultraviolet radiation. It is no less susceptible to the damage that radiation causes than other cells, but is however much better at repairing its genome when radiation causes double stranded breaks (DSB). E. coli can withstand about 10-15 DSB’s, while D. radiodurans is able to repair 1,000-2,000 DSB’s. It is theorized tht perhaps D. radiodurans is able to use the multiple copies of its genomes to find templates for repair when mending DSB’s. Many people wondered what selective pressure caused D. radiodurans to develop such extreme resilience to radiation when no such conditions are present on earth. It is postulated that it was severe dehydration, which can cause similar DNA damage to radiation was the pressure that lead to the evolution of D. radiodurans. D. radiodurans has been of fascination to researchers who are eager to understand its amazing DNA repair mechanisms. It also has been of useful as a genetically engineered bacteria. It has been recombined to be capable of metabolizing toxic toluene and mercury from radioactive waste, as it can survive in the extreme radioactive conditions.
Genome structure:
The genome is composed of four circular molecules:
- Chromosome I = 2,648,638 base pairs (bp)
- Chromosome II = 412,348 base pairs
- A megaplasmid = 177,466 base pairs
- A plasmid = 45,704 base pairs
The genome contains 3187 open reading frames (ORFs), with an average size of 937 bp, representing 91% of the genome.Analysis of the genome shows that almost 30% of the total number of genes encodes proteins with regulatory functions (39 of 140) including: transcription factors, response regulators, and kinases are found on chromosome II and the megaplasmid.
Cell Structure and Metabolism
Deinococcus Radiodurans is a obligate aerobe that is chemoorganotrophic (Battista). This bacterium however has been recombined in order for it to be able to metabolize various radioactive toxic waste, such as toluene and mercury. It is cocci shape bacterium that stains gram positive although it’s cell structure more closely resembles that of a gram negative cell. It has an outer and an inner membrane and a thicker peptidoglycan layer that is 14-20nm thick. On top of the peptidoglycan layer there is another layer that is broken up into many small compartments.
References:
-Battista JR. Against all odds: the survival strategies of Deinococcus radiodurans. Annu Rev Microbiol. 1997;51:203-24. Review. PubMed PMID: 9343349. -Makarova K, Minton K, et al. Genome of the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans Viewed from the Perspective of Comparative Genomics. Microbiology and Molecular Biology Reviews. March 2001. Volume 65. p. 44-79. - White O, Eisen JA, Heidelberg JF, Hickey EK, et al. Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1. Science. November; 1999. 19;286(5444):1571-7. -http://bioweb.uwlax.edu/bio203/s2008/arnold_meag/Phylogeny.htm
