File:Chimera Diagram.jpg: Difference between revisions
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(Construction of ChimeriVax-based vaccines. Chimeric flavivirus vaccines are constructed by replacing the genes coding for prM and E proteins from YFV 17D 204 vaccine with those of heterologous flaviviruses (DEN, WN or JE). After DNA cloning, RNA is tra...) |
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Latest revision as of 19:21, 8 May 2014
Construction of ChimeriVax-based vaccines. Chimeric flavivirus vaccines are constructed by replacing the genes coding for prM and E proteins from YFV 17D 204 vaccine with those of heterologous flaviviruses (DEN, WN or JE). After DNA cloning, RNA is transcribed and transfected into Vero cells to obtain chimeric viruses possessing the YFV 17D replication machinery and the external coat of the relevant heterologous flavivirus.
dx.doi.org/10.1016/j.vaccine.2009.09.098
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