Gisella Alcaraz-Bacillus Pumilus

Classification

• Domain: Bacteria

• Kingdom: Eubacteria

• Phylum: Firmicutes

• Class: Bacilli

• Order: Bacillales

• Family: Bacillaceae

Species

• Genus: Bacillis

• Species: Pumilus

Habitat Information

Describe the location and conditions under which the organism was isolated.

Description and Significance

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.

• Colony Morphology:

• Cellular Morphology:

• When spread on a plate of Gram (+) E. faecalis, it produced a slight zone of inhibition.

• Organism may be significant because of ________________________________________________________________________________

Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.

Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.

Physiology and Pathogenesis

Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

Further testing and Results from Biochemical Tests:

MR-VP (Methyl Red and Voges-Proskauer)Tests:

• No mixed acid fermentation
• Neutral end products.

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Citrate Test:

• Organism can use Citrate as the only carbon source.

SIM Test:

• Cannot use Sulfur as terminal electron acceptor
• Organism is not motile
• Tryptophan is not broken down and indole is not produced.

Nitrate Test:

• Organism did not make NO2 from NO3 OR organism broke down NO3 into another nitrogenous product.
• After adding zinc solution turned red = Organism did not reduce nitrate.

Urea Test:

• Organism is not able to break down Urea to Ammonia

TSI Test:

• Red Slant/ Yellow Butt/No gas production

-Does not ferment either lactose or sucrose -Some fermentation has occurred, acid has been produced, it is a facultative anaerobe.

Decarboxylation Test:

• Arginine- Negative for Arginine decarboxylase
• Lysine- Negative for Lysine decarboxylase
• Ornithine- Negative for Ornithine decarboxylase

Phenylaline Deaminase Test:

• Tested negative, which means that this organism is not able to remove amino group from phenylalnine.

Oxidase Test :

• (-) Organism is anaerobic or facultative anaerobic.

Eosin Methyline Blue Agar (EMB):

• No growth of organism, which makes this test lead us to the conclusion that the organism is Gram Positive.

Hektoen Enteric Agar (HE):

• Determind it was a Gram Positive.
• Negative for lactose fermentation.

MacConkey Agar (MAC):

• No growth, agrees with EMB test.
• Positive for lactose fermentation.

Blood Agar Test:

• β- complete breakdown of Nutrient Agar.

Mannitol Salt Agar (MSA):

• Organism is Gram Positive.
• Negative for fermentation.

Phenylethyl Alcohol Agar (PEA):

• Growth on plate leads us to the same conclusions that the organism is Gram Positive.

Catalase Test:

• Organism is able to take hydrogen peroxide and make it less harmful.
• Tested positive for catalase.

6.5% Salt Tolerance Test:

• There was growth in the broth.

Bile Esculin Test:

• Tested positive, it is able to hydrolyze Esculin in the presence of bile.

References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Author

Page authored by Gisella Alcaraz, student of Prof. Kristine Hollingsworth at Austin Community College.