Jamie and Erika's Bacteria: Difference between revisions
Jamie.ball (talk | contribs) |
Jamie.ball (talk | contribs) |
||
| (22 intermediate revisions by the same user not shown) | |||
| Line 4: | Line 4: | ||
Because our PCR and gel electrophoresis were inconclusive, we were not able to perform a BLAST search for our DNA sequence and we are not 100% sure what bacteria it is. | Because our PCR and gel electrophoresis were inconclusive, we were not able to perform a BLAST search for our DNA sequence and we are not 100% sure what bacteria it is. | ||
Our DNA Hydrolysis test was positive (showed clearing) so we can assume that the bacteria contains DNA but we made a mistake during the electrophoresis loading procedures. | |||
From our test results, We can most likely eliminate Streptococcus, Staphlococcus, and Enterobactereae. | |||
===Species=== | ===Species=== | ||
| Line 12: | Line 15: | ||
|} | |} | ||
''Genus species'' Unknown | ''Genus species'': Unknown | ||
==Habitat Information == | ==Habitat Information == | ||
Our organism was found at 30.2370 degrees N, 97.7043 degrees W. according to the grid from NRCS it was found at 3345800 x 624520. It was obtained about an inch and a half from the surface, the soil was dry and grainy. Only a small amount of grass and one tree were close to the collection site. | |||
Our soil area was believed to contain Tin clay, Patrick soils, Lewisville silty clay, Houston Black soil, Chaney fine sandy loam and Altoga silty clay. | |||
The sample was collected on September 8th of 2017, the temperature was estimated to be between 57 and 84 degrees Farenheit. Humidity was at 54% and 24 hour rainfall was 0.00 inches. The pressure was recorded at 29.87 alt (in) or 1010.4 sea level (m/lb) and solar radiation was 22.45 mj/m2 | |||
==Description and Significance== | ==Description and Significance== | ||
Cellular Morphology: The bacteria is a Gram-negative bacillus that showed mostly singular bacilli and a few diplobacillus throughout. It is non-motile. Our capsule stain was negative and the Endospore stain was positive. | |||
Colony Morphology: The bacteria presented entire margins, creamy white, slightly yellow/tan, opaque, shiny in appearance with no extracellular staining. Isolated colonies were round, moist looking and slightly raised. This bacterium did present a slight odor. | |||
The bacteria shoed no sensitivity to Bacitracin or Optichin disks, it grew as normal in the agar. It was also tested for a sensitivity to Axlocillin, Ceftazidines, Ampicillin, Oxacillin, and Vancomycin. Out of these five, only Ceftazidine presented a zone of inhibition to the growth of the bacteria. Vancomycin showed a very slight zone of inhibition, but probably not enough to be significant in fighting the bacteria. | |||
==Genome Structure== | ==Genome Structure== | ||
Since our PCR and gel electrophoresis techniqes were inconclusive, we cant be sure what our genome structure consists of. Since it is a bacteria we can assume it would have a circular chromosome. | |||
==Cell Structure, Metabolism and Life Cycle== | |||
Since our bacteria was positive for the Oxidase test we can assume it uses the electron transport chain for energy. It also appears to be aerobic. | |||
==Physiology and Pathogenesis== | |||
Selective Media Test Results: | |||
Hektoen Enteric Agar = Negative / No Growth | |||
== | MacConkey Agar = Negative / No Growth | ||
Mannitol Salt Agar = Negative / No Growth | |||
6.5% Salt Broth = Negative / No Growth | |||
Phenylethyl Alcohol Agar = Negative / No Growth | |||
Eosin Methylene Blue Agar = Positive for growth, mucoid pink growth indicates weak lactose fermentation | |||
Differential Tests: | |||
Blood Agar = Gamma Hemolysis (no clearing) | |||
Nitrate Reduction = Negative for Nitrate reduction abilities | |||
Triple Sugar Iron Agar = No fermentation / No gas production | |||
Citrate Test = Negative | |||
MR VP = Negative / Negative | |||
Phenol Red Broth = No fermentation / No gas | |||
Decarboxylation Test = Negative for all three substrates | |||
Phenylalanine Deaminase = Negative | |||
SIM Test = Negative for motility / Negative for tryptophanase / Negative sulfur reduction | |||
Catalase Test = Postive | |||
Oxidase Test = Positive | |||
Bile Esculine = Positive | |||
Gelatin Hydrolysis = Negative | |||
Lipid Hydrolysis = Positive | |||
Starch Hydrolysis = Negative | |||
DNA Hydrolysis = Positive | |||
Urea Hydrolysis = Negative | |||
No known enzymes as of now.No known contributions to the environment as of now. | |||
Diseases and symptoms unknown as of right now. | |||
<br><br> | |||
==References== | ==References== | ||
None yet. | |||
==Author== | ==Author== | ||
Page authored by | Page authored by Jamie Powell and Erika Anderson, students of Prof. Kristine Hollingsworth at Austin Community College. | ||
<!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]] | <!-- Do not remove this line-->[[Category:Pages edited by students of Kristine Hollingsworth at Austin Community College]] | ||
Latest revision as of 17:48, 4 December 2017
Classification
Because our PCR and gel electrophoresis were inconclusive, we were not able to perform a BLAST search for our DNA sequence and we are not 100% sure what bacteria it is.
Our DNA Hydrolysis test was positive (showed clearing) so we can assume that the bacteria contains DNA but we made a mistake during the electrophoresis loading procedures.
From our test results, We can most likely eliminate Streptococcus, Staphlococcus, and Enterobactereae.
Species
|
NCBI: Taxonomy |
Genus species: Unknown
Habitat Information
Our organism was found at 30.2370 degrees N, 97.7043 degrees W. according to the grid from NRCS it was found at 3345800 x 624520. It was obtained about an inch and a half from the surface, the soil was dry and grainy. Only a small amount of grass and one tree were close to the collection site.
Our soil area was believed to contain Tin clay, Patrick soils, Lewisville silty clay, Houston Black soil, Chaney fine sandy loam and Altoga silty clay.
The sample was collected on September 8th of 2017, the temperature was estimated to be between 57 and 84 degrees Farenheit. Humidity was at 54% and 24 hour rainfall was 0.00 inches. The pressure was recorded at 29.87 alt (in) or 1010.4 sea level (m/lb) and solar radiation was 22.45 mj/m2
Description and Significance
Cellular Morphology: The bacteria is a Gram-negative bacillus that showed mostly singular bacilli and a few diplobacillus throughout. It is non-motile. Our capsule stain was negative and the Endospore stain was positive.
Colony Morphology: The bacteria presented entire margins, creamy white, slightly yellow/tan, opaque, shiny in appearance with no extracellular staining. Isolated colonies were round, moist looking and slightly raised. This bacterium did present a slight odor.
The bacteria shoed no sensitivity to Bacitracin or Optichin disks, it grew as normal in the agar. It was also tested for a sensitivity to Axlocillin, Ceftazidines, Ampicillin, Oxacillin, and Vancomycin. Out of these five, only Ceftazidine presented a zone of inhibition to the growth of the bacteria. Vancomycin showed a very slight zone of inhibition, but probably not enough to be significant in fighting the bacteria.
Genome Structure
Since our PCR and gel electrophoresis techniqes were inconclusive, we cant be sure what our genome structure consists of. Since it is a bacteria we can assume it would have a circular chromosome.
Cell Structure, Metabolism and Life Cycle
Since our bacteria was positive for the Oxidase test we can assume it uses the electron transport chain for energy. It also appears to be aerobic.
Physiology and Pathogenesis
Selective Media Test Results:
Hektoen Enteric Agar = Negative / No Growth
MacConkey Agar = Negative / No Growth
Mannitol Salt Agar = Negative / No Growth
6.5% Salt Broth = Negative / No Growth
Phenylethyl Alcohol Agar = Negative / No Growth
Eosin Methylene Blue Agar = Positive for growth, mucoid pink growth indicates weak lactose fermentation
Differential Tests:
Blood Agar = Gamma Hemolysis (no clearing)
Nitrate Reduction = Negative for Nitrate reduction abilities
Triple Sugar Iron Agar = No fermentation / No gas production
Citrate Test = Negative
MR VP = Negative / Negative
Phenol Red Broth = No fermentation / No gas
Decarboxylation Test = Negative for all three substrates
Phenylalanine Deaminase = Negative
SIM Test = Negative for motility / Negative for tryptophanase / Negative sulfur reduction
Catalase Test = Postive
Oxidase Test = Positive
Bile Esculine = Positive
Gelatin Hydrolysis = Negative
Lipid Hydrolysis = Positive
Starch Hydrolysis = Negative
DNA Hydrolysis = Positive
Urea Hydrolysis = Negative
No known enzymes as of now.No known contributions to the environment as of now.
Diseases and symptoms unknown as of right now.
References
None yet.
Author
Page authored by Jamie Powell and Erika Anderson, students of Prof. Kristine Hollingsworth at Austin Community College.
