Lactobacillus camelliae

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Classification

Domain: Bacteria Kingdom: Bacteria Phylum: Firmicutes Class: Bacilli Order: Lactobacillales Family: Lactobacillaceae Genus: Lactobacillus Species: Lactobacillus camelliae

Genus Species

Lactobacillus camelliae

Description and Significance

Lactobacillus, also called Döderlein's bacillus, is a genus of Gram-positive facultative anaerobic or microaerophilic rod-shaped bacteria. [1] Lactobacillus camelliea is the species of Lactobacillus which found in camelliea leaves.

Eighteen rod-shaped homofermentatives, six heterofermentatives, and a coccal homofermentative lactic acid bacteria were isolated from fermented tea leaves (miang) produced in the northern part of Thailand. The isolates were placed in a monophyletic cluster consisting of Lactobacillus and Pediococcus species. They were divided into seven groups by phenotypic and chemotaxonomic characteristics, DNA-DNA similarity, and 16S rRNA gene sequences. Groups I to VI belonged to Lactobacillus and Group VII to Pediococcus. All of the strains tested produced DL-lactic acid but those in Group IV produced L-lactic acid. The strains tested in Groups I, II and V had meso-diaminopimelic acid in the cell wall. Six strains in Group I were identified as Lactobacillus pantheris; five strains in Group II as Lactobacillus pentosus; and four strains in Group V as Lactobacillus suebicus. Two strains in Group VI showed high DNA-DNA similarity for each other and MCH4-2 was closest to Lactobacillus fermentum CECT 562(T) with 99.5% of 16S rRNA gene sequence similarity. Five strains in Group III are proposed as Lactobacillus thailandensis sp. nov., and MCH5-2(T) (BCC 21235(T)=JCM 13996(T)=NRIC 0671(T)=PCU 272(T)) is the type strain which has 49 mol% G+C of DNA. Two strains in Group IV are proposed as Lactobacillus camelliae sp. nov., and the type strain is MCH3-1(T) (BCC 21233(T)=JCM 13995(T)=NRIC 0672(T)=PCU 273(T)) which has 51.9 mol% G+C of DNA. One strain in Group VII is proposed as Pediococcus siamensis sp. nov., and MCH3-2(T) (BCC 21234(T)=JCM 13997(T)=NRIC 0675(T)=PCU 274(T)) is the type strain which has 42 mol% G+C of DNA.

Location/Qualifiers
    source          1..1530
                    /organism="Lactobacillus camelliae"
                    /mol_type="rRNA"
                    /strain="MCH3-1"
                    /isolation_source="fermented tea leaves (miang)"
                    /db_xref="taxon:381742"
                    /note="type strain of Lactobacillus camelliae"
    rRNA            1..1530
                    /product="16S ribosomal RNA"

Lactobacillus camelliae strain MCH3-1 16S ribosomal RNA, complete

           sequence.

Structure, Metabolism, and Life Cycle

Interesting features of its structure; how it gains energy (how it replicates, if virus); what important molecules it produces (if any), does it have an interesting life cycle?

Ecology and Pathogenesis

Natural habitat (soil, water, commensal of humans or animals?)
If relevant, how does this organism cause disease? Human, animal, or plant hosts? Important virulence factors, as well as patient symptoms. A Gram-positive and catalase-negative coccus that formed chains, strain FP15-1(T), isolated from fermented tea leaves ('miang'), was studied systematically.

References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1622870/] Tanasupawat, S., Pakdeeto, A., Thawai, C., Yukphan, P., and Okada, S. Identification of lactic acid bacteria from fermented tea leaves (miang) in Thailand and proposals of Lactobacillus thailandensis sp. nov., Lactobacillus camelliae sp. nov., and Pediococcus siamensis sp. nov." J. Gen. Appl. Microbiol. (2007) 53:7-15.

Author

Page authored by __Kaiwen Huang___, student of Mandy Brosnahan, Instructor at the University of Minnesota-Twin Cities, MICB 3301/3303: Biology of Microorganisms.