Methylococcus capsulatus

A Microbial Biorealm page on the genus Methylococcus capsulatus

Classification

Higher order taxa

Kingdom: Bacteria Domain: Proteobacteria Phylum: Gammaproteobacteria Class: Methylococcales Order: Methylococcaceae Family: Methylococcus

Species

1. Genus: Methylococcus

1. Species: capsulatus

Description and significance

Methylococcus capsulatus is a methylotrophic “Gram-negative” bacterium with coccus shape, live in multiple habitats, however, oxygen is a necessity for such cell to survive. Methylococcus capsulatus is also a thermophilic microbe which optically live in the temperature of 45C. Methylococcus capsulatus is isolated from Methanotrophic bacterium, which serves a unique biological function in earth's environment. Methanotrophic bacteria is able to generate Green House gas, Methane, as an energy source for growth (detail mechanism refer to section, cell structure and metabolism), and contribute to lower the methane level in earth's atmosphere.

Genome structure

The Complete genome of M. capsulatus (Bath) Genome is consisted with 3,304,697 base pairs along with 63.6% of G,C contents. The total number of CDSs (predicted coding sequence) is 3,120 with the average size of 962 base pairs. The number of rRNA operons (16S-23S-5S) in M. capsulatus (Bath) Genome is 2, the number of tRNA genes is 46 and the number of sRNA genes is 3. 1,766 proteins of known function and role category and 109 proteins of known function but unknown role category were discovered that serves similar function to M. capsulatus (Bath).

Cell structure and metabolism

"Methylococcus capsulatus is a methanotroph (Methane-oxidising bacteria.) Methanotrophs are ubiquitous Gram-negative bacteria that can use the greenhouse gas methane as a sole carbon and energy source for growth, thus playing major roles in global carbon cycles, and in particular, substantially reducing emissions of biologically generated methane to the atmosphere."

"Methanotrophs are also able to metabolize or co-metabolize xenobiotic compounds, including chlorinated solvents such as trichloroethylene, and hence have potential as bioremediation tools."

Ecology

In the article, “Scientists explore genome of methane-breathing microbe” released on September 20th, 2004 by Robert Koenig, the genome sequence of Methylococcus capsulatus was found useful to study “methane-fixing symboints,” because majority of such organisms are usually discovered inside of live animals and cannot be grown on pure culture. Inherited the characteristic of Methanotrophs, M. Capsulatus contributes to the earth's ecosystem greatly by consuming methane, a product derived from chemical process of landfills and in the guts of ruminant livestock and also by many oil and gas processing plants.

Pathology

The research of disease cause by Methylococcus capsulatus is still in discovering.

Application to Biotechnology

Methylococcus capsulatus Bath has in common with all of the genomes in the CMR (not including itself), based on all v/s all searches. Each dot on the plot below represents a "match" organism in the CMR. The X-axis value is the total number of genes in each match organism. The Y-axis value is the number of "Best Protein Hits" between the match organism and Methylococcus capsulatus Bath, based on the all v/s all blast searches. The "Best Protein Hit" for each Methylococcus capsulatus Bath protein is determined by the protein or proteins from the blast search that have the lowest P value. Below the graph is a table listing all of the organisms in the CMR and the number of "Best Protein Hits" between the match organism and Methylococcus capsulatus Bath.

Current Research

Arch Microbiol. 2005 Nov 26; :1-16 16311759   	

Analysing the outer membrane subproteome of Methylococcus capsulatus (Bath) using proteomics and novel biocomputing tools. Frode Berven , Odd Karlsen , Anne Straume , Kristian Flikka , J Murrell , Anne Fjellbirkeland , Johan Lillehaug , Ingvar Eidhammer , Harald Jensen High-resolution two-dimensional gel electrophoresis and mass spectrometry has been used to identify the outer membrane (OM) subproteome of the Gram-negative bacterium Methylococcus capsulatus (Bath). Twenty-eight unique polypeptide sequences were identified from protein samples enriched in OMs. Only six of these polypeptides had previously been identified. The predictions from novel bioinformatic methods predicting beta-barrel outer membrane proteins (OMPs) and OM lipoproteins were compared to proteins identified experimentally. BOMP ( http://www.bioinfo.no/tools/bomp ) predicted 43 beta-barrel OMPs (1.45%) from the 2,959 annotated open reading frames. This was a lower percentage than predicted from other Gram-negative proteomes (1.8-3%). More than half of the predicted BOMPs in M. capsulatus were annotated as (conserved) hypothetical proteins with significant similarity to very few sequences in Swiss-Prot or TrEMBL. The experimental data and the computer predictions indicated that the protein composition of the M. capsulatus OM subproteome was different from that of other Gram-negative bacteria studied in a similar manner. A new program, Lipo, was developed that can analyse entire predicted proteomes and give a list of recognised lipoproteins categorised according to their lipo-box similarity to known Gram-negative lipoproteins ( http://www.bioinfo.no/tools/lipo ). This report is the first using a proteomics and bioinformatics approach to identify the OM subproteome of an obligate methanotroph.

Reference

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Edited by Ting Yuan Feng, student of Rachel Larsen at UCSD.Sci. U. S. A.