Methylothermus thermalis: Difference between revisions

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Gram-negative, non-motile coccoids 0.6-0.8 micrometers in diameter. Describe the appearance, habitat, etc. of the organism, and why you think it is important.
Gram-negative, non-motile coccoids 0.6-0.8 micrometers in diameter. Describe the appearance, habitat, etc. of the organism, and why you think it is important.


[[Image:Phyoshit.jpg|frame|]]
==Genome Structure==
==Genome Structure==
the nifH gene was not detected with PCR analysis. Also, the mmoX gene was not detected by PCR using the respective primer set that is universal for the known mmoX genes of methanotrophs. Describe the size and content of the genome.  How many chromosomes?  Circular or linear?  Other interesting features?  What is known about its sequence?
the nifH gene was not detected with PCR analysis. Also, the mmoX gene was not detected by PCR using the respective primer set that is universal for the known mmoX genes of methanotrophs. Describe the size and content of the genome.  How many chromosomes?  Circular or linear?  Other interesting features?  What is known about its sequence?

Revision as of 03:37, 21 April 2008

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Classification

Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae

Species

Methylothermus thermalis

NCBI: Taxonomy

Description and Significance

Gram-negative, non-motile coccoids 0.6-0.8 micrometers in diameter. Describe the appearance, habitat, etc. of the organism, and why you think it is important.

Phyoshit.jpg

Genome Structure

the nifH gene was not detected with PCR analysis. Also, the mmoX gene was not detected by PCR using the respective primer set that is universal for the known mmoX genes of methanotrophs. Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence?

Pathways for the oxidation of methane and assimilation of formaldehyde. Abbreviations: CytC, cytochrome c; FADH, formaldehyde dehydrogenase; FDH,formate dehydrogenase. From https://www.ocean.udel.edu/cms/thanson/TH-MicroRev.pdf Hanson R and Hanson T, 1996.].
RuMP pathway for formaldehyde fixation. The reactions catalyzed by the unique enzymes of this pathway, hexulose-6-phosphate synthase and hexulosephosphate isomerase, are indicated. From https://www.ocean.udel.edu/cms/thanson/TH-MicroRev.pdf Hanson R and Hanson T, 1996.].

Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.








Ecology

Japanese Hot Spring; Require methane or methanol as a carbon source to growth. Can use nitrate, ammonia, urea, tryptophan, lysine, glutamate, formamide and Triss as nitrogen sources, but no growth was obsevered without one of these sources. symbiosis; biogeochemical significance; contributions to environment.



Characteristics of type I, type II, and type X methanotrophs; phylogenetic signature probes 1041 and 1035 will not hyidize with 16S rRNAs from members of the genus Methylomonas. Probes MM650 and MM850 have been employed to detect some species in this genus. From https://www.ocean.udel.edu/cms/thanson/TH-MicroRev.pdf Hanson R and Hanson T, 1996.].































References

Jun Tsubota, Bulat Ts. Eshinimaev, Valentina N. Khmelenina and Yuri A. Trotsenko. 2005. "Methylothermus thermalis gen. nov., sp. nov.,a novel moderately thermophilic obligate methanotroph from a hot spring in Japan". International Journal of Systematic and Evolutionary Microbiology. 55, 1877–1884.

Hanson R and Hanson T. 1996. "Methanotrophic Bacteria". American Society for Microbiology, Vol. 60, No. 2.

Author

Page authored by Anthony Heidt and James Goodrow Jr., student of Prof. Jay Lennon at Michigan State University.