Pneumocystis carinii: Difference between revisions
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'''"Transcriptome of Pneumocystis carinii during Fulminate Infection: Carbohydrate Metabolism and the Concept of a Compatible Parasite"''' | '''"Transcriptome of Pneumocystis carinii during Fulminate Infection: Carbohydrate Metabolism and the Concept of a Compatible Parasite"''' | ||
ESTs were taken from the poly(A) tails of ''P. carinii'' messenger RNA to determine what types of genes were expressed during an infection. This strategy does not provide a complete picture of all the genes that may be expressed by ''P. carinii'' since not all genes are expressed at one time. The greater majority of the transcripts observed were part of metabolic processes and stress responses. Nutrient limitations on the organisms increase after they have significantly compromised the oxygen flow in the alveoli. The CON7 gene, which is associated with sporogenesis in fungi, was found to be expressed in abundance. This suggests that nutrient deprivation can cause ''P. carinii'' to enter the sexual reproductive stage of it's life cycle. Thus, thus the oxygen supplied by the host's biology is possibly the trigger for the organism to move on to the next stage in it's life cycle. Understanding what genes are being expressed during an infection is an important first step and determining drug targets unique to ''P. carinii'' in order to minimize collateral damage to the host. | ESTs were taken from the poly(A) tails of ''P. carinii'' messenger RNA to determine what types of genes were expressed during an infection. This strategy does not provide a complete picture of all the genes that may be expressed by ''P. carinii'' since not all genes are expressed at one time. The greater majority of the transcripts observed were part of metabolic processes and stress responses. Nutrient limitations on the organisms increase after they have significantly compromised the oxygen flow in the alveoli. The CON7 gene, which is associated with sporogenesis in fungi, was found to be expressed in abundance. This suggests that nutrient deprivation can cause ''P. carinii'' to enter the sexual reproductive stage of it's life cycle. Thus, thus the oxygen supplied by the host's biology is possibly the trigger for the organism to move on to the next stage in it's life cycle and the reason why it has not been seen outside of a host. Understanding what genes are being expressed during an infection is an important first step and determining drug targets unique to ''P. carinii'' in order to minimize collateral damage to the host. | ||
'''"Parameters Determining the Relative Efficacy of Hydroxy-naphthoquinone Inhibitors of the Cytochrome bc1 Complex"''' | |||
With the rise in drug resistances in ''P. carinii'' it is very important to find ways to regain antimicrobial supremacy with the development of new drugs. Atovaquone®, a hydroxy-naphthoquinone is currently being used to competitively inhibit the cytochrome ''bc1'' complex that binds the ubiquinol oxidation site between cytochrome ''b'' and the iron-sulfur protein. Yeast and bovine versions of the complex were used to test modified hydroxy-naphthoquinones against the mutated cytochrome ''b'' gene in ''P. carinii''. A steroisomer was developed and proved successful against the mutant. However, it could be quickly broken down by the human body. Despite the shortcomings experienced the new steroisomer represents a new lead to be used in the fight against resistance in ''P. carinii''. | |||
==References== | ==References== | ||
Revision as of 08:00, 29 August 2007
A Microbial Biorealm page on the genus Pneumocystis carinii
Classification
Higher order taxa
Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Pneumocystidomycetes; Pneumocystidaceae; Pneumocystis
Species
Pneumocystis carinii
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NCBI: Taxonomy |
Description and significance
From the genus Pneumocystis, Pneumocystis carinii, is a fungal opportunistic pathogen which causes the disease Pneumocystis pneumonia (PCP) in immunocompromised mammalian hosts, specifically rats. Pneumocystis carinii was originally thought to be a developmental stage in the life cycle of Trypanosoma when it was observed in 1909. In 1912 at the Pasteur Institute it was found to be a different and new organism. However, in 1988, the organism was reclassified as a member of the fungal kingdom after its small ribosomal RNA subunit had been sequenced. The full genome has yet to be sequenced, but progress is currently underway, see Genome structure (1). The initial classification of Pneumocystis carinii as a protozoa was partly due to the life-cycle of the organism.
Although Pneumocystis carinii has yet to be grown in pure culture, aspects of its life-cycle have been observed within infected mammalian lung tissue (see cell structure). During its known life-cycle, P. carinii can be found as trophic and cystic forms (2).
The HIV pandemic elevated interest in Pneumocystis carinii since it is the pathogen which caused Pneumocystis pneumonia and consequently a major cause of mortality in patients diagnosed with AIDS (see pathology). Despite successes against P. carinii with Trimethoprim with sulfamethoxazole there are signs that drug target sights may be mutating (1).
It should be noted that both Pneumocystis carinii and Pneumocystis jirovecii currently refer to the same organism in medical literature with regards to PCP. P. jirovecii is the organism isolated from humans while P. carinii is found in rats(3). The two organisms cause PCP in their respective hosts.
Genome structure
Sequencing of the Pneumocystis carinii is currently in progress and knowledge about the gnome has been advanced by groups such as the Pneumocystis Genome Project and the Wellcome Trust Sanger Institute Pneumocystis carnii telomeric clone sequencing project in conjunction with the University of Cincinnati, the University of Georgia, and the University of Kentucky. Information from these programs has not been made directly available to the public at this point in time.
The P. carinii genome is contained by 15 chromosomes which are from 300 kb to 700 kb in size. On these 15 chromosomes there are approximately 8 million base pairs of DNA. One feature of the P. carinii genome is that the organism has a high concentration of adenine and thymine bases (65% of the total base pairs). The genome has introns contain 38 to 424 base pairs and there have been up to nine introns per gene. The yeast Schizosaccharomyces pombe has been used as a homologue for the expressed sequenced tags (ESTs) found within the P. carinii genome (4). Completion of genomic sequence will prove invaluable in unlocking the biology of Pneumocystis carinii beyond what has been determined through the study of infected mammalian lung tissue.
Cell structure and metabolism
Like other fungi, Pneumocystis carinii has chitin in its cell wall. The cyst form also contains β-1,3-glucan. This strucutal protein may help elicit an inflammatory response from the host and, subsequently, may be one of the factors contributing damage to the alveolar epithelium. P. carinii uses mannose carbohydrates to adhere to the host (1). These unique surface proteins may provide future drug targets.
Pneumocystis carinii exists within the host in two forms: the trophic and the cyst. The trophozoites average a diameter of 1-4µm while the cysts are 8-10µm. The trophic forms of P. carinii may reproduce asexually via binary fission while cysts are able to reproduce sexually after trophozoites conjugate (2). The cysts develop from in three stages with increasing amounts of intracystic nuclei in each stage. The early cyst stage contains two intracystic nuclei while the intermediate stage has 4 and the late precysts stage holds 8. The mature cysts then release the trophic form of P. carinii as they burst. These observations on the life cycle have been made without the organism growing in a pure culture and contradict the life cycles of other ascomyceteous fungi (1). The life cycle is similar to those observed in protozoa and led to the initial classification of P. carinii as a protozoon.
The metabolism of lipids by Pneumocystis carinii has been unlocked by the use of radiolabeled precursors in purified samples of the organism. P. carinii is capable of incorporating ethanolamine and choline precursors into phospholipid head groups. Additionally, it was found that the organism is capable of metabolizing PE to PC by the methylation of the glycerophospholipid head group. Experiments using radiolabeled serine and inositol have established that P. carinii might be able to convert PS to PI.
Ecology
P. carinii is found in the lung tissue of immunocompetent and immunocompromised hosts. The organism adheres specifically to the epithelium of the alveoli within the lug. Only hosts with compromised immune systems are at risk of developing PCP from P. carinii while a normal host will mount an adequate immune response to contain P. carinii.
Fragments of DNA identical to DNA sequences found in P. carinii have been found in ambient air(5) and pond water (6). Even though P. carinii nucleic acids have been found outside of the host environment, there is no definitive evidence to support an ecological niche for Pneumocystis carinii outside of a host environment.
Pathology
When Pneumocystis carinii enters the lungs of a host with a normal immune system the pathogenic fungi does not cause Pneumocystis pneumonia. The onset of PCP is the result an infection within a host that has a compromised immune system because the organism is able to proliferate and which results in pathogenesis. P. carinii fails to cause PCP in a host with a normal immune system because it is an opportunistic pathogen which is kept in check by a host with a fully functional immune response. Symptoms of PCP include: shortness of breath, non-productive cough, fever, malaise, and chest pain. An infection is fatal, if left untreated, because the trophic form of P. carinii binds to the type I pneumocytes and prevents gas to be exchanged between capillaries and the alveoli. This blockage leads to poor oxygenation and hypoxemia followed by death (2). Treatments include the use of trimethoprim with sulfamethoxazole or pentamidine.
Current Research
"Transcriptome of Pneumocystis carinii during Fulminate Infection: Carbohydrate Metabolism and the Concept of a Compatible Parasite"
ESTs were taken from the poly(A) tails of P. carinii messenger RNA to determine what types of genes were expressed during an infection. This strategy does not provide a complete picture of all the genes that may be expressed by P. carinii since not all genes are expressed at one time. The greater majority of the transcripts observed were part of metabolic processes and stress responses. Nutrient limitations on the organisms increase after they have significantly compromised the oxygen flow in the alveoli. The CON7 gene, which is associated with sporogenesis in fungi, was found to be expressed in abundance. This suggests that nutrient deprivation can cause P. carinii to enter the sexual reproductive stage of it's life cycle. Thus, thus the oxygen supplied by the host's biology is possibly the trigger for the organism to move on to the next stage in it's life cycle and the reason why it has not been seen outside of a host. Understanding what genes are being expressed during an infection is an important first step and determining drug targets unique to P. carinii in order to minimize collateral damage to the host.
"Parameters Determining the Relative Efficacy of Hydroxy-naphthoquinone Inhibitors of the Cytochrome bc1 Complex"
With the rise in drug resistances in P. carinii it is very important to find ways to regain antimicrobial supremacy with the development of new drugs. Atovaquone®, a hydroxy-naphthoquinone is currently being used to competitively inhibit the cytochrome bc1 complex that binds the ubiquinol oxidation site between cytochrome b and the iron-sulfur protein. Yeast and bovine versions of the complex were used to test modified hydroxy-naphthoquinones against the mutated cytochrome b gene in P. carinii. A steroisomer was developed and proved successful against the mutant. However, it could be quickly broken down by the human body. Despite the shortcomings experienced the new steroisomer represents a new lead to be used in the fight against resistance in P. carinii.
References
Edited by Daniel Spisak of Rachel Larsen
