Tomaio L Pseudomonas fluorescens: Difference between revisions
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===Species=== | ===Species=== | ||
Genus: Pseudomonas | Genus: Pseudomonas | ||
Species: | Species: Pseudomonas Fluorescens | ||
Pseudomonas | |||
==Habitat Information == | ==Habitat Information == | ||
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==Description and Significance== | ==Description and Significance== | ||
'''Colonial Morphology:''' | '''Colonial Morphology:''' | ||
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Margin:Smooth | Margin:Smooth | ||
'''Cell Morphology:''' | |||
| Line 65: | Line 65: | ||
* Aerobic | * Aerobic | ||
* Motile | |||
* Nonspore-forming | * Nonspore-forming | ||
'''Test Results''' | |||
* Negative catalase test (this result is questionable) | |||
* Negative oxidase test (this result is questionable) | |||
* Positive | * Positive EMB for G- growth | ||
* Positive EMB for lactose fermentation | |||
* Positive MAC for G- growth | |||
* Positive HE for G- growth | |||
* Positive arganine decarboxylation test | |||
* Positive Blood Agar test | |||
* Positive Urea test | |||
* VP test was questionable | |||
'''Antimicrobial activity :''' | |||
Most Psuedomonas species are penicillin resistant, so other choices for treatment might include Ciprofloxacin, ticarcillin, imipenem or piperacillin. This resistance is due to their cell walls which contain prions. [1] | |||
'''My Results''' | |||
Ceftazidine proved very effective | |||
Clove and tea tree oil very effective | |||
==Genome Structure== | ==Genome Structure== | ||
Pseudomonas fluorescens Strain DH-27 16S Ribosomal | |||
Long Circular chromosome | |||
Max score: 1291 | |||
Total score: 1291 | |||
Query Cover: 100% | |||
E Value: 0.0 | |||
Ident: 99% | |||
Accession: KF646699.1 | |||
==Cell Structure, Metabolism and Life Cycle== | ==Cell Structure, Metabolism and Life Cycle== | ||
P. fluorescens is an anerobe and uses sideophores when its iron levels are low. when the sidephores are being activated this is what gives off the fluorescent color. [3] | |||
==Physiology and Pathogenesis== | ==Physiology and Pathogenesis== | ||
Pseudomonas fluorescens may be useful in protecting plants from fungus and nemotade attacks.[2] P. fluorescens can be found in soil, water and plant surfaces. It is well suited to survive in mineral content soil and is being studied for its use for biocontrol in agriculture. Monsanto has detected the natural pesticide abilities and has even spliced P. fluorescens with Ecoli and added another gene to create a constant glow from the modified bugs to help track and prove that these bugs would not spread uncontrollable if used as a pesticide in the fields. [5] | |||
P. fluorescens demonstrates hemolytic activity and, as a result, has been known to infect blood transfusions.[2] | |||
It is also used in milk to make yogurt.[2] | |||
'''Disease:''' | |||
P. fluorescens can be found infecteing those who are immunocompromised including cancer patients.[2] | |||
'''Medical Properties:''' | |||
Psuedomonas fluorescens has hemolytic properties and can infect blood transfusion. It can also be used as a strater for yogurts. | |||
P. fluorescens can be used to make the antibiotic Mupirocin which can be used to treati MRSA, skin, ear, and eye disorders.[2] | |||
==References== | ==References== | ||
1. http://en.m.wikipedia.org/wiki/Pseudomonas | |||
2. http://en.wikipedia.org/wiki/Pseudomonas_fluorescens | |||
3. https://microbewiki.kenyon.edu/index.php/Pseudomonas_fluorescens | |||
http:// | 4. http://genome.jgi-psf.org/psefl/psefl.home.html | ||
http:// | 5. http://www.nytimes.com/1986/09/18/business/technology-putting-a-tag-on-microbes.html | ||
==Author== | ==Author== | ||
Latest revision as of 18:05, 8 May 2015
Classification
Genus species Pseudomonas lini Scientific classification Kingdom: Bacteria
Phylum: Proteobacteria
Class: Gamma Proteobacteria
Order: Pseudomonadales
Family: Pseudomonadaceae
Species
Genus: Pseudomonas
Species: Pseudomonas Fluorescens
Habitat Information
Date of collection: 1/27/15
Location: 6636 W. William cannon Dr. Austin, TX 78735
Lat/:on: 30.27N 97.74W (Elev. 508ft)
Air Temperature: 80 degrees F
Humidity: 23%
24 hr rainfall: 0
Solar Radiation: 12.84
Description and Significance
Colonial Morphology:
Color:white
Elevation:Convex
Margin:Smooth
Cell Morphology:
Members of the genus display these defining characteristics:
* Rod-shaped
* Gram-negative
*One or more polar flagella, providing motility
* Aerobic
* Motile
* Nonspore-forming
Test Results
* Negative catalase test (this result is questionable) * Negative oxidase test (this result is questionable)
* Positive EMB for G- growth * Positive EMB for lactose fermentation * Positive MAC for G- growth * Positive HE for G- growth * Positive arganine decarboxylation test * Positive Blood Agar test * Positive Urea test * VP test was questionable
Antimicrobial activity :
Most Psuedomonas species are penicillin resistant, so other choices for treatment might include Ciprofloxacin, ticarcillin, imipenem or piperacillin. This resistance is due to their cell walls which contain prions. [1]
My Results Ceftazidine proved very effective Clove and tea tree oil very effective
Genome Structure
Pseudomonas fluorescens Strain DH-27 16S Ribosomal
Long Circular chromosome
Max score: 1291 Total score: 1291 Query Cover: 100% E Value: 0.0 Ident: 99% Accession: KF646699.1
Cell Structure, Metabolism and Life Cycle
P. fluorescens is an anerobe and uses sideophores when its iron levels are low. when the sidephores are being activated this is what gives off the fluorescent color. [3]
Physiology and Pathogenesis
Pseudomonas fluorescens may be useful in protecting plants from fungus and nemotade attacks.[2] P. fluorescens can be found in soil, water and plant surfaces. It is well suited to survive in mineral content soil and is being studied for its use for biocontrol in agriculture. Monsanto has detected the natural pesticide abilities and has even spliced P. fluorescens with Ecoli and added another gene to create a constant glow from the modified bugs to help track and prove that these bugs would not spread uncontrollable if used as a pesticide in the fields. [5]
P. fluorescens demonstrates hemolytic activity and, as a result, has been known to infect blood transfusions.[2]
It is also used in milk to make yogurt.[2]
Disease:
P. fluorescens can be found infecteing those who are immunocompromised including cancer patients.[2]
Medical Properties:
Psuedomonas fluorescens has hemolytic properties and can infect blood transfusion. It can also be used as a strater for yogurts. P. fluorescens can be used to make the antibiotic Mupirocin which can be used to treati MRSA, skin, ear, and eye disorders.[2]
References
1. http://en.m.wikipedia.org/wiki/Pseudomonas
2. http://en.wikipedia.org/wiki/Pseudomonas_fluorescens
3. https://microbewiki.kenyon.edu/index.php/Pseudomonas_fluorescens
4. http://genome.jgi-psf.org/psefl/psefl.home.html
5. http://www.nytimes.com/1986/09/18/business/technology-putting-a-tag-on-microbes.html
Author
Page authored by Louisa Tomaio, student of Prof. Kristine Hollingsworth at Austin Community College.
