Tomaio L Pseudomonas fluorescens: Difference between revisions

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===Species===
===Species===


''Genus species''


Genus: Pseudomonas
Genus: Pseudomonas


Species: P. lini
Species: Pseudomonas Fluorescens
Binomial name
Pseudomonas lini


==Habitat Information ==
==Habitat Information ==
Line 69: Line 65:


   * Aerobic
   * Aerobic
  *  Motile


   * Nonspore-forming
   * Nonspore-forming


  * Positive catalase test
'''Test Results'''
  * Negative catalase test (this result is questionable)
  * Negative oxidase test (this result is questionable)
 
  *  Positive EMB for G- growth
  *  Positive EMB for lactose fermentation
  *  Positive MAC for G- growth
  *  Positive HE for G- growth
  *  Positive arganine decarboxylation test
  *  Positive Blood Agar test
  *  Positive Urea test
  *  VP test was questionable
 
 
'''Antimicrobial activity :'''
 
Most Psuedomonas species are penicillin resistant, so other choices for treatment might include Ciprofloxacin, ticarcillin, imipenem or piperacillin.  This resistance is due to their cell walls which contain prions. [1]


  *  Positive oxidase test
'''My Results'''
Ceftazidine proved very effective
Clove and tea tree oil very effective


==Genome Structure==
Pseudomonas fluorescens Strain DH-27 16S Ribosomal


Other characteristics that tend to be associated with Pseudomonas species (with some exceptions) include secretion of pyoverdine, a fluorescent yellow-green siderophore[12] under iron-limiting conditions. Certain Pseudomonas species may also produce additional types of siderophore, such as pyocyanin by Pseudomonas aeruginosa[13] and thioquinolobactin by Pseudomonas fluorescens,.[14] Pseudomonas species also typically give a positive result to the oxidase test, the absence of gas formation from glucose, glucose is oxidised in oxidation/fermentation test using Hugh and Leifson O/F test, beta hemolytic (on blood agar), indole negative, methyl red negative, Voges–Proskauer test negative, and citrate positive.
Long Circular chromosome


Pseudomonas may be the most common nucleator of ice crystals in clouds, thereby being of utmost importance to the formation of snow and rain around the world.
Max score: 1291
Total score: 1291
Query Cover: 100%
E Value: 0.0
Ident: 99%
Accession: KF646699.1


==Cell Structure, Metabolism and Life Cycle==


P. fluorescens is an anerobe and uses sideophores when its iron levels are low.  when the sidephores are being activated this is what gives off the fluorescent color. [3]


'''Antimicrobial activity :'''
==Physiology and Pathogenesis==
Being Gram-negative bacteria, most Pseudomonas spp. are naturally resistant to penicillin and the majority of related beta-lactam antibiotics, but a number are sensitive to piperacillin, imipenem, ticarcillin, or ciprofloxacin.[17] Aminoglycosides such as tobramycin, gentamicin, and amikacin are other choices for therapy.


This ability to thrive in harsh conditions is a result of their hardy cell walls that contain porins. Their resistance to most antibiotics is attributed to efflux pumps, which pump out some antibiotics before they are able to act.
Pseudomonas fluorescens may be useful in protecting plants from fungus and nemotade attacks.[2]  P. fluorescens can be found in soil, water and plant surfaces.  It is well suited to survive in mineral content soil and is being studied for its use for biocontrol in agriculture.  Monsanto has detected the natural pesticide abilities and has even spliced P. fluorescens with Ecoli and added another gene to create a constant glow from the modified bugs to help track and prove that these bugs would not spread uncontrollable if used as a pesticide in the fields. [5]


Pseudomonas aeruginosa is increasingly recognized as an emerging opportunistic pathogen of clinical relevance. One of its most worrying characteristics is its low antibiotic susceptibility.[18] This low susceptibility is attributable to a concerted action of multidrug efflux pumps with chromosomally encoded antibiotic resistance genes (e.g., mexAB-oprM, mexXY, etc.,[19]) and the low permeability of the bacterial cellular envelopes. Besides intrinsic resistance, P. aeruginosa easily develops acquired resistance either by mutation in chromosomally encoded genes or by the horizontal gene transfer of antibiotic resistance determinants. Development of multidrug resistance by P. aeruginosa isolates requires several different genetic events that include acquisition of different mutations and/or horizontal transfer of antibiotic resistance genes. Hypermutation favours the selection of mutation-driven antibiotic resistance in P. aeruginosa strains producing chronic infections, whereas the clustering of several different antibiotic resistance genes in integrons favours the concerted acquisition of antibiotic resistance determinants. Some recent studies have shown phenotypic resistance associated to biofilm formation or to the emergence of small-colony-variants may be important in the response of P. aeruginosa populations to antibiotic treatment
P. fluorescens demonstrates hemolytic activity and, as a result, has been known to infect blood transfusions.[2]


'''Genome sequencing'''
It is also used in milk to make yogurt.[2]


Pseudomonas fluorescens Strain DH-27 165 Ribosomal


==Genome Structure==
'''Disease:'''
Describe the size and content of the genome.  How many chromosomes?  Circular or linear?  Other interesting features?  What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.


P. fluorescens can be found infecteing those who are immunocompromised including cancer patients.[2]


==Cell Structure, Metabolism and Life Cycle==
Interesting features of cell structure; how it gains energy; what important molecules it produces.


'''Medical Properties:'''


==Physiology and Pathogenesis==
Psuedomonas fluorescens has hemolytic properties and can infect blood transfusion.  It can also be used as a strater for yogurts.
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).<br>
P. fluorescens can be used to make the antibiotic Mupirocin which can be used to treati MRSA, skin, ear, and eye disorders.[2]
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.<br><br>


==References==
==References==


1. http://en.m.wikipedia.org/wiki/Pseudomonas


http://en.wikipedia.org/wiki/Pseudomonas_lini
2. http://en.wikipedia.org/wiki/Pseudomonas_fluorescens


http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=163011
3. https://microbewiki.kenyon.edu/index.php/Pseudomonas_fluorescens


http://gcm.wfcc.info/speciesPage.jsp?strain_name=Pseudomonas%20lini
4. http://genome.jgi-psf.org/psefl/psefl.home.html


http://en.wikipedia.org/wiki/Pseudomonas_fluorescens
5. http://www.nytimes.com/1986/09/18/business/technology-putting-a-tag-on-microbes.html


==Author==
==Author==

Latest revision as of 18:05, 8 May 2015

This student page has not been curated.

Classification

Genus species Pseudomonas lini Scientific classification Kingdom: Bacteria

Phylum: Proteobacteria

Class: Gamma Proteobacteria

Order: Pseudomonadales

Family: Pseudomonadaceae


Species

Genus: Pseudomonas

Species: Pseudomonas Fluorescens

Habitat Information

Date of collection: 1/27/15

Location: 6636 W. William cannon Dr. Austin, TX 78735

Lat/:on: 30.27N 97.74W (Elev. 508ft)

Air Temperature: 80 degrees F

Humidity: 23%

24 hr rainfall: 0

Solar Radiation: 12.84

Description and Significance

Colonial Morphology:

Color:white

Elevation:Convex

Margin:Smooth


Cell Morphology:


Members of the genus display these defining characteristics: 

  * Rod-shaped

  * Gram-negative

   *One or more polar flagella, providing motility

  * Aerobic

 *  Motile

  * Nonspore-forming

Test Results 

 * Negative catalase test (this result is questionable)
 * Negative oxidase test (this result is questionable)

 *  Positive EMB for G- growth
 *  Positive EMB for lactose fermentation
 *  Positive MAC for G- growth
 *  Positive HE for G- growth
 *  Positive arganine decarboxylation test
 *  Positive Blood Agar test
 *  Positive Urea test
 *  VP test was questionable


Antimicrobial activity :

Most Psuedomonas species are penicillin resistant, so other choices for treatment might include Ciprofloxacin, ticarcillin, imipenem or piperacillin. This resistance is due to their cell walls which contain prions. [1]

My Results Ceftazidine proved very effective Clove and tea tree oil very effective

Genome Structure

Pseudomonas fluorescens Strain DH-27 16S Ribosomal

Long Circular chromosome

Max score: 1291 Total score: 1291 Query Cover: 100% E Value: 0.0 Ident: 99% Accession: KF646699.1

Cell Structure, Metabolism and Life Cycle

P. fluorescens is an anerobe and uses sideophores when its iron levels are low. when the sidephores are being activated this is what gives off the fluorescent color. [3]

Physiology and Pathogenesis

Pseudomonas fluorescens may be useful in protecting plants from fungus and nemotade attacks.[2] P. fluorescens can be found in soil, water and plant surfaces. It is well suited to survive in mineral content soil and is being studied for its use for biocontrol in agriculture. Monsanto has detected the natural pesticide abilities and has even spliced P. fluorescens with Ecoli and added another gene to create a constant glow from the modified bugs to help track and prove that these bugs would not spread uncontrollable if used as a pesticide in the fields. [5]

P. fluorescens demonstrates hemolytic activity and, as a result, has been known to infect blood transfusions.[2]

It is also used in milk to make yogurt.[2]


Disease:

P. fluorescens can be found infecteing those who are immunocompromised including cancer patients.[2]


Medical Properties:

Psuedomonas fluorescens has hemolytic properties and can infect blood transfusion. It can also be used as a strater for yogurts. P. fluorescens can be used to make the antibiotic Mupirocin which can be used to treati MRSA, skin, ear, and eye disorders.[2]

References

1. http://en.m.wikipedia.org/wiki/Pseudomonas

2. http://en.wikipedia.org/wiki/Pseudomonas_fluorescens

3. https://microbewiki.kenyon.edu/index.php/Pseudomonas_fluorescens

4. http://genome.jgi-psf.org/psefl/psefl.home.html

5. http://www.nytimes.com/1986/09/18/business/technology-putting-a-tag-on-microbes.html

Author

Page authored by Louisa Tomaio, student of Prof. Kristine Hollingsworth at Austin Community College.

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