Tomaio L Pseudomonas fluorescens

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Classification

Genus species Pseudomonas lini Scientific classification Kingdom: Bacteria

Phylum: Proteobacteria

Class: Gamma Proteobacteria

Order: Pseudomonadales

Family: Pseudomonadaceae

Species

Genus: Pseudomonas

Species: Pseudomonas Fluorescens

Habitat Information

Date of collection: 1/27/15

Location: 6636 W. William cannon Dr. Austin, TX 78735

Lat/:on: 30.27N 97.74W (Elev. 508ft)

Air Temperature: 80 degrees F

Humidity: 23%

24 hr rainfall: 0

Solar Radiation: 12.84

Description and Significance

Colonial Morphology:

Color:white

Elevation:Convex

Margin:Smooth

Cell Morphology:

Members of the genus display these defining characteristics:

  * Rod-shaped

  * Gram-negative

   *One or more polar flagella, providing motility

  * Aerobic

  * Nonspore-forming

Test Results

 * Negative catalase test (this result is questionable)
 * Negative oxidase test (this result is questionable)

 *  Positive EMB for G- growth
 *  Positive EMB for lactose fermentation
 *  Positive MAC for G- growth
 *  Positive HE for G- growth
 *  Positive arganine decarboxylation test
 *  Positive Blood Agar test
 *  Positive Urea test
 *  VP test was questionable

Antimicrobial activity :

Most Psuedomonas species are penicillin resistant, so other choices for treatment might include Ciprofloxacin, ticarcillin, imipenem or piperacillin. This resistance is due to their cell walls which contain prions. [1]

My Results Ceftazidine proved very effective Clove and tea tree oil very effective

Genome Structure

Pseudomonas fluorescens Strain DH-27 16S Ribosomal

Long Circular chromosome

Max score: 1291 Total score: 1291 Query Cover: 100% E Value: 0.0 Ident: 99% Accession: KF646699.1

Cell Structure, Metabolism and Life Cycle

Pseudomonas fluorescens produces phloroglucinol, phloroglucinol carboxylic acid and diacetylphloroglucinol. Biodegradation capacities

4-Hydroxyacetophenone monooxygenase is an enzyme found in P. fluorescens that transform piceol, NADPH, H+ and O2 into 4-hydroxyphenyl acetate, NADP+ and H2O.

Physiology and Pathogenesis

Pseudomonas fluorescens may be useful in protecting plants from fungus and nemotade attacks.

By culturing Pseudomonas fluorescens, Mupirocin (an antibiotic) can be produced, which has been found to be useful in treating skin, ear, and eye disorders.[19] Mupirocin free acid and its salts and esters are agents currently used in creams, ointments, and sprays as a treatment of Methicillin-resistant Staphylococcus aureus (MRSA) infection.

P. fluorescens demonstrates hemolytic activity and, as a result, has been known to infect blood transfusions.

It is also used in milk to make yogurt.

Disease:

P. fluorescens is an unusual cause of disease in humans, and usually affects patients with compromised immune systems (e.g., patients on cancer treatment). From 2004 to 2006, there was an outbreak of P. fluorescens in the United States, involving 80 patients in six states. The source of the infection was contaminated heparinized saline flushes being used with cancer patients.

Medical Properties:

Psuedomonas fluorescens has hemolytic properties and can infect blood transfusion. It can also be used as a strater for yogurts. P. fluorescens can be used to make the antibiotic Mupirocin which can be used to treati MRSA, skin, ear, and eye disorders.

References

1. http://en.m.wikipedia.org/wiki/Pseudomonas

2. http://en.wikipedia.org/wiki/Pseudomonas_fluorescens

Author

Page authored by Louisa Tomaio, student of Prof. Kristine Hollingsworth at Austin Community College.