Tomaio L Pseudomonas fluorescens

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Classification

Genus species Pseudomonas lini Scientific classification Kingdom: Bacteria

Phylum: Proteobacteria

Class: Gamma Proteobacteria

Order: Pseudomonadales

Family: Pseudomonadaceae

Species

Genus: Pseudomonas

Species: Pseudomonas Fluorescens

Habitat Information

Date of collection: 1/27/15

Location: 6636 W. William cannon Dr. Austin, TX 78735

Lat/:on: 30.27N 97.74W (Elev. 508ft)

Air Temperature: 80 degrees F

Humidity: 23%

24 hr rainfall: 0

Solar Radiation: 12.84

Description and Significance

Colonial Morphology:

Color:white

Elevation:Convex

Margin:Smooth

Cell Morphology:

Members of the genus display these defining characteristics:

  * Rod-shaped

  * Gram-negative

   *One or more polar flagella, providing motility

  * Aerobic

  * Nonspore-forming

Test Results

 * Negative catalase test (this result is questionable)
 * Negative oxidase test (this result is questionable)

 *  Positive EMB for G- growth
 *  Positive EMB for lactose fermentation
 *  Positive MAC for G- growth
 *  Positive HE for G- growth
 *  Positive arganine decarboxylation test
 *  Positive Blood Agar test
 *  Positive Urea test
 *  VP test was questionable

Antimicrobial activity :

Most Psuedomonas species are penicillin resistant, so other choices for treatment might include Ciprofloxacin, ticarcillin, imipenem or piperacillin. This resistance is due to their cell walls which contain prions. [1]

My Results Ceftazidine proved very effective Clove and tea tree oil very effective

Genome Structure

Pseudomonas fluorescens Strain DH-27 16S Ribosomal

Long Circular chromosome

Max score: 1291 Total score: 1291 Query Cover: 100% E Value: 0.0 Ident: 99% Accession: KF646699.1

Cell Structure, Metabolism and Life Cycle

Pseudomonas fluorescens produces phloroglucinol, phloroglucinol carboxylic acid and diacetylphloroglucinol. Biodegradation capacities

4-Hydroxyacetophenone monooxygenase is an enzyme found in P. fluorescens that transform piceol, NADPH, H+ and O2 into 4-hydroxyphenyl acetate, NADP+ and H2O.

Physiology and Pathogenesis

Pseudomonas fluorescens may be useful in protecting plants from fungus and nemotade attacks.[2]

P. fluorescens demonstrates hemolytic activity and, as a result, has been known to infect blood transfusions.[2]

It is also used in milk to make yogurt.[2]

Disease:

P. fluorescens can be found infecteing those who are immunocompromised including cancer patients.[2]

Medical Properties:

Psuedomonas fluorescens has hemolytic properties and can infect blood transfusion. It can also be used as a strater for yogurts. P. fluorescens can be used to make the antibiotic Mupirocin which can be used to treati MRSA, skin, ear, and eye disorders.[2]

References

1. http://en.m.wikipedia.org/wiki/Pseudomonas

2. http://en.wikipedia.org/wiki/Pseudomonas_fluorescens

Author

Page authored by Louisa Tomaio, student of Prof. Kristine Hollingsworth at Austin Community College.