Tomaio L Pseudomonas fluorescens

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Classification

Genus species Pseudomonas lini Scientific classification Kingdom: Bacteria

Phylum: Proteobacteria

Class: Gamma Proteobacteria

Order: Pseudomonadales

Family: Pseudomonadaceae

Species

Genus species

Genus: Pseudomonas

Species: P. lini Binomial name Pseudomonas lini

Habitat Information

Date of collection: 1/27/15

Location: 6636 W. William cannon Dr. Austin, TX 78735

Lat/:on: 30.27N 97.74W (Elev. 508ft)

Air Temperature: 80 degrees F

Humidity: 23%

24 hr rainfall: 0

Solar Radiation: 12.84

Description and Significance

Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.

Colonial Morphology:

Color:white

Elevation:Convex

Margin:Smooth

Cell Morphology:

Members of the genus display these defining characteristics:

  * Rod-shaped

  * Gram-negative

   *One or more polar flagella, providing motility

  * Aerobic

  * Nonspore-forming

  * Positive catalase test

 *  Positive oxidase test

Other characteristics that tend to be associated with Pseudomonas species (with some exceptions) include secretion of pyoverdine, a fluorescent yellow-green siderophore[12] under iron-limiting conditions. Certain Pseudomonas species may also produce additional types of siderophore, such as pyocyanin by Pseudomonas aeruginosa[13] and thioquinolobactin by Pseudomonas fluorescens,.[14] Pseudomonas species also typically give a positive result to the oxidase test, the absence of gas formation from glucose, glucose is oxidised in oxidation/fermentation test using Hugh and Leifson O/F test, beta hemolytic (on blood agar), indole negative, methyl red negative, Voges–Proskauer test negative, and citrate positive.

Pseudomonas may be the most common nucleator of ice crystals in clouds, thereby being of utmost importance to the formation of snow and rain around the world.

Antimicrobial activity :

Genome Structure

Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.

Cell Structure, Metabolism and Life Cycle

Interesting features of cell structure; how it gains energy; what important molecules it produces.

Physiology and Pathogenesis

Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

References

http://en.wikipedia.org/wiki/Pseudomonas_lini

http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=163011

http://gcm.wfcc.info/speciesPage.jsp?strain_name=Pseudomonas%20lini

Author

Page authored by Louisa Tomaio, student of Prof. Kristine Hollingsworth at Austin Community College.