User:KosinskiJ
A Microbial Biorealm page on the genus KosinskiJ
Classification
Higher order taxa
Bacteria; Spirochaetes; Spirochaetales; Leptospiraceae; Leptospira; Leptospira interrogans [1].
Species
NCBI: Taxonomy [1] |
Leptospira interrogans
Description and significance
The Leptospira genus comprises a heterogeneous group of pathogenic and saprophytic species belonging to the phylum Spirochaetes [2]. Leptospires are thin, helically coiled, motile, Gram negative, obligate aerobic bacteria, classified into hundreds of pathogenic serovars [2]. They live optimally at temperatures between 28-30°C [3].
Genome structure
L. interrogans genome is circular and contains two chromosomes, a large and small, both containing 4,277,185 and 350,181 base pairs respectively, roughly 4,768 genes [4].Their genomes consist of 35% G-C content. The large chromosome contains Ribosomal RNA genes: two 23S, two 16S and one 5S genes [4]. They contain 37 genes encoding transfer RNAs. The low number of tRNA and rRNA genes may be attributed to the fastidious growth of the L. interrogans. [5].
Cell structure and metabolism
Given that L. interrogans is a Gram negative microorganism, the outer membrane consists of lipopolysaccharide proteins (LPS) [6]. Spirochetal outer membrane proteins, mostly porins, are predicted to span the lipid bilayer in multiple amphipathic, membrane-spanning beta sheets arranged in a barrel [6]. The outer face of the beta-sheets is hydrophobic and interacts with the lipid bilayer, while the inner face is hydrophilic and interacts with the aqueous pore of the protein [6].
L. interrogans are found to be obligate aerobes and undergo oxidative respiration for growth and survival [2]. They are physiologically chemoheterotrophic [5].
Ecology
The habitat in which this organism resides is typically in tropical and subtropical regions after heavy rainfall and dispersal of leptospires in contaminated water [6].
Pathology
This organism is the causative agent of leptospirosis, a tropical zoonosis transmitted by direct contact with the urine of infected animals via contaminated soil or fresh water [2]. These microorganisms have adapted for specific mammalian reservoir hosts, allowing them to harbor the hosts renal tubules and shed the Leptospira in their urine [4]. The range of carriers includes mammals, birds amphibians, reptiles and humans, who are rarely chronic carriers but can be accidental host [3]. For humans, fever, chills, headache, and severe myalgias characterize the early phase of the disease. In more severe cases, jaundice, renal, failure, and/or pulmonary hemorrhaging [6]. Progression to multi-organ system complications occurs in 5 to 15% of cases, with mortality rates of 5 to 40% [4]. Leptospirosis has recently been recognized as a ‘re-emerging infectious disease’ among humans and animals and has the potential to become even more prevalent with anticipated global warming [3].
Current Research and or Application to Biotechnology
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References
Edited by student of Dr. Lynn M Bedard, DePauw University http://www.depauw.edu