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<b><i>Ralstonia paucula</i></b>
1 Classification  
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==Classification==
1.1 Higher order taxa
 
1.2 Species
 
2 Description and significance
 
3 16S Ribosomal RNA Gene Information
 
4 Cell structure and metabolism
 
5 Ecology
 
6 Pathology
 
7 Current Research
 
8 References
 
 
  
[edit] Classification
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===Higher order taxa===
[edit] Higher order taxa
 
Proteobacteria, Bacteria, Ralstoniaceae, Ralstonia,
 
 
RALSTONIA PAUCULA
 
  
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Domain; Phylum; Class; Order; family [Others may be used.  Use [http://www.ncbi.nlm.nih.gov/Taxonomy/ NCBI] link to find]
  
Genus species
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===Species===
  
[edit] Description and significance
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{|
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| height="10" bgcolor="#FFDF95" |
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'''NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Tree&id=2&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy]'''
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|}
  
Ralstonia paucula is a Gram-negative environmental bacterium.
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''<i>Ralstonia paucula</i>''
Isolated from pool water, groundwater, bottled mineral water, and many clinical specimens. Ralstonia paucula, despite its low pathogenicity, is now recognized as an opportunist pathogen that can generate serious infections, such as septicemia, peritonitis, abscesses, etc., especially in immunocompromised patients
 
  
[edit] 16S Ribosomal RNA Gene Information
+
==Description and significance==
 +
<i>Ralstonia paucula</i> is a Gram-negative environmental bacterium.
 +
Isolated from pool water, groundwater, bottled mineral water, and many clinical specimens. Ralstonia paucula, despite its low pathogenicity, is now recognized as an opportunist pathogen that can generate serious infections, such as septicemia, peritonitis, abscesses, etc., especially in immunocompromised patients.
 
   
 
   
 +
==16S Ribosomal RNA Gene Information==
 
In 1995, bacteria was confronted with five cases of nosocomial bacteremia, we found that pulsed-field gel electrophoresis could not distinguish between the isolates and that randomly amplified polymorphic DNA analysis was poorly discriminatory. In this study, we used PCR-ribotyping and PCR-restriction fragment length polymorphism analysis of the spacer 16S-23S ribosomal DNA (rDNA); both methods were unable to differentiate R. paucula isolates. Eighteen strains belonging to other Ralstonia species (one R. eutropha strain, six R. pickettii strains, three R. solanacearum strains, and eight R. gilardii strains) were also tested by PCR-ribotyping, which failed to distinguish between the four species. The 16S-23S rDNA intergenic spacer of R. paucula contains the tRNAIle and tRNAAla genes, which are identical to genes described for R. pickettii and R. solanacearum. [1]
 
In 1995, bacteria was confronted with five cases of nosocomial bacteremia, we found that pulsed-field gel electrophoresis could not distinguish between the isolates and that randomly amplified polymorphic DNA analysis was poorly discriminatory. In this study, we used PCR-ribotyping and PCR-restriction fragment length polymorphism analysis of the spacer 16S-23S ribosomal DNA (rDNA); both methods were unable to differentiate R. paucula isolates. Eighteen strains belonging to other Ralstonia species (one R. eutropha strain, six R. pickettii strains, three R. solanacearum strains, and eight R. gilardii strains) were also tested by PCR-ribotyping, which failed to distinguish between the four species. The 16S-23S rDNA intergenic spacer of R. paucula contains the tRNAIle and tRNAAla genes, which are identical to genes described for R. pickettii and R. solanacearum. [1]
  
[edit] Cell structure and metabolism
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==Cell structure and metabolism==
 +
Interesting features of cell structure; how it gains energy; what important molecules it produces.
  
  
 +
==Ecology==
 +
Habitat; symbiosis; contributions to the environment.
  
 +
==Pathology==
 +
<i>R. paucula</i> can cause clusters of nosocomial infections. Treatment of such infections was mainly based on the use of beta-lactams such as cefotaxime, ceftriaxone, piperacillin, and imipenem.[1]
 +
==Current Research==
  
[edit] Ecology
 
 
 
[edit] Pathology
 
R. paucula can cause clusters of nosocomial infections. Treatment of such infections was mainly based on the use of beta-lactams such as cefotaxime, ceftriaxone, piperacillin, and imipenem.[1]
 
 
[edit] Current Research
 
  
  
[edit] References
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==References==
 +
<sup>[1]</sup>[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC87738/D Moissenet, D., Bidet, P., Garbarg-Chenon, A. Arlet, G., and Vu-Thien1, H. "Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)". J Clin Microbiol. 2001 January; 39(1): 381–384.]  
  
1. Didier Moissenet, Philippe Bidet, Antoine Garbarg-Chenon, Guillaume Arlet, and Hoang Vu-Thien1 "Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)", Journal of Clinical Microbiology, January 2001, p. 381-384, Vol. 39, No. 1
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Edited by Karolina Wala, Alma Shebib students of [mailto:mglogow@luc.edu M Glogowski]at [http://www.luc.edu/biology/index.html Loyola University]
 
 
Edited by Karolina Wala, Alma Shebib, students of M Glogowskiat Loyola University http://www.ncbi.nlm.nih.gov/pmc/articles/PMC87738/  
 
  
Retrieved from "http://microbewiki.kenyon.edu/index.php/Genus_glogowski"
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<!-- Do not edit or remove this line-->[[Category:Pages edited by students of Mary Glogowski at Loyola University]]

Revision as of 20:01, 3 May 2010

Ralstonia paucula

Classification

Higher order taxa

Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]

Species

NCBI: Taxonomy

Ralstonia paucula

Description and significance

Ralstonia paucula is a Gram-negative environmental bacterium. Isolated from pool water, groundwater, bottled mineral water, and many clinical specimens. Ralstonia paucula, despite its low pathogenicity, is now recognized as an opportunist pathogen that can generate serious infections, such as septicemia, peritonitis, abscesses, etc., especially in immunocompromised patients.

16S Ribosomal RNA Gene Information

In 1995, bacteria was confronted with five cases of nosocomial bacteremia, we found that pulsed-field gel electrophoresis could not distinguish between the isolates and that randomly amplified polymorphic DNA analysis was poorly discriminatory. In this study, we used PCR-ribotyping and PCR-restriction fragment length polymorphism analysis of the spacer 16S-23S ribosomal DNA (rDNA); both methods were unable to differentiate R. paucula isolates. Eighteen strains belonging to other Ralstonia species (one R. eutropha strain, six R. pickettii strains, three R. solanacearum strains, and eight R. gilardii strains) were also tested by PCR-ribotyping, which failed to distinguish between the four species. The 16S-23S rDNA intergenic spacer of R. paucula contains the tRNAIle and tRNAAla genes, which are identical to genes described for R. pickettii and R. solanacearum. [1]

Cell structure and metabolism

Interesting features of cell structure; how it gains energy; what important molecules it produces.


Ecology

Habitat; symbiosis; contributions to the environment.

Pathology

R. paucula can cause clusters of nosocomial infections. Treatment of such infections was mainly based on the use of beta-lactams such as cefotaxime, ceftriaxone, piperacillin, and imipenem.[1]

Current Research

References

[1]Moissenet, D., Bidet, P., Garbarg-Chenon, A. Arlet, G., and Vu-Thien1, H. "Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)". J Clin Microbiol. 2001 January; 39(1): 381–384.

Edited by Karolina Wala, Alma Shebib students of M Glogowskiat Loyola University