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Louise Parker Bench D 31/08/2016

Classification

Higher order taxa

Bacteria – Domain – Bacteroidetes – Bacteroidetes – Bacteroidales – Porphyromonadaceae – Porphyromonas

Species

Porphyromonas gingivalis and type strain (consult LPSN http://www.bacterio.net/index.html for this information)

Description and significance

Give a general description of the species (e.g. where/when was it first discovered, where is it commonly found, has it been cultured, functional role, type of bacterium [Gram+/-], morphology, etc.) and explain why it is important to study this microorganism. Examples of citations ^[1], ^[2]

Porphyromonas gingivalis is a Gram-negative anaerobe. It is a major causative agent of the initiation and progression of periodontal disease, a main cause of tooth loss ^[1]. It is often found in deep periodontal pockets.

Genome structure

Select a strain for which genome information (e.g. size, plasmids, distinct genes, etc.) is available.

The Porphyromonas gingivalis strain W83 has a 2,343,479bp genome sequence. It has an average G+C content of 48.3%. 1,990 open reading frames were identified in the genome, making up 85% of the genome. 1,075 of these were assigned biological role categories. - (Complete Genome Sequence of the Oral Pathogenic Bacterium Porphyromonas gingivalis Strain W83)Distinct genes

Cell structure and metabolism

Cell wall, biofilm formation, motility, metabolic functions.

Ecology

Aerobe/anaerobe, habitat (location in the oral cavity, potential other environments) and microbe/host interactions.

Pathology

Do these microorganisms cause disease in the oral cavity or elsewhere?

Application to biotechnology

Bioengineering, biotechnologically relevant enzyme/compound production, drug targets,…

Current research

Summarise some of the most recent discoveries regarding this species.

References

References examples

1. [http://dnaresearch.oxfordjournals.org/content/15/4/215.long#ref-14 Naito, M., Hirakawa, H., Yamashita, A., Ohara, N., Shoji, M., Yuritake, H., Nakayama, K., Toh, H., Yoshimura, F., Kuhara, S., Hattori, M., Hayashi, T., Nakayama, K. (2008) Determination of the Genome Sequence of Porphyromonas gingivalis Strain ATCC 33277 and Genomic Comparison with Strain W83 Revealed Extensive Genome Rearrangements in P. gingivalis. DNA Res 4:215-225

bold number - issue/volume?

1. Sahm, K., MacGregor, B.J., Jørgensen, B.B., and Stahl, D.A. (1999) Sulphate reduction and vertical distribution of sulphate-reducing bacteria quantified by rRNA slotblot hybridization in a coastal marine sediment. Environ Microbiol 1: 65-74.

2. Human Oral Microbiome

This page is written by Louise Parker for the MICR3004 course, Semester 2, 2016