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Louise Parker Bench D 31/08/2016

Classification

Higher order taxa

Bacteria – Domain – Bacteroidetes – Bacteroidetes – Bacteroidales – Porphyromonadaceae – Porphyromonas

Species

Porphyromonas gingivalis and type strain (consult LPSN http://www.bacterio.net/index.html for this information)

Description and significance

Give a general description of the species (e.g. where/when was it first discovered, where is it commonly found, has it been cultured, functional role, type of bacterium [Gram+/-], morphology, etc.) and explain why it is important to study this microorganism. Examples of citations ^[1], ^[2]

Porphyromonas gingivalis is a Gram-negative anaerobe. It is a major causative agent of the initiation and progression of periodontal disease, a main cause of tooth loss ^[1]. It is often found in deep periodontal pockets.

Genome structure

Select a strain for which genome information (e.g. size, plasmids, distinct genes, etc.) is available.

The Porphyromonas gingivalis strain W83 has a 2,343,479bp genome sequence. It has an average G+C content of 48.3%. 1,990 open reading frames were identified in the genome, making up 85% of the genome. 1,075 of these were assigned biological role categories. - (Complete Genome Sequence of the Oral Pathogenic Bacterium Porphyromonas gingivalis Strain W83)Distinct genes

Cell structure and metabolism

Cell wall, biofilm formation, motility, metabolic functions.

Ecology

Aerobe/anaerobe, habitat (location in the oral cavity, potential other environments) and microbe/host interactions.

Pathology

Do these microorganisms cause disease in the oral cavity or elsewhere?

Application to biotechnology

Bioengineering, biotechnologically relevant enzyme/compound production, drug targets,…

Current research

Summarise some of the most recent discoveries regarding this species.

References

References examples

1. Naito, M., Hirakawa, H., Yamashita, A., Ohara, N., Shoji, M., Yuritake, H., Nakayama, K., Toh, H., Yoshimura, F., Kuhara, S., Hattori, M., Hayashi, T., Nakayama, K. (2008) Determination of the Genome Sequence of Porphyromonas gingivalis Strain ATCC 33277 and Genomic Comparison with Strain W83 Revealed Extensive Genome Rearrangements in P. gingivalis. DNA Res 4:215-225.

bold number - issue/volume?

1. Sahm, K., MacGregor, B.J., Jørgensen, B.B., and Stahl, D.A. (1999) Sulphate reduction and vertical distribution of sulphate-reducing bacteria quantified by rRNA slotblot hybridization in a coastal marine sediment. Environ Microbiol 1: 65-74.

2. Human Oral Microbiome

This page is written by Louise Parker for the MICR3004 course, Semester 2, 2016