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Esther Chua | Bench E | 31 Aug 2016

Classification

Higher order taxa

Bacteria - Proteobacteria - Betaproteobacteria - Neisseriales - Neisseriaceae - Kingella - Kingella oralis

Species

Kingella oralis

Type strain: strain UB-38 = ATCC 51147= CCUG 30450 = CIP 103803

Description and significance

Kingella oralis was originally described by Chen et al.(1990) as Eikenella corrodens -like isolates. In the original 1993 classification paper, this species was named K. orale, but the spelling was changed to "oralis" in 1994 (Dewhirst et at., 1993,1994). K. oralis are gram-negative facultative (aerobic and anaerobic growth) organism thats grows in mesophillic environments. They are found in the human oral cavity in dental plaque, and is associated with periodontitis. They also produce corroding colonies.

Genome structure

Kingella Oralis strain ATCC 51147 does not encode for chromosomes and plasmids. It has a total length of about 2.41mb, encodes for 2,315 proteins and has a GC content of 54.3%. Sequencing results show that ATCC51147 consists of 5 scaffolds (Scfld 0-4) with no gaps between, an assembly gap length of 700, and 12 contigs. It has 3,165 coding genes and 52 non-coding genes. However the functions of these genes are not yet known.

This strain has contains specialty genes that are of particular interest, such as antibiotic resistance genes, drug targets, virulence factors, and human homologs.

Select a strain for which genome information (e.g. size, plasmids, distinct genes, etc.) is available.

Cell structure and metabolism

Cell wall, biofilm formation, motility, metabolic functions.

Kingella oralis are gram negative rods or cocobacilli that can form pairs or chains. It has a cell wall that consists of an outer membrane containing lipopolysaccharides, a periplasmic space with a peptidoglycan layer, and an inner, cytoplasmic membrane. Cells have monopolar fimbriae and form spreading colonies. Cells can be aerobic or facultatively anaerobic.

They are oxidase positive and catalase negative

Ecology

Aerobe/anaerobe, habitat (location in the oral cavity, potential other environments) and microbe/host interactions.

Pathology

Do these microorganisms cause disease in the oral cavity or elsewhere?

Application to biotechnology

Bioengineering, biotechnologically relevant enzyme/compound production, drug targets,…

Current research

Summarise some of the most recent discoveries regarding this species.

References

1. (2016) Kingella oralis (ID 1949)-Genome-NCBI 2. Yagupsky, P. Kingella species. Antimicrobe

References examples

1. Sahm, K., MacGregor, B.J., Jørgensen, B.B., and Stahl, D.A. (1999) Sulphate reduction and vertical distribution of sulphate-reducing bacteria quantified by rRNA slotblot hybridization in a coastal marine sediment. Environ Microbiol 1: 65-74.

2. Human Oral Microbiome

This page is written by <Esther Chua> for the MICR3004 course, Semester 2, 2016