User:Ywcheng

A Microbial Biorealm page on the genus Mycoplasma arthritidis Contents [hide]

   * 1 Classification
         o 1.1 Higher order taxa
         o 1.2 Species
   * 2 Description and significance
   * 3 Genome structure
   * 4 Cell structure and metabolism
   * 5 Ecology
   * 6 Pathology
   * 7 Application to Biotechnology
   * 8 Current Research
   * 9 References

Classification Higher order taxa

Cellular organisms; Bacteria; Firmicutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma arthritidis

NCBI: Taxonomy Mycoplasma arthritidis

Strains

Rats:158L3-1 Mice:158L3-1

Description and significance

Among all prokaryotes, Mycoplasma is the smallest genus that is capable of self-replication. Comparing to other bacteria, Mycoplasma has the smallest genome size, which is about 1/5 size of E. coli. Currently, there are more than 70 species with size range from 0.125-0.82 microns and more than 150 species in total. Mycoplasma has many different structual forms: filamentous, coccoidal, spherical and granular. In fact, the word, "Mycoplasma" comes from its filamentous and branching structures, which literally means "fungus form." Mycoplasma is separated into a different taxonomic group mainly because of its two unique characteristics, which are small genome size and lack of cell wall. This lacking of cell wall provides plasticity, which means that its adaptation to the environment is more flexible. Due to the lack of cell wall, Mycoplasma evolves to form a more well-adavanced cell membrane for its immune response and parasitic nature. Early studies of Mycoplasma were discovered while isolating a species that was a bovine pathogen in 1898. (Mycoplasma...a Bacterial Enigma) The discovery of Mycoplasma eventually led to the discovery of one of its species Mycoplasma arthritidis. The research on M. arthritidis is still new, and its genome sequence is still in progress. However, its genome size is found to be 840kb, GC content is 31% of DNA, and its habitat is rodents. (Mycoplasma Molecular Biology pg. 29) Several studies have shown that the ideal pH for M. arthritidis strain 158L3-1 is 7.6-8.0 and ideal tempearture is 37 degrees in Celsius. Further investigations into the metabolic, genetic and physiological property have been progressively explored in animals, plants, and humans. One of the most popular studies on M. arthritidis is the unique features of its surface proteins. (

Genome structure M. arthritidis is found to be quite small in the taxonomic group of Mollicutes. Its genome size is found to be 840kb and the percentage of GC in DNA is 31%. The genome sequencing of M. arthritidis strain 158L-3 is still in progress. Many cellular features have already been found. M. arthritidis 158L3-1 is a spherical Gram - bacteria. Its arrangement is in singles and it has no endospores or motility. Due to its parasitic nature, there are many unique adhesins and surface proteins, such as MAV1 and MAV2 that are responsible for its capability of attaching to its host cells.

Cellular structure and metabolism M. arthritidis grows in a neutral pH environment (7.0) and in an optimal temperature of 37 degrees Celsius. It is a facultative aerobe, which means that it is capable of growing in either in the presence or the absence of oxygen. However, it tends to favor the absence of oxygen more. (mycoplasma...a bacterial enigma) For M. arthritidis, it has been found that it undergoes a tricarboxylic acid cycle. It also has a high arginine deminase activity of 0.65 micromoles per minue per milligram of proteins. This indicates that it undergoes metabolism in a strictly anaerobic condition. (activities oxidative in mycoplasma) M. arthritidis highly depends on the media it is growing on. For instance, in fibrous tissue, it tends to create a dense accumulation in the center, which lightens up as it spreads out. It utilizes sugars (glucose, fructose, mannose, maltose, starch, and glycogen), proteins, amino acids of both purines and pyrimidines, vitamins, nucleic acids, pentose and other inorganic materials for growth. If there is a choice for energy, carbohydrate would be its favorite food. (mycoplasma...a bacterial enigma) It is quite difficult to understand its mechanism of metabolism, but studies have shown that M. arthritidis has very low activities of pyruvate dehydrogenase complex and pyruvate dehydrogenase. This indicates that M. arthritidis does not undergo the citric acid cycle or glycolytic pathway for energy and synthesis of ATP. (They posses a deficient energy-yielding pathway) (mycoplasmal molecular biology pg. 31)

Ecology

Describe any interactions with other organisms (included eukaryotes), contributions to the environment, effect on environment, etc.

Pathology How does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.

Application to Biotechnology Does this organism produce any useful compounds or enzymes? What are they and how are they used? Current Research

Enter summaries of the most recent research here--at least three required References

[Sample reference] Takai, K., Sugai, A., Itoh, T., and Horikoshi, K. "Palaeococcus ferrophilus gen. nov., sp. nov., a barophilic, hyperthermophilic archaeon from a deep-sea hydrothermal vent chimney". International Journal of Systematic and Evolutionary Microbiology. 2000. Volume 50. p. 489-500.

Edited by student of Rachel Larsen