Vickie Nguyen and Haylie Beall
Classification
Bacteria, Bacillus[Others may be used. Use NCBI link to find]
Bacillus subtilis
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NCBI: Taxonomy |
According to BLAST using a genome sequence from PCR, our organism has been classified as Bacillus subtilis
Habitat Information
The organism was isolated from a 10^-3 dilution of soil harvested from a patch of soil in Buda, TX. The soil sample was collected on a sunny afternoon in February 2016. The weather showed low humidity and a temperature of 83 degrees Fahrenheit. The soil dilution was plated on a TSA plate and cultured for one week. Bacterial colonies were transferred to a master patch plate and tested for antibiotic resistance. The organism was chosen based on its antibiotic resistance to E. coli.
Description and Significance
Describe the appearance (colonial and cellular), possible antimicrobial activity etc. of the organism, and why the organism might be significant.
Genome Structure
Describe the size and content of the genome. How many chromosomes? Circular or linear? Other interesting features? What is known about its sequence? Include S Ribosomal sequence that you obtained from PCR and sequencing here.
Cell Structure, Metabolism and Life Cycle
Interesting features of cell structure; how it gains energy; what important molecules it produces.
Physiology and Pathogenesis
Biochemical characteristics, enzymes made, other characteristics that may be used to identify the organism; contributions to environment (if any).
If relevant, how does this organism cause disease? Human, animal, plant hosts? Virulence factors, as well as patient symptoms.
References
Author
Page authored by Vickie Nguyen and Haylie Beall, students of Prof. Kristine Hollingsworth at Austin Community College.
