Candidatus Cardinium

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Fig. 1. Electron micrographs of ‘Candidatus Cardinium’ in ovaries of Encarsia parasitoid wasps. (a) Bacteria in a follicle cell of E. hispida. (b–d) Bacteria in sexual E. pergandiella nurse cells. (e) Bacteria in an E. hispida oocyte. Arrowheads point to MLSs; l, lipid-filled vacuoles in the oocyte; m, mitochondrion; p, protein. Bars, 0·25 µm.[1]

Classification

Domain (Bacteria); Super Phylum (Bacteroidetes/Chlorobi group); Phylum (Cytophaga-Flavobacterium-Bacteroides); Class (Bacteroidetes); Order (Bacteroidales); Family (Bacteroidaceae); Genus (Candidatus Cardinium)

Species

Candidatus Cardinium

Description and Significance

‘Candidatus Cardinium’ is a bacterium from the Bacteriodetes group. It is involved in reproduction alterations of arthropod host organisms including cytoplasmic incompatibility, parthenogenesis, and feminization.[3]

This bacterium is often located within the reproductive tissues of the host. It is often found with other arthropod-associated Bacteroidetes that alter host biology (ex. Wolbachia). Distantly related hosts can harbor closely related Cardinium. Closely related Cardinium also tend to cluster with closely related hosts. [4]

Current research has shown 'Cardinium' to be an endosymbiot capable of altering host sexuality, resultant offspring as well as possible antibiotic resistance. Research on this microbe is still in its infancy but further research could help explain the natural endosymbiotic flora of arthropods. [5]

Genome Structure

‘Candidatus Cardinium’ has linear 1364 bp DNA sequence. There are 3 main varieties of Cardinium which differ in their 16s rRNA sequences.

FIG. 4. Relationship of Cardinium groups A, B, and C. Each rectangular box represents a Cardinium group. Identities of 16S rRNA gene nucleotide sequences (1,447 or 1,448 bp) and in GyrB amino acid sequences (286 aa) are indicated on the lines which connect the groups. The nucleotide or amino acid identities were calculated with Genetyx version 9.1 software (Genetyx Corporation, Tokyo).[2]

Cell Structure, Metabolism and Life Cycle

'Cardinium' exists as both a rod and circular shaped bacterial endosymbiot 0.3 - 2.1µm in length with microfilament-like structures extending from the body. It is transmitted vertically from mother to offspring and enhances its own transmission by altering the host biology causing feminization that is possibly functional for males as well as parthenogenesis. Like other endosymbiots, it may add to the fitness of the host organism by supplying nutrients, adding heat resistance or otherwise reduce possibly predation. It has been hypothesized that it confers a slight insectiside resistance to the host. [6]

When 'Cardinium' infects a male host, it has the capacity to kill, functionally, or genetically convert the organism to a female during microbe development. In the case of a genetic conversion, infected males who mate are able to produce very few offspring due to cytoplasmic incompatibility. [7]

Ecology and Pathogenesis

This bacteria is located within the reproductive tissues of the host organism. Commonly found in arthropods, mites and spiders. Nearly 7% of all arthropods and 30% of all mites and spiders contained this microbe as a member of its endosymbiotic flora.

Often transmitted from mother to daughter.‘Candidatus Cardinium' has the capacity to feminize the host organism. Feminization can be functional or genetic alone. In some cases, it has been shown to increase fecundity of the female host. This endosymbiont does not appear to have a negative effect on the host. It has been hypothesized that it confers a slight insectiside resistance to the host. [8]

References

[1] Zchori-Fein, E, S. Perlman, S. Kelly, N. Katzir, M, Hunter. "Characterization of a ‘Bacteroidetes’ symbiont in Encarsia wasps (Hymenoptera: Aphelinidae): proposal of ‘Candidatus Cardinium hertigii’ ". International Journal of Systematic and Evolutionary Microbiology. 2004. Volume 54. p. 961-968.

[2] Kenyon, S, S. Hunter, M. "Manipulation of oviposition choice of the parasitoid wasp, Encarsia pergandiella, by the endosymbiotic bacterium Cardinium ". European Society for Evolutionary Biology. 2006. Volume 20. p. 707-716.

[3] Zchori-Fein, E, S. Perlman. "Distribution of the bacterial symbiont Cardinium in arthropods.". Molecular Ecology. 2004. Volume 13. p. 2009-16.

[4] Chigira, A, K. Miura. "Detection of 'candidatus Cardinium' bacteria from the haploid host Brevipalpus californicus (Acari: Tenuipalpidae) and effect on the host.". Experimental & Applied Acarology. 2005. Volume 37. p. 107-16.

[5] Chiel, E, Y. Gottlieb, E. Zchori-Fein,N.Mozes-Daube, N. Katzir, M. Inbar, M. Ghanim. "Biotype-dependent secondary symbiont communities in sympatric populations of Bemisia tabaci." Bulletin of Entomological Research. 2007. Volume 97. p. 407-13.

[5] Matalon, Y, N. Katzir, Y. Gottlieb, V. Portnoy, E. Zchori-Fein. "Cardinium in Plagiomerus diaspidis (Hymenoptera: Encyrtidae)." Journal of Invertebrate Pathology. 2007. Volume 96. p. 106-8.

[6] Nakamura, Y, S. Kawai, S. Ito, T. Gotoh, R. Kisimoto, T. Yanase, Y. Matsumoto, D. Kageyama, H.Noda. "Prevalence of Cardinium bacteria in planthoppers and spider mites and taxonomic revision of "Candidatus Cardinium hertigii" based on detection of a new Cardinium group from biting midges.." Applied Environmental Microbiology. 2009. Volume 21. p. 6757-63.

Author

Page authored by Benjamin Lowe & Brian Lovett, students of Prof. Jay Lennon at Michigan State University.

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