Halorhabdus utahensis

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Classification

Archaea; Euryarchaeota; Halobacteria; Halobacteriales; Halobacteriaceae; Halorhabdus


Species

Halorhabdus utahensis

Description and Significance

Halorhabdus (salt-loving rod) utahensis (pertaining to the state of Utah) was isolated from a sediment sample that was collected in the southern arm of the hypersaline Great Salt Lake, Utah, USA. It is a motile, gram-negative, extreamly halophilic archaeon that forms red, circular colonies. It grows in temperatures between 17 and 55°C, with optimal growth occurring at 50°C. It can also grow over a pH range of 5.5-8.5 with the optimal pH value between 6.7 and 7.1. Halorhabdus utahensis is of interest due to its ability to grow over a wide range of salinity. It can grow anywhere from 6-30% NaCl with an optimum of an astounding 27% (2).

The archaeon utilizes only a limited range of substrates such as glucose, xylose, and fructose, for growth, and is unique in its inability to utilize yeast extract or peptone. Other substances that do not stimulate the organism's growth include organic acids, amino acids, alcohols, glycogen, and starch (2).

Halorhabdus utahensis under Scanning electron micrograph (1)

A research paper in 2011 described an enzyme in H. utahensis that was important in biofuel production. Non-food plant sources of lignocellulosic biomass are being used more frequently in biofuel production. The only problem is being able to efficiently and cost-effectivey degrade this biomass into more simple sugars. Ionic liquids, which is a new class of solvents, have been investigated as being much more efficient to treat the biomass there by yielding a greater amount of sugars. The only issue is that the ionic liquids can interfere with the ability of previous cellulases (from fungi) to perform. The recent discovery of a salt-tolerant cellulase enzyme in H. utahensis has had an effect on reducing the costs and water requirements needed during the IL pretreatment process. The enzyme was discovered using a genome based approach. The enzyme, named Hu-CBH1, surface that contains negatively charged amino acids that help stabilize the enzyme. The enzyme can withstand salt concentrations of up to 5 M NaCl, a pH of 11.5 and is also heat tolerant. The most important characteristic of this enzyme is that it can handle IL concentrations of 20% w/w, including 1-allyl-3-methylimidazolium chloride ([Amim]Cl) (3).

Genome Structure

The entire genome of Halorhabdus utahensis has been sequenced by the DOE Joint Genome Institute (JGI) and is accessible in Genbank. It was sequenced using a combination of Sanger sequencing and also 454 where the error rate of the completed genome sequence is less than 1/100,000. It has been determined that Halorhabdus utahensis contains one main circular chromosome that is 3,116,795 bp long and is comprised of 62.90% G+C content. There are approximately 3,075 genes of which 3,027 are protein encoding genes, 48 are RNA genes, 29 are pseudogenes, and one is an rRNA operon. Some of the genes help to encode Halorhabdus utahensis ability to become pleomorphic (multiple structural forms). It does not need any amino acids to grow and therefore is prototrophic for their acquisition (1). This characteristic utilizes several genes along with the cells resistance to ampicillin, carbenicillin, chloramphenicol, erythromycin, gentamicin, kanamycin, nalidixic acid, neomycin, penicillin, polymyxins, rifampicin, streptomycin, and tetracycline (2).

Cell Structure, Metabolism and Life Cycle

The cells of Halorhabdus utahensis are extremely pleomorphic (can take on multiple shapes), exhibiting anything from irregular coccoid or ellipsoid to triangular, club-shaped or rod-shaped forms. The rod-shaped and ellipsoid cells are 2-10x0.5-1 µm and 1-2x1 µm in size, respectively, and the spherical cells have a diameter of approximately 1 µm. It is usually in a rod shaped form, particularly in its younger years and later in life it becomes increasingly pleomorphic (2). The organism is mobile and has a flagellum, ranging in size from 3 to 5 micro meters. The cells can be distinguished by a negative gram stain. Furthermore, Halorhabdus utahensis has a red color and the pigments are not precisely known but presumed to be, “carotenoids, probably bacterioruberins”. The outer cell layers are proteinaceous (1).

Growth of the cell does not require amino acids, but does require nitrogen and a carbon source (2). The metabolism of Halorhabdus utahensis is aerobic, but is also a facilitative anaerobe. It receives its energy from carbohydrates where the carbon source is from fructose, xylose, and glucose. Not important to the growth of the cell, however a side product of it metabolism is the reduction of nitrate to nitrite. It has been found that it may grow anaerobically by fermentation of glucose (1).

Ecology and Pathogenesis

Growth of H.utahensis with varying NaCl concentration. (2)

Halorhabdus utahensis was isolated from sediment found in the Great Salt Lake in Utah, USA. This microbe is extremely halophilic and is found in an environment with high levels of NaCl. When grown in an anaerobic environment with glucose nitrate was reduced to nitrite, although this did not stimulate the microbe’s growth (2). There were no signs of Halorhabdus utahensis engaging in any kinds of symbiosis with other forms of life.


Halorhabdus utahansis has no pathogenicity towards other organisms (1). However it was found that Halorhabdus utahensis was susceptible to bacitracin and novobiocin but resistance to ampicillin, carbenicillen, chloramphenicol, erythromycin, gentamicin, kanamycin, calidixic acid, neomycin, penicillin, polymyxins, rifampicin, streptomycin, and tetracycline (2).

References

1. Anderson, I., Tindall, B., Pomrenke, H., & Goker, M. (2009). Complete genome sequence of Halorhabdus utahensis type strain (AX-2T). Standards in Genomic Sciences, (1), 218-225.

2. Waino, M., Tindall, B., & Ingvorsen, K. (2000). Halorhabdus utahensis gen. nov., sp. nov., an aerobic, extremely halophilic member of the archaea from Great Salt Lake, Utah. International Journal of Systematic Evolutionary Microbiology, (50), 183-190.

3. Zhang, Tao, Supratim Datta, Jerry Eichler, and Natalia Ivanova. “Identification of a Haloalkaliphilic and Thermostable Cellulase with Improved Ionic Liquid Tolerance.” Green Chemistry 13 (2011): 2083-2090.

Author

Page authored by Elliot Swift, Mark Sherrill, Matt Stoloff, Hannah Arnett, student of Prof. Jay Lennon at Michigan State University.

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