Methanobacterium aarhusense
A Microbial Biorealm page on the genus Methanobacterium aarhusense
Classification
Higher order taxa
Domain: Archaea Kingdom: Archaea Phylum: Euryarchaeota Class: Methanobacteria Order: Methanobacteriales Family: Methanobacteriaceae Genus: Methanobacterium Species: "Methanobacterium aarhusense"
Species
Methanobacterium aarhusense
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NCBI: Taxonomy |
Description and significance
M. aarhusense is mesophilic, moderately halophilic, non-motile, meaning that it is a very picky microbe and prefers temperatures above 30℃. The optimum growth temperature is 45℃. It grows well at pH of 7-5 and 8, however the optimum pH is unknown. Grows well in NaCl concentration range of 100 and 900mM.
Genome structure
DNA G+C content is 34-39 mol %. The strain forms a distinct line of descent within this genus, with Methanobacterium oryzae (95·9 % sequence identity) and Methanobacterium bryantii (95.7% sequence identity).
Cell structure and metabolism
It is 5-18 μm long and 0-7 μm wide, filamentous. It is a gram positive meaning that it has a thick peptidoglycan layer. M. aarhusense does not possess any pili or flagella, keep it non-motile. A large sample has been sequenced and contains 1333 base pairs.
M. aarhusense is a hydrogenotrophic methanogen, meaning that it converts hydrogen to energy as a way of metabolism and produces methane. No growth or methane production was observed at 1 M NaCl. At 50 mM NaCl, growth and methane production were reduced.
Ecology
M. aarhusense is a chemoautotroph, meaning that it produces complex organic compounds from simple substances. Optimal growth temperature is 45℃. Grows at pH 7-5 and 8. Can live in a CaCl concentration of 100 and 900 mM. Isolated from marine sediment in Aarhus Bay, Denmark. An artificial marine medium was created and it consists of three different solutions. Solution A consists of NaCl, 20; MgCl2.6H2O, 3; KCl, 0·7; NH4Cl, 0·2; KH2PO4, 0·2; CaCl2.2H2O, 0·2. Solution B consists of NaHCO3, 2·5 g in 30 ml demineralized water. Lastly, solution C consists of: 1·5 ml l−1 of 0·04 g Na2S.9H2O ml−1 demineralized water. On the agar, the colonies were circular and grayish, with white surrounding it.
References
Boone, D. R. (2001). Genus I. Methanobacterium. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 215–218. Edited by D. R. Boone & R. W. Castenholz. New York: Springer-Verlag.
Goldman AD, Leigh JA, Samudrala R. Comprehensive computational analysis of Hmd enzymes and paralogs in methanogenic Archaea. <http://openi.nlm.nih.gov/detailedresult.php? img=2739858_1471-2148-9-199-1&req=4>
IJSEM May 2004 vol. 54 no. 3 759-763. Methanobacterium aarhusense sp. nov., a novel methanogen isolated from a marine sediment (Aarhus Bay, Denmark). <http://ijs.sgmjournals.org/content/54/3/759/F2.expansion>
Shlimon, Adris Georgis, Michael W. Friedrich, Helge Niemann, Niels Birger Ramsing, Kai Finster. "Methanobacterium aarhusense sp. nov., a novel methanogen isolated from a marine sediment (Aarhus Bay, Denmark)." International Journal Of Systematic and Evolutionary Microbiology 30 January 2004. 9 February 2013 <http://ijs.sgmjournals.org/content/54/3/759.abstract>.
Edited by student of Lisa Moore
