NS Mystery Organism
Classification
Domain; Phylum; Class; Order; family [Others may be used. Use NCBI link to find]
Unknown
Species
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NCBI: Taxonomy |
NS Mystery Organism
Habitat Information
Describe the location and conditions under which the organism was isolated.
Date: 09/06/2017
Time: 18:49:00 CST
Air Temperature: 83 ℉
Humidity: 33%
24 hr rainfall: 0 inches
Pressure: 30.6 inch/ 1,017.0 mb
Solar Radiation: 22.49 ms/m²
Latitude: 30.144673 | Longitude: -97.841463
The organism was isolated at a latitude of 30.144673 and a longitude of -97.841463 on September 6, 2017 at 18:49:00 CST. The conditions under which the NS Mystery Organism was isolated includes it being 83 degrees fahrenheit and 33% humidity. There were 0 inches of rainfall with a solar radiation of 22.49 ms/m^2.
Description and Significance
The colony morphology of NS Mystery Organism has a smooth surface with slightly raised elevation and an entire margin. The organism exhibits creamy yellow color.
Cellular arrangement through microscopic observation is Diplobacilli.
Significance
The antimicrobial activity test has shown a zone of inhibitions with all the antibiotics used which are listed below. Antimicrobial agents used: 1) Sulbactam, 2. Ticracillin 3. Cefoxitin 4. Oxacillin 5. Vancomycin
The Organism was also sensitized by some disinfectants used including Tea tree, PepperMint, Lysol 10% and Bleach 100%.
Genome Structure
PCR and Gel electrophoresis tests were inconclusive, therefore, the results are unknown due to complications from sequence reactions.
Cell Structure, Metabolism and Life Cycle
Gram Stain NS Mystery Organism shows to be Gram-positive under Gram stain reaction test with prominent purple color from Crystal Violet.
Capsule Stain Under Capsule Stain test, the result is negative without any appearance of Capsule.
Endospore test The Organism also shows Negative Endospore test result under Endospore Stain test.
Motility test However, under the Motility test, there was a movement away from the stab line into the medium which indicates a Positive Motility test result. Non-motile organisms which lack flagella, are usually going to form a single line of growth that does not spread into the surrounding area. While a motile bacterium will grow and make a hazy zone around the stab line.
Oxidase test The NS Mystery Organism has shown a Positive test result which indicates Aerobic respiration. It can use oxygen as a terminal electron acceptor in respiration which was proven through a positive oxidase test.
Triple Sugar Iron Agar NS Mystery Organism turns yellow/yellow under Triple Sugar Iron Agar (TSI) test points out fermentation of Lactose and Glucose.
DNA Hydrolysis The Organism produces DNase enzyme under DNA hydrolysis test with a clearing around growth in the agar plate.
Lipid Hydrolysis It also shows to have Lipase enzyme with a weak positive test result of Lipid Hydrolysis.
Physiology and Pathogenesis
Mannitol Salt Agar The Mannitol Salt Agar result for the organism indicated a positive result. The medium shows good yellow growth and is presumed Staphylococci because it is not inhibited by NaCl. The organism produces acid from Mannitol fermentation and is presumed possible pathogenic Staphylococcus aureus.
Lipid Hydrolysis Lipid hydrolysis was used to detect lipolytic bacteria, especially in high-fat dairy products. Lipase allows the organisms that produce it to break down lipids into smaller fragments. The test showed a weak positive.
Starch Hydrolysis Results : Inconclusive/Unknown
Phenol Red Broth: Results : Negative Fermentation.
Casein Hydrolysis: Results : Negative. There was no clearing around the organism which indicates a negative test result.
Gelatin Hydrolysis: Results : Negative. The organism shows inability to produces Gelatinase.
Eosin methylene blue agar EMB test is used to encourage the growth of fecal coliforms, to test drinking water. The result is based on lactose fermentation. The results were positive since pink colonies formed, indicating slow and weak fermentation.
Phenylethyl Alcohol Agar The organism is not inhibited by phenylethyl alcohol as proven with Phenylethyl Alcohol Agar.
References
Author
Page authored by Suchanart Apisaksiri and Sally Phonthaasa, students of Prof. Kristine Hollingsworth at Austin Community College.
