Pseudomonas granadensis
Classification
Domain: Bacteria
Phylum: Proteobacteria
Class: Gammaproteobacteria
Order: Pseudomonadales
Family: Pseudomonadaceae
Genus: Pseudomonas
Other Names:
- DSM 28040
- LMG 27940
- Pseudomonas granadensis Pascual et al. 2015
- Pseudomonas sp. F-278,770
- strain F-278,770
Species
|
NCBI: Taxonomy |
Genus species: Pseudomonas granadensis
Habitat Information
Latitude: 30.26 degrees Longitude: 97.69 degrees
It was a clear day with a temperature of 64 degrees in the area of Govalle, East Austin on January 25th 2018. A ziplock bag was used to collect the soil from the area mostly from the surface about one inch deep. The location of the soil sample chosen was in a neighborhood field often frequented by dogs. Rainfall was 0.0" and the pressure was 35.35". The description of the location was mostly Bergstrom soils and urban land. 0-2% slopes and rarely flooded.
Description and Significance
The colonial characteristics of the organism are described as follows: circular, convex, and entire margins. Caramel in color with no extracelluar pigment and a shiny, mucoid appearance. To determine the extent of the antimicrobial activity of the organism, further testing would be needed as there was only some clearing when tested.
A unique characteristic of this gram-negative is its polar flagellum, although some have two polar flagella. There was no discernible arrangement of the cocci bacterium
Genome Structure
Pseudomonas granadensis strain 26C3 16S ribosomal RNA gene, partial sequence
(Reverse)
ACCGTCCTCCCGAAGGTTAGACTAGCTACTTCTGGTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGA
ACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCGACTTCACGCAGTCGAGTTGCAGACTGCGATCCGGACTACG
ATCGGTTTTATGGGATTAGCTCCACCTCGCGGCTTGGCAACCCTTTGTACCGACCATTGTAGCACGTGTGTAGCCCAGGCCGTA
AGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCTCCTTAGAGTGCCCACCATTACGTGCTG
GTAACTAAGGACAAGGGTTGCGCTCGTTACGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTG TCTCAATGTTCCCGAAGGCACCAATCCATCTCTGGAAAGTTCATTGGATGTCAAGGCCTGGTAAGGTTCTTCGCGTTGCTTCGAA
TTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCA
ACTTAATGCGTTAGCTGCGCCACTAAGAGCTCAAGGCTCCCAACGGCTAGTTGACATCGTTTACGGCGTGGACTACCAGGGTAT
CTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGTGGTCGCCTTCGCCACTGGTGTTCCTTCCTA
TATCTACGCATTTCACCGCTACACAGGAAATTCCACCACCCTCTACCATACTCTAGCTCGCCAGTTTTGGATGCAGTTCCCAGGT
TGAGCCCGGGGATTTCACATCCAACTTAACGAACCACCTACGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTC
TGTATTACCGCG
Cell Structure, Metabolism and Life Cycle
Studies have shown that glucose was the optimum carbon source and ammonium oxalate the optimum nitrogen source of this bacterium. It is an oxidase- and catalase-positive, aerobic, with a respiratory type of metabolism with oxygen as the terminal electron acceptor. It was shown to produce (+)-γ-lactamase to hydrolyze racemic (+/−)-γ-lactam from several soil samples using (+/−)-γ-lactam as the only carbon source.
Physiology and Pathogenesis
BIOCHEMICAL TEST RESULTS
- Phenol Red Broth Tests: Glucose: positive; Lactose: negative; Sucrose: negative
- Starch Hydrolysis Test: negative
- Casein Hydrolysis Test: negative
- Gelatin Hydrolysis Test: positive
- DNA Hydrolysis Test: slight positive
- Lipid Hydrolysis Test: positive
- Methyl Red Test: negative
- Voges Proskauer Test: negative
- Citrate Test: positive
- SIM Tests: negative for all
- Nitrate Reduction: negative
- Urea Hydrolysis: negative
- Triple Sugar Iron Agar: negative for all
- Oxidase Test: negative
- Eosin Methylene Blue Agar (EMB) Test: positive
- Hektoen Enteric Agar (HE) Test: negative
- MacConkey Agar Test: negative
- Decarboxylation Tests: Arginine: no change; Lysine: negative; Orinithine: negative
- Phenylalanine Deaminase Test: negative
- Catalase Test: negative
- Blood Agar Test: slight positive
- Mannitol Salt Agar (MSA) Test: negative
- Phenylethyl Alcohol Agar (PEA) Test: negative
- Bacitracin/Optochin Susceptibility Test: Bacitracin: resistant; Optochin: resistant
- Bile Esculin Test: negative
- 6.5% Salt Tolerance Test: slight positive
References
https://www.sciencedirect.com/science/article/pii/S1381117716302880 Wang, J. and Jin L. "Isolation of Pseudomonas granadensis B6 with (+)-γ-lactamase and whole cell resolution of racemic γ-lactam"."Journal of Molecular Catalysis B: Enzymatic". 2016. Volume 133. p. S114-S118.
http://ijs.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.069260-0 Pascual, J., Garcia-Lopez, M., Bills, G., and Genilloud, O. "Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain". International Journal of Systematic and Evolutionary Microbiology. 2015. Volume 65. p. 625-632.
https://www.ncbi.nlm.nih.gov/pubmed/25410940, "Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain". PubMed.gov. Epub 2014 Nov 19.
Author
Page authored by Diane Marques and Emma Riegle, students of Prof. Kristine Hollingsworth at Austin Community College.
