Domain: Eukaryota Kingdom: Fungi Subkingdom: Dikarya Phylum: Ascomycota Subphylum: Taphrinomycotina Class: Schizosaccharomycetes Order: Schizosaccharomycetales Family: Schizosaccharomycetaceae Genus: Schizosaccharomyces Species: cryophilus
Background & Description
Fission yeast are unicellular eukaryotes that undergo division by medial fission rather than budding. Normally, propagation is mitotic as haploids, but under nitrogen starvation, sexual union between two haploid cells of opposite mating-types occurs, thus deriving the name fission yeast. The first physical step in the process is cellular elongation and the formation of conjugation tubes towards pheromones secreted by opposite mating-type cells.2 Conjugation of two opposite mating-types creates a diploid zygote which will undergo meiosis to produce four haploid nuclei which become encapsulated by spore walls.3
Schizosaccharomyces cryophilus is specific fission yeast of the genus Schizosaccharomyces that grows at lower temperatures than other known fission yeasts. Morphologically Schizosaccharomyces cryophilus is very similar to Schizosaccharomyces octosporus, but displays unique differences phenotypically and genotypically.
Genome Structure 4
Schizosaccharomyces cryophilus contains a deviation of the D1/D2 divergent domain of the LSU rRNA gene, the RNA subunit of Ribonuclease P (Rnase P), and the ITS elements from Schizosaccharomyces octosporus. 5,6,7,8,9, Genome sequencing between the two species revealed the following genetic differences in the aforementioned loci:
D1/D2 divergent domain of the LSU rRNA gene: 25 nucleotide substitutions and 3 indels
RNA subunit on RNase P: 15 nucleotide substitutions and 3 indels
ITS elements: ITS1: 95 nucleotide substitutions and 66 indels ITS2: 84 nucleotide substitutions and 51 indels
Restriction enzyme digests can also be used in conjunction with gel electrophoresis to confirm differing band patterns between S. cryophilus and S. octosporus.
Metabolism, Physical Structure, & Function 4
Unlike Saccharomycotina, fission yeasts cannot use ethanol as a primary carbon source, suggesting independent evolution. The gene content between budding yeasts and fission yeasts indicates that the glyoxylate cycle, loss of glycogen biosynthesis, fewer glycolytic paralogs, loss of phosphoenolpyruvate carboxykinase, lack of expanded adh genes, and lack of transcriptional regulators of glucose repression are what prohibit fission yeasts from using ethanol as a primary carbon source unlike Saccharomycotina.10 The loss of the phosphoenolpyruvate carboxykinase and adh genes prevents the use of pyruvate for respiration, producing ethanol not as a consumable by-product, but as a waste product. The expression of ald genes in fission yeast indicates there is an alternative pathway for acetyl-coA production in fission yeast and that fission yeast are highly dependent upon glucose rather than ethanol for acetyl-coA manufacture.11
S. cryophilus growth occurs at the optimal temperature of 25°C, compared to the optimal temperature of 32°C for Schizosaccharomyces japonicas, Schizosaccharomyces pombe, and S. octosporus. Additionally, S. cryophilus cannot proliferate at temperatures higher than 25°C, while S. japonicas, S. pombe, and S. octosporus have a growth range of 18°C to 36°C.
Colonies of S. cryophilus on agar are cream-colored and almost butter-like in appearance, which contrasts to the smooth, rounded colonies of S. pombe and the foam-like colonies of S. octosporus. Other differences between S. cryophilus and S. octosporus include the ability to ferment D-glucose, maltose, and sucrose in S. cryophilus as in S. pombe, but not D-galactose, as well as the ability to hydrolyze urea in S. cryophilus and S. pombe.
Chockalingam, Evvie, and S. Subramanian. “Utility of Eucalyptus Tereticornis (Smith) Bark and Desulfotomaculum Nigrificans for the Remediation of Acid Mine Drainage.” Bioresource Technology 100, no. 2 (January 2009): 615–621. doi:10.1016/j.biortech.2008.07.004.
“Genus Desulfotomaculum - Hierarchy - The Taxonomicon.” Accessed November 5, 2013. http://taxonomicon.taxonomy.nl/TaxonTree.aspx?id=229.
Kaksonen, Anna H., Stefan Spring, Peter Schumann, Reiner M. Kroppenstedt, and Jaakko A. Puhakka. “Desulfotomaculum Thermosubterraneum Sp. Nov., a Thermophilic Sulfate-reducer Isolated from an Underground Mine Located in a Geothermally Active Area.” International Journal of Systematic and Evolutionary Microbiology 56, no. 11 (November 1, 2006): 2603–2608. doi:10.1099/ijs.0.64439-0.
Liu, Yitai, Tim M. Karnauchow, Ken F. Jarrell, David L. Balkwill, Gwendolyn R. Drake, David Ringelberg, Ronald Clarno, and David R. Boone. “Description of Two New Thermophilic Desulfotomaculum Spp., Desulfotomaculum Putei Sp. Nov., from a Deep Terrestrial Subsurface, and Desulfotomaculum Luciae Sp. Nov., from a Hot Spring.” International Journal of Systematic Bacteriology 47, no. 3 (July 1, 1997): 615–621. doi:10.1099/00207713-47-3-615.
Moser, Duane P, Thomas M Gihring, Fred J Brockman, James K Fredrickson, David L Balkwill, Michael E Dollhopf, Barbara Sherwood Lollar, et al. “Desulfotomaculum and Methanobacterium Spp. Dominate a 4- to 5-kilometer-deep Fault.” Applied and Environmental Microbiology 71, no. 12 (December 2005): 8773–8783. doi:10.1128/AEM.71.12.8773-8783.2005.
Ogg, Christopher D, and Bharat K C Patel. “Desulfotomaculum Varum Sp. Nov., a Moderately Thermophilic Sulfate-reducing Bacterium Isolated from a Microbial Mat Colonizing a Great Artesian Basin Bore Well Runoff Channel.” 3 Biotech 1, no. 3 (October 2011): 139–149. doi:10.1007/s13205-011-0017-5.
Pikuta, E, A Lysenko, N Suzina, G Osipov, B Kuznetsov, T Tourova, V Akimenko, and K Laurinavichius. “Desulfotomaculum Alkaliphilum Sp. Nov., a New Alkaliphilic, Moderately Thermophilic, Sulfate-reducing Bacterium.” International Journal of Systematic and Evolutionary Microbiology 50 Pt 1 (January 2000): 25–33.
 Leupold U. Sex appeal in fission yeast. Current Genetics. 1987; 12:543–545.
 Tanaka K, Hirata A. Ascospore development in the fission yeasts Schizosaccharomyces pombe and S. japonicas. J Cell Sci. 1982; 56:263–279. [PubMed: 7166567]
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<ref> tag Cho M, Yoon JH, Kim SB, Park YH. Application of the ribonuclease P (RNase P) RNA gene sequence for phylogenetic analysis of the genus Saccharomonospora. Int J Syst Bacteriol. 1998; 48(Pt 4):1223–1230. [PubMed: 9828424]
 Haas ES, Brown JW. Evolutionary variation in bacterial RNase P RNAs. Nucleic Acids Res. 1998; 26:4093–4099. [PubMed: 9722626]
 Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. 1998; 73:331–371.
 Iwen PC, Hinrichs SH, Rupp ME. Utilization of the internal transcribed spacer regions as molecular targets to detect and identify human fungal pathogens. Med Mycol. 2002; 40:87–109.[PubMed: 11860017]
 Nilsson RH, Kristiansson E, Ryberg M, Hallenberg N, Larsson KH. Intraspecific ITS variability in the Kingdom Fungi as expressed in the international sequence databases and its implications for molecular species identification. Evolutionary Bioinformatics. 2008; 2008:193–201.
 Rhind N et al., Comparative Functional Genomics of the Fission Yeasts. Science. 2011 May 20; 332(6032): 930–936.
 DeRisi JL, Iyer VR, Brown PO. Science. 1997; 278:680. [PubMed: 9381177]
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- 1 Chang F, Nurse P. How fission yeast fission in the middle. Cell. 1996; 84:191–194. [PubMed: 8565064]